Hydrogen peroxide mediates the expression of ascorbate-related genes in response to methanol stimulation in Oncidium

Ectopic expression of FaGalUR leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato

“…PCR amplification included a 5-min pre-incubation step at 95°C, followed by 40 cycles of 95°C for 10 s, 58°C for 15 s and 72°C for 20 s. PCR products were quantified by the LightCycler 480 Real-Time PCR Detection System with the SYBR Green I Master Kit (Roche). D-galacturonic acid reductase (GalUR) activity assay Protein extraction from wild-type and southern positive T 1 lines was carried out as described (Shen and Yeh 2010). One gram of samples were homogenized in liquid nitrogen and extracted with 50 mM sodium phosphate buffer, pH7.2, containing 2 mM EDTA, 2 mM DTT, 20 % glycerol and PVPP.…”

Deciphering the molecular responses to methanol-enhanced photosynthesis and stomatal conductance in broad bean

“…In oncidium PLB treated with 50 mM methanol, alcohol oxidase is activated, thereby leading to methanol oxidation and the generation of H 2 O 2 and formaldehyde (Shen and Yeh 2010). Thus, the oxidation of methanol might increase H 2 O 2 content in plants, which could induce the expression of antioxidant enzymes in plants.…”

“…Methanol treatment induces the expression of a specific PME gene in Arabidopsis and tomato (Ramírez et al 2006). The treatment of Oncidium protocorm-like bodies (PLB) with 50 mM methanol effectively enhanced the expression of ascorbate (ASA)-biosynthetic genes in the Smirnoff-Wheeler and galacturonate (GalUA) pathways (Shen and Yeh 2010). Treatment of Arabidopsis with 5 mM methanol induced the expression of many photosynthesis-related genes, including Rubisco activase (RCA), the photosystem II chlorophyll-binding protein (LHC-II) Lhcb3, and NADPdependent glyceraldehyde-3-phosphate dehydrogenase (GADPH), but inhibited the expression of the LHC-II gene Lhcb2 (Unpublished results).…”

Stimulation of accumulation of lipid bodies in Chlamydomonas reinhardtii cells

“…супроводжується підвищенням вмісту ліпідів [1]. Утилізація метанолу пов'язана зі зростанням внутрішньоклітинної концентрації Н 2 О 2 [7]. Існують відомос-ті, що окисний стрес, обумовлений екзогенним Н 2 О 2 , зумовлює накопичення ліпідів у C. reinhardtii [10].…”