Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis

Guan, Peng; Sun, Zhiguo; Luo, Liting; Zhou, Jian; Yang, Song; Zhao, Ya-Shuo; Yu, Fu-Yang; An, Ji-Ren; Wang, Na; Ji, En-Sheng

doi:10.1016/j.lfs.2019.04.005

Cited by 30 publications

(18 citation statements)

References 46 publications

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“…Several studies have investigated the role of H 2 treatment in autophagy. In detail, Guan et al ( 39 ) reported that H 2 treatment induced autophagy and protected Sprague-Dawley rats against chronic intermittent hypoxia-induced renal dysfunction. In addition, inhaling 2% H 2 resulted in an increase in the expression of LC3BII and attenuated sepsis-induced liver injury ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Suppression of autophagy facilitates hydrogen gas‑mediated lung cancer cell apoptosis

et al. 2020

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Our previous study found that hydrogen gas (H 2) could efficiently inhibit lung cancer progression; however, the underlying mechanisms still remains to be elucidated. The present study aimed to explore the roles of H 2 in lung cancer cell autophagy, and reveal the effects of autophagy on H 2-mediated lung cancer cell apoptosis and the underlying mechanisms. The expression levels of proteins associated with cell apoptosis and autophagy were detected using western blot analysis. Cell autophagy was inhibited by 3-methyladenine treatment or Beclin1 downregulation, while rapamycin was used to induce autophagy. Cell growth and apoptosis were detected using the Cell Counting Kit-8 and flow cytometry assays, respectively. The results demonstrated that cell apoptosis and autophagy were significantly enhanced in the A549 and H1975 lung cancer cell lines treated with H 2. However, autophagy enhancement weakened H 2 roles in promoting cell apoptosis and vice versa. In addition, it was found that H 2 treatment induced marked decreases in the protein expression levels of phosphorylated STAT3 and Bcl2, and overexpression of STAT3 abolished H 2 roles in promoting cell apoptosis and autophagy. Overall, the present study revealed that H 2 can promote lung cancer cell apoptosis and autophagy via inhibiting the activation of STAT3/Bcl2 signaling and suppression of autophagy can enhance H 2 roles in promoting lung cancer cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Suppression of autophagy facilitates hydrogen gas‑mediated lung cancer cell apoptosis

et al. 2020

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“…Zhuang et al [ 46 ] showed that H 2 treatment downregulated the expression of phosphomammalian target of rapamycin (p-mTOR)/mTOR and p62 in LPS-treated neuroglial cells and increased the expression of phospho-AMP-activated protein kinase (p-AMPK)/AMPK, light chain 3 (LC3) II/LC3 I, triggering receptor expressed on myeloid cells 2 (TREM-2), and Beclin-1 to activate autophagy and attenuate neuroinflammation in sepsis. Guan et al [ 47 ] revealed that H 2 can ameliorate chronic intermittent hypoxia-induced kidney injury by decreasing ER stress and inducing autophagy by inactivating oxidative stress-dependent p38 and JNK MAPKs. H 2 can also inhibit autophagy by downregulating NF- κ B, Beclin-1, and MAPK and upregulating the HO-1, mTOR, and LC3B signaling pathways.…”

Section: Mechanisms Of the Action Of Hmentioning

confidence: 99%

“…Although many studies have investigated the effectiveness of H 2 on various diseases related to oxidative stress, little is known about the influence of H 2 on exercise-induced oxidative stress. In 2012, Aoki et al found that HW reduced blood lactate levels and improved exercise-induced decline of muscle function in ten male soccer players [ 47 ]. Yamazaki et al discovered that the serum 8-OHdG levels in H 2 -treated race horses were significantly suppressed, strongly suggesting a protective effect of H 2 in exercise-induced, ROS-mediated detrimental tissue damage [ 154 ].…”

Section: Preventive and Therapeutic Applications Of H 2 mentioning

confidence: 99%

Hydrogen: A Novel Option in Human Disease Treatment

Yang

Dong

et al. 2020

Oxidative Medicine and Cellular Longevity

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H2 has shown anti-inflammatory and antioxidant ability in many clinical trials, and its application is recommended in the latest Chinese novel coronavirus pneumonia (NCP) treatment guidelines. Clinical experiments have revealed the surprising finding that H2 gas may protect the lungs and extrapulmonary organs from pathological stimuli in NCP patients. The potential mechanisms underlying the action of H2 gas are not clear. H2 gas may regulate the anti-inflammatory and antioxidant activity, mitochondrial energy metabolism, endoplasmic reticulum stress, the immune system, and cell death (apoptosis, autophagy, pyroptosis, ferroptosis, and circadian clock, among others) and has therapeutic potential for many systemic diseases. This paper reviews the basic research and the latest clinical applications of H2 gas in multiorgan system diseases to establish strategies for the clinical treatment for various diseases.

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“…16 Peng Guan et al found that H 2 improved chronic intermittent hypoxia (CIH)induced kidney injury via the JNK signaling pathway. 35 Zhao et al revealed that H 2 alleviated ER stress and apoptosis of cardiac myocytes by blocking the c-Jun N-terminal kinase-(JNK)-MAPK. 36 Wu et al uncovered that H 2 attenuated the hypoxic-ischemic brain damage by suppressing apoptosis and inflammation in neonatal rats.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen (H <sub>2</sub>) Alleviates Osteoarthritis by Inhibiting Apoptosis and Inflammation via the JNK Signaling Pathway

et al. 2020

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Background: Osteoarthritis (OA) is a very common condition and leads to joint pain, disability, and price tag all over the world. Pathogenesis of OA is closely related to numerous inflammatory and apoptosis cytokines. Hydrogen (H 2 ) reportedly exhibits a diversity of effects such as anti-apoptotic, anti-inflammatory, and anti-oxidative properties via the JNK pathway. However, it is unknown whether H 2 has a protective effect against OA via the JNK signaling pathway. Therefore, the aim of this study was to figure out whether hydrogen has protective effect on chondrocyte and further explore the possible underlying mechanism. Methods: The chondrocytes were obtained from the human cartilage tissues. Cells were stimulated by TBHP and treated with hydrogen. In vitro treatment effects were evaluated by Western blot assay, real-time PCR, immunofluorescence and TUNEL method. We conducted mice model of destabilization of the medial meniscus (DMM) and treated with hydrogen. In vivo treatment effects were evaluated by X-ray imaging assay, safranin O (SO) staining, TUNEL staining and immunohistochemical assay. Results: Our results showed that hydrogen can inhibit inflammatory factors (ADAMTS5 and MMP13) and apoptosis factors (cleaved caspase-3, cytochrome c, and Bax) in TBHPinduced chondrocytes. Furthermore, hydrogen can suppress the activation of JNK signaling pathway, whereas the effect of hydrogen can be abolished by anisomycin (a JNK activator). In vivo results showed that hydrogen can down-regulate the expression of p-JNK and cleaved caspase-3 expression. Conclusion:We uncovered that hydrogen (H 2 ) could alleviate apoptosis response and ECM degradation in human chondrocytes via inhibiting the activation of the JNK signaling pathway. Meanwhile, in the surgically-induced DMM mice model, treatment with hydrogen (H 2 ) performed a significant role in OA progression.

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Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis

Cited by 30 publications

References 46 publications

Suppression of autophagy facilitates hydrogen gas‑mediated lung cancer cell apoptosis

Suppression of autophagy facilitates hydrogen gas‑mediated lung cancer cell apoptosis

Hydrogen: A Novel Option in Human Disease Treatment

Hydrogen (H <sub>2</sub>) Alleviates Osteoarthritis by Inhibiting Apoptosis and Inflammation via the JNK Signaling Pathway

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