Hydrogen peroxide induces cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo

Abstract

The concentration of HO influences effects on the pulp tissue, where a higher concentration of HO can cause necrosis in the pulp and a prolonged effect within the apoptotic process; lower concentrations of HO provide moderate inflammation, cell proliferation and apoptosis with a reduction of these processes over time.

Concentration‐dependent effect of bleaching agents on the immunolabelling of interleukin‐6, interleukin‐17 and CD5‐positive cells in the dental pulp

“…Similar results were obtained previously with a lower H 2 O 2 concentration that resulted in the greater presence of apoptotic cells than higher concentration that resulted in the presence of necrosis (Benetti et al . ). However, these results disagree with those observed with the immunolabelling of IL‐6 and IL‐17, where the intensity was proportional to H 2 O 2 concentration in bleaching gels.…”

“…, Benetti et al . ). The values of total central area of the pulp chamber were also obtained, and by taking into account the values obtained, it was possible to calculate the reduction percentage in the central area of the pulp chamber in the treated groups in comparison with the central area of the control group.…”

“…To analyse CD5, cells that had brown cytoplasm were counted (Benetti et al . ), and the area of each third of pulp chamber was measured in each specimen by image processing software (Leica QWin V3, Leica Microsystems), to subsequently express the number of cells per mm 2 (Cintra et al . , Benetti et al .…”

“…, , Benetti et al . ). This would explain why most patients who undergo this treatment have tooth sensitivity (Charakorn et al .…”

“…, Benetti et al . ,b). Also, cytokines have been shown to be involved in acute inflammation 6 h after dental bleaching (Soares et al .…”