Hydrogen peroxide induced stress in human keratinocytes and its effect on bithionol toxicity

Reid, Linzi; Clothier, Richard H.; Khammo, N.

doi:10.1016/s0887-2333(01)00049-2

Cited by 18 publications

(14 citation statements)

References 13 publications

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“…In the in vivo study (Fig 2), topical application of L-741626 significantly enhanced epidermal hyperplasia induced by barrier disruption. This in vivo effect of L-741626 might be due to elongation of the delay in barrier recovery, as shown in Fig 1. The results of Alamar Blue assay are shown in Fig 7B. The levels of fluorescence indicate the activity of mitochondria, which is considered to be a measure of the viability of the cells (Reid et al, 2001). In this study, the viability closely paralleled the cell number, indicating that the reduction of keratinocyte proliferation by bromocriptine was not due to cytotoxicity of the reagent.…”

Section: Figuresupporting

confidence: 55%

“…Alamar blue assay To evaluate the cytotoxicity of bromocriptine and L-741626, we carried out Alamar blue assay (Reid et al, 2001). First, we seeded 3x10 4 keratinocytes per well in 12-well plates with 2 mL of KG2 medium (Kurabo) and incubated them for 48 h. Then, the medium was changed and bromocriptine (final concentration 10 nM) or L-741626 (final concentration 10 nM) was applied.…”

Section: Methodsmentioning

confidence: 99%

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Dopamine D2-Like Receptor Agonists Accelerate Barrier Repair and Inhibit the Epidermal Hyperplasia Induced by Barrier Disruption

Fuziwara

Suzuki

Inoué

et al. 2005

Journal of Investigative Dermatology

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Two families of G protein-coupled receptors of the neurotransmitter dopamine, i.e., dopamine 1-like family (D1-like receptor) and dopamine 2-like family (D2-like receptor), show dopaminergic activity in nerve cells. The D2-like receptor family, composed of D2, D3, and D4 receptors, downregulates the intracellular cAMP signaling pathway, and dopamine receptor agonists reduce the cAMP level in neurons. We previously demonstrated that the cAMP level in epidermal keratinocytes is related to epidermal barrier homeostasis. Since keratinocytes are known to carry various neurotransmitter receptors, we hypothesized that D2-like receptors on keratinocytes might be related to skin barrier homeostasis. In this study, we examined the effect of topical application of receptor agonists and antagonists on skin barrier recovery after barrier disruption. Application of D2-like receptor agonists accelerated barrier recovery, whereas D2-like receptor antagonists delayed it. D2-like receptor agonists also reduced the epidermal hyperplasia induced by barrier disruption under low environmental humidity. Immunohistochemical study and RT-PCR analysis revealed the expression of the D2 receptor in the basal epidermis and the D4 receptor in the uppermost layer of the epidermis. These results suggest that dopaminergic receptors are involved in epidermal barrier homeostasis.

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Section: Figuresupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Dopamine D2-Like Receptor Agonists Accelerate Barrier Repair and Inhibit the Epidermal Hyperplasia Induced by Barrier Disruption

Fuziwara

Suzuki

Inoué

et al. 2005

Journal of Investigative Dermatology

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“…The phenomenon of photo-oxidation might have caused the impairment of metabolic activity in the cells. The Alamar blue assay, which is non-radioactive, has been used in in vitro photocytotoxicity studies of human RPE, cultured ocular lenses (bovine and porcine), mouse neural crest cells, and human keratinocytes [18,36,[38][39][40][41]. Findings from these studies support the use of the Alamar blue as a sensitive assay for monitoring UV-induced changes in intracellular mitochondrial, cytoplasmic and nuclear integrity, all of which are measures of cellular viability [36].…”

Section: Discussionmentioning

confidence: 87%

Phototoxicity of Ultraviolet (UV) Radiation: Evaluation of UV-Blocking Efficiency of Intraocular Lens (IOL) Materials Using Retinal Cell Culture and in vitro Bioassays~!2009-06-08~!2010-02-10~!2010-03-26~!

Youn¹,

Cullen²,

Chou³

et al. 2010

TOTOXIJ

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This work involves the evaluation of UV blocking efficiency of commercially available intraocular lens (IOL) materials using a retinal cell culture and a biological in vitro model that was developed in a previous study, as an effort to examine the sensitivity of this in vitro approach for evaluating toxicity of UV radiation on the retinal pigment epithelial cells. The human retinal pigment epithelial (RPE) cell line, ARPE-19, was cultured, and cells were irradiated with broadband UVB radiations at energy levels of 0.2 and 0.4 J/cm. Some treated cells were not shielded from the radiation while others were shielded using two thicknesses (0.9 and 1.5 mm) of IOL material. After irradiation, cellular viability, mitochondrial distribution, nuclei morphology, and phagocytotic activity were analyzed using the Alamar blue assay, Rhodamine 123 staining, the Hoechst assay, and a phagocytotic activity assay. The results demonstrate that UVB radiation can cause significant decreases in RPE cell viability as well as in phagocytotic activity. Also, the results show that UVB radiation can induce the degradation of DNA and mitochondria in cultured RPE cells. However, the two different thickness IOL material sheets (0.9 and 1.5 mm) showed very effective UV blocking ability, allowing no cellular damage at all. Thus, the finding suggest that these four assays together can be used as a sensitive, and meaningful in vitro biomarker method for evaluating toxicity of UV radiation on RPE cells, and also for examining IOL effectiveness.

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“…The method employed was similar to that described previously [13,14]. Briefly, a working solution of 2 0 ,7 0 -dichlorofluorescin diacetate (DCFH-DA) in PBS was prepared prior to use and 500 ll well -1 added to each experimental well containing cells grown for 3 days, plus a blank column of wells for reference.…”

Section: Oxidative Stress Assaymentioning

confidence: 99%

Plasma-induced nanopillars on bare metal coronary stent surface for enhanced endothelialization

et al. 2010

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Hydrogen peroxide induced stress in human keratinocytes and its effect on bithionol toxicity

Cited by 18 publications

References 13 publications

Dopamine D2-Like Receptor Agonists Accelerate Barrier Repair and Inhibit the Epidermal Hyperplasia Induced by Barrier Disruption

Dopamine D2-Like Receptor Agonists Accelerate Barrier Repair and Inhibit the Epidermal Hyperplasia Induced by Barrier Disruption

Phototoxicity of Ultraviolet (UV) Radiation: Evaluation of UV-Blocking Efficiency of Intraocular Lens (IOL) Materials Using Retinal Cell Culture and in vitro Bioassays~!2009-06-08~!2010-02-10~!2010-03-26~!

Plasma-induced nanopillars on bare metal coronary stent surface for enhanced endothelialization

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