Hydrogen production in anaerobic and microaerobic Thermotoga neapolitana

Eriksen, Niels Thomas; Nielsen, Thomas Marius; Iversen, Niels

doi:10.1007/s10529-007-9520-5

Cited by 52 publications

(38 citation statements)

References 19 publications

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“…Microaerobic species, that require O 2 partial pressures below atmospheric levels for aerobic metabolism, have been described such as Klebisalle oxytoca HP1. 24 The question of oxygen tolerance and potential microaerobic metabolism of T. neapolitana and T. maritima has been raised in several studies 10,12,14,22,25,26 and is addressed here. Belkin et al 10 and Childers et al 22 found that it was possible to maintain cultures under aerobic conditions at temperatures below the temperature range for growth.…”

Section: V C 2009 American Institute Of Chemical Engineersmentioning

confidence: 98%

“…9 T. neapolitana is a rod-shaped, gram-negative bacterium that grows between 55 and 90 C. 10,11 T. neapolitana consumes a variety of carbohydrate substrates including ribose, xylose, glucose, sucrose, maltose, lactose, galactose, starch, and glycogen. 11 Although some of the metabolic products of T. neapolitana have been analyzed, [12][13][14][15] complete carbon and electron equivalents balances are not currently available for this species and are presented here. Such data is available for the related organism Thermotaga Correspondence concerning this article should be addressed to L. P. Walker at lpw1@cornell.edu.…”

Section: Introductionmentioning

confidence: 99%

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The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

Munro

Zinder

Walker

2009

Biotechnology Progress

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The hyperthermophilic bacterium, Thermotoga neapolitana, has potential for use in biological hydrogen (H(2)) production. The objectives of this study were to (1) determine the fermentation stoichiometry of Thermotoga neapolitana and examine H(2) production at various growth temperatures, (2) investigate the effect of oxygen (O(2)) on H(2) production, and (3) determine the cause of glucose consumption inhibition. Batch fermentation experiments were conducted at temperatures of 60, 65, 70, 77, and 85 degrees C to determine product yield coefficients and volumetric productivity rates. Yield coefficients did not show significant changes with respect to growth temperature and the rate of H(2) production reached maximum levels in both the 77 degrees C and 85 degrees C experiments. The fermentation stoichiometry for T. neapolitana at 85 degrees C was 3.8 mol H(2), 2 mol CO(2), 1.8 mol acetate, and 0.1 mol lactate produced per mol of glucose consumed. Under microaerobic conditions H(2) production did not increase when compared to anaerobic conditions, which supports other evidence in the literature that T. neapolitana does not produce H(2) through microaerobic metabolism. Glucose consumption was inhibited by a decrease in pH. When pH was adjusted with buffer addition cultures completely consumed available glucose.

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Section: V C 2009 American Institute Of Chemical Engineersmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

Munro

Zinder

Walker

2009

Biotechnology Progress

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“…As noted previously in various other studies [19], yields under thermophilic conditions tend to be higher. Thus, a thermophilic bacterium, Thermotoga neapolitana, gave a hydrogen yield of 2.4 ± 0.3 mol H 2 mol -1 glucose with acetic acid and lactic acid as additional metabolic end products [61]. For reasons that aren't clear, malonic acid addition increased H 2 yields to 3.5-3.8 mol H 2 mol -1 glucose.…”

Section: Isolation and Properties Of Novel Hydrogen Producersmentioning

confidence: 98%

Microbiological and engineering aspects of biohydrogen production

et al. 2009

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Dramatically rising oil prices and increasing awareness of the dire environmental consequences of fossil fuel use, including startling effects of climate change, are refocusing attention worldwide on the search for alternative fuels. Hydrogen is poised to become an important future energy carrier. Renewable hydrogen production is pivotal in making it a truly sustainable replacement for fossil fuels, and for realizing its full potential in reducing greenhouse gas emissions. One attractive option is to produce hydrogen through microbial fermentation. This process would use readily available wastes as well as presently unutilized bioresources, including enormous supplies of agricultural and forestry wastes. These potential energy sources are currently not well exploited, and in addition, pose environmental problems. However, fuels are relatively low value products, placing severe constraints on any production process. Therefore, means must be sought to maximize yields and rates of hydrogen production while at the same time minimizing energy and capital inputs to the bioprocess. Here we review the various attributes of the characterized hydrogen producing bacteria as well as the preparation and properties of mixed microfl ora that have been shown to convert various substrates to hydrogen. Factors affecting yields and rates are highlighted and some avenues for increasing these parameters are explored. On the engineering side, we review the potential waste pre-treatment technologies and discuss the relevant bioprocess parameters, possible reactor confi gurations, including emerging technologies, and how engineering design-directed research might provide insight into the exploitation of the signifi cant energy potential of biomass resources.

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“…Thermotoga neopolitana was first described by Jannasch and co-workers (1988) but earliest data of H 2 production is from 2002 where the bacterium produced 2.0 ml L -1 h -1 on glucose in batch cultures (van Ootegehem et al, 2002). H 2 production capacity from glucose by this species has since then been investigated in detail by others (Eriksen et al, 2008 (van Niel et al, 2002) and T. maritima (Nguyen et al, 2008;Schröder et al, 1994) with H 2 yields varying from 1.67 to 4.00 (maximum) mol H 2 mol glucose -1 . Table 3.…”

Section: Wwwintechopencommentioning

confidence: 98%