Hydrogen production by Clostridium thermolacticum during continuous fermentation of lactose

Collet, Christophe; Adler, N.; Schwitzguébel, Jean‐Paul; Péringer, Paul

doi:10.1016/j.ijhydene.2004.02.009

Cited by 179 publications

(61 citation statements)

References 32 publications

(26 reference statements)

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“…Yeast Extracts are rich in nitrogen, vitamins and other growth stimulating compounds and therefore are used as an ingredient in media for the cultivation of microorganisms C/N ratio of 70 obtained in this study was higher than 47 obtained by Lin & lay [4] who found at a C/Nratio of 47, the hydrogen yield reached 600 mL g −1 sucrose. They attributed this increased by 500%, compared with the blank to proper C/N-ratio and that lead to enhancement of hydrogen production since they used mixed culture but for the bacterium we used in this study we found that at optimum C/N ratio of 70, hydrogen yield was the maximum and reached to 350 mL g −1 glucose utilized 280 mL g [23] : Each 125 mL of H 2 1 mole H 2 , and that was higher than reported values in the literature for mesophilic species of clostridia as reported by Collet et al [24], and using glucose as substrate.…”

Section: Discussionmentioning

confidence: 74%

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Kalil¹,

Alshiyab²,

Yusoff³

2008

American J. of Biochemistry and Biotechnology

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Section: Discussionmentioning

confidence: 74%

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Kalil¹,

Alshiyab²,

Yusoff³

2008

American J. of Biochemistry and Biotechnology

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“…The H 2 yield from C. butyricum could in theory reach 4 mol H 2 /mol hexose ( Figure 2C) although a detailed metabolic analysis of C. butyricum calculated a maximum of 3.26 mol H 2 /mol hexose (Chen et al 2006) and practical yields obtained using clostridia rarely exceed 2 mol H 2 /mol hexose (Collet et al 2004;Ferchichi et al 2005). The clostridial species selected for H 2 production produce acetate and butyrate rather than propionate but they sporulate in response to environmental stresses such as heat or nutrient depletion, hence, the feeding regimes used in continuous culture are designed to maintain excessive nutrient concentrations to minimise sporulation (Hawkes et al 2002).…”

Section: Axenic Dark Fermentationsmentioning

confidence: 99%

“…have been investigated for bio-hydrogen production (Collet et al 2004), of which C. butyricum is perhaps the best known. Like E. coli and E. aerogenes, this organism is mesophilic but unlike them, it is a strict anaerobe.…”

Section: Axenic Dark Fermentationsmentioning

confidence: 99%

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Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Redwood

Paterson-Beedle

Macaskie

2008

Rev Environ Sci Biotechnol

135

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Biological methods of hydrogen production are preferable to chemical methods because of the possibility to use sunlight, CO 2 and organic wastes as substrates for environmentally benign conversions, under moderate conditions. By combining different microorganisms with different capabilities, the individual strengths of each may be exploited and their weaknesses overcome. Mechanisms of bio-hydrogen production are described and strategies for their integration are discussed. Dual systems can be divided broadly into wholly light-driven systems (with microalgae/cyanobacteria as the 1 st stage) and partially light-driven systems (with a dark, fermentative initial reaction). Review and evaluation of published data suggests that the latter type of system holds greater promise for industrial application. This is because the calculated land area required for a wholly light-driven dual system would be too large for either centralised (macro-) or decentralised (micro-)energy generation. The potential contribution to the hydrogen economy of partially light-driven dual systems is overviewed alongside that of other bio-fuels such as bio-methane and bio-ethanol.Redwood et al. -Dual systems for Bio-H 2 -Reviews in Environ. Sci. Bio/Technol. 8:149 http://dx

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“…Clostridia, Enterobacter and Escherichia have been investigated for hydrogen production by many authors [8][9][10][11]. Bacterial strains used in the present work as hydrogen producers (except for E. coli BW25113 and E. coli MSCL 332) have been isolated in Latvia and the goal from this and further investigations is to use crude glycerol as a substrate from Latvia's biodiesel production leftovers.…”

Section: Introductionmentioning

confidence: 99%

Assessment of bio-hydrogen production from glycerol and glucose by fermentative bacteria

Dimanta¹,

Nikolajeva²,

Gruduls³

et al. 2013

Energetika

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Microorganisms are capable to produce hydrogen during fermentation of organic substrates and industrial waste products can be used as feedstock for hydrogen producing bacteria. One of the substrates that can be effectively used for microbial hydrogen production is glycerol, which is a by-product from the process of biodiesel production, but glucose is mainly used as a model substrate. Different bacterial isolates were tested for hydrogen gas production rates from glucose and glycerol with test-systems constructed in our laboratory. Test-systems were optimised to allow adequate substrate and bacterial strain hydrogen productivity estimation in the liquid and gaseous phases. It was concluded that several of the isolated bacterial strains are suitable for bio-hydrogen production using glycerol as a substrate. Assessment was developed to establish whether microbial conversion of glycerol is an economically and environmentally viable possibility for bio-hydrogen production. The raw material cost noticeably decreases because of large quantities of available crude glycerol after biodiesel production and the highly reduced nature of carbon in glycerol per se.Key words: bio-hydrogen, fermentation, substrates, prototype bioreactor INTRODUCTIONBiological production of hydrogen using bacteria is a promising and advantageous area, especially when hydrogen is gained from a variety of renewable resources [1,2]. Industrial and agricultural organic waste used as feedstock for hydrogen producing bacteria is a perspective way for alternative energy production and it noticeably decreases the raw material cost. During the conversion of organic wastes, in anaerobic environment, hydrogen gas is produced as a by-product. Substantial factors like availability and cost are highly important in the selection of waste materials to be used in hydrogen production with fermentative bacteria [3]. One of the substrates that can be effectively used for microbial hydrogen production is glycerol, which is a by-product from the process of biodiesel production. Because of large quantities available of crude glycerol and the highly reduced nature of carbon in glycerol per se, microbial conversion of it seems to be economically and environmentally viable possibility, 125Assessment of bio-hydrogen production from glycerol and glucose by fermentative bacteria especially because over the last several years the demand and production of biodiesel has remarkably increased [4,5]. Recently several authors have investigated hydrogen production using glycerol as a substrate by fermentative bacteria. Mangayil et al. [6] investigated optimal conditions (pH 6.5; 40 °C and 1 g/L raw glycerol) for hydrogen production using crude glycerol with microbial consortium mainly dominated by Clostridium species. Environmental conditions like medium pH and temperature are the major parameters to be controlled in the hydrogen production because they affect the qualitative and quantitative content of bacterial produced gas and the hydrogen yield and rate. Hydrogen production usi...

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Hydrogen production by Clostridium thermolacticum during continuous fermentation of lactose

Cited by 179 publications

References 32 publications

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Integrating dark and light bio-hydrogen production strategies: towards the hydrogen economy

Assessment of bio-hydrogen production from glycerol and glucose by fermentative bacteria

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