Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway

Orozco-Cárdenas, Martha L.; Ryan, Clarence A.

doi:10.1073/pnas.96.11.6553

Cited by 736 publications

(470 citation statements)

References 34 publications

(43 reference statements)

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“…H 2 O 2 stimulates SA and ethylene production, induces PR protein expression, and enhances pathogen tolerance (Wu et al, 1997;Chamnongpol et al, 1998). Systemic H 2 O 2 production has also been observed in response to wounding in several plant species including barley and maize (Orozco-Cardenas and Ryan, 1999), and H 2 O 2 has been confirmed as a second messenger in activating defense gene expression (Orozco-Cardenas et al, 2001). Accumulation of H 2 O 2 is triggered by oligoGalUA released from the cell walls of vascular bundle cells by wound-induced plant polygalacturonases (Bergey et al, 1999).…”

Section: H 2 O 2 May Play a Role In Plant Defense Against Aphidsmentioning

confidence: 99%

“…Production of ROS, particularly H 2 O 2 , has repeatedly been associated with diverse plant-pathogen and plant-insect interactions (Alvarez et al, 1998;Orozco-Cardenas and Ryan, 1999;Orozco-Cardenas et al, 2001). H 2 O 2 stimulates SA and ethylene production, induces PR protein expression, and enhances pathogen tolerance (Wu et al, 1997;Chamnongpol et al, 1998).…”

Section: H 2 O 2 May Play a Role In Plant Defense Against Aphidsmentioning

confidence: 99%

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Transcriptional Regulation of Sorghum Defense Determinants against a Phloem-Feeding Aphid

et al. 2004

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When attacked by a phloem-feeding greenbug aphid (Schizaphis graminum), sorghum (Sorghum bicolor) activates jasmonic acid (JA)- and salicylic acid (SA)-regulated genes, as well as genes outside known wounding and SA signaling pathways. A collection of 672 cDNAs was obtained by differential subtraction with cDNAs prepared from sorghum seedlings infested by greenbug aphids and those from uninfested seedlings. Subsequent expression profiling using DNA microarray and northern-blot analyses identified 82 transcript types from this collection responsive to greenbug feeding, methyl jasmonate (MeJA), or SA application. DNA sequencing analyses indicated that these encoded proteins functioning in direct defense, defense signaling, oxidative burst, secondary metabolism, abiotic stress, cell maintenance, and photosynthesis, as well as proteins of unknown function. In response to insect feeding, sorghum increased transcript abundance of numerous defense genes, with some SA-dependent pathogenesis-related genes responding to greenbug more strongly than to SA. In contrast, only weak induction of MeJA-regulated defense genes was observed after greenbug treatment. However, infestation tests confirmed that JA-regulated pathways were effective in plant defense against greenbugs. Activation of certain transcripts exclusively by greenbug infestation was observed, and may represent unique signal transduction events independent of JA- and SA-regulated pathways. Results indicate that plants coordinately regulate defense gene expression when attacked by phloem-feeding aphids, but also suggest that aphids are able to avoid triggering activation of some otherwise potentially effective plant defensive machinery, possibly through their particular mode of feeding.

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Section: H 2 O 2 May Play a Role In Plant Defense Against Aphidsmentioning

confidence: 99%

Section: H 2 O 2 May Play a Role In Plant Defense Against Aphidsmentioning

confidence: 99%

Transcriptional Regulation of Sorghum Defense Determinants against a Phloem-Feeding Aphid

et al. 2004

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“…Chlorophyll was extracted for 10 min with boiling ethanol and 2 h with ethanol at room temperature before photography (Orozco-Cardenas and Ryan, 1999).…”

Section: Histochemical Analysesmentioning

confidence: 99%

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

et al. 2015

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The structure of the cell wall has a major impact on plant growth and development, and alteration of cell wall structural components is often detrimental to biomass production. However, the molecular mechanisms responsible for these negative effects are largely unknown. Arabidopsis (Arabidopsis thaliana) plants with altered pectin composition because of either the expression of the Aspergillus niger polygalacturonase II (AnPGII; 35S:AnPGII plants) or a mutation in the QUASIMODO2 (QUA2) gene that encodes a putative pectin methyltransferase (qua2-1 plants), display severe growth defects. Here, we show that expression of Arabidopsis PEROXIDASE71 (AtPRX71), encoding a class III peroxidase, strongly increases in 35S:AnPGII and qua2-1 plants as well as in response to treatments with the cellulose synthase inhibitor isoxaben, which also impairs cell wall integrity. Analysis of atprx71 loss-of-function mutants and plants overexpressing AtPRX71 indicates that this gene negatively influences Arabidopsis growth at different stages of development, likely limiting cell expansion. The atprx71-1 mutation partially suppresses the dwarf phenotype of qua2-1, suggesting that AtPRX71 contributes to the growth defects observed in plants undergoing cell wall damage. Furthermore, AtPRX71 seems to promote the production of reactive oxygen species in qua2-1 plants as well as plants treated with isoxaben. We propose that AtPRX71 contributes to strengthen cell walls, therefore restricting cell expansion, during normal growth and in response to cell wall damage.

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“…This type of assay has been commonly employed as a marker for stress response in higher plants (Orozco-Cardenas and Ryan, 1999) and marine algae (Collen and Pedersen, 1994;Küpper et al, 2002;Ross et al, 2005). The response is based on the reaction of hydrogen peroxide with the fluorogenic probe dichlorofluorescein diacetate (DCFH-DA, Invitrogen Corp., Carlsbad, CA, USA).…”

Section: Measurements Of Cellular Stressmentioning

confidence: 99%

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

2006

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Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway

Abstract: Hydrogen peroxide (H 2 O 2 ) generated in response to wounding can be detected at wound sites and in distal leaf veins within 1 hr after wounding. The response is systemic and maximizes at about 4-6 hr in both wounded and unwounded leaves, and then declines.

Cited by 736 publications

References 34 publications

Transcriptional Regulation of Sorghum Defense Determinants against a Phloem-Feeding Aphid

Transcriptional Regulation of Sorghum Defense Determinants against a Phloem-Feeding Aphid

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

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