Hydrogen peroxide oxidation of o-vanillin fluorescence kinetic fluorescence method for the determination of Mn(II)Hydrogen peroxide oxidation of o-vanillin fluorescence kinetic fluorescence method for the determination of Mn(II)

Abstract

In this experiment, a newly synthesized chromogenic agent o-vanillin fluorescence ketone was used to establish a method for the determination of Mn(II) by catalytic kinetic fluorescence. At the maximum excitation and emission wavelengths (λex/λem = 495 nm/578 nm) Mn(II) showed a negative correlation with fluorescence intensity in the range of 0.005 ~ 0.025 µg/mL and a good linear relationship between concentration and ΔF value. The optimal experimental conditions were investigated and the possible reaction mechanism was explored. The linear regression equation was ΔF = 937.7714 + 7287.7498ρ (µg/mL), r = 0.9993. The detection limit was 3.690×10− 3 µg/mL. The samples were analyzed after masking the interfering ions, and trace amounts of Mn(II) were detected in tap water, Yellow River water and tea leachate with the spiked recoveries of 99.05%, 100.0% and 103.32%, respectively. Satisfactory results were obtained.