Hydrogen peroxide determination in pharmaceutical formulations and cosmetics using a new catalase biosensor

Campanella, Luigí; Roversi, R; Sammartino, Maria Pia; Tomassetti, M.

doi:10.1016/s0731-7085(98)00155-1

Cited by 85 publications

(43 citation statements)

References 14 publications

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“…for food [9], pharmaceutical [10], environmental analysis [11], medicine [12]). An important biocompound that is in need of reliable monitoring is l-lactate.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Taurino

Reiss

Richter

et al. 2013

Electrochimica Acta

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Keywords:l-Lactate oxidases from Aerococcus viridans Engineered and commercial enzymes Multi-walled carbon nanotubes Enzyme biosensor a b s t r a c t A comparison between engineered and commercially available l-lactate oxidases from Aerococcus viridans was conducted for biosensing applications. Enzymes were adsorbed onto the surfaces of graphite electrodes modified with multi-walled carbon nanotubes. Thermostable l-lactate oxidases were cloned with a (i) N-, (ii) a C-terminal His-tag and (iii) a wild-type enzyme. Subsequently to the heterologous expression in Escherichia coli and purification, we determined the kinetic parameters of these enzymes in solution. The kinetics of the wild-type, of the N-terminally His-tagged enzyme and of the commercial l-lactate oxidase from A. viridans were studied with a classical Michaelis-Menten as well as with a substrate inhibition model, while the enzyme carrying a C-terminal His-tag showed no activity. The active enzymes were used to fabricate and comparatively investigate multi-walled carbon nanotubes-based biosensors. The enzyme kinetic results were compared with electrochemical studies. By using both spectrophotometric and amperometric techniques, the inhibition phenomenon fits better to the data especially those data related with Lox-His-N. The electrochemical data of the fabricated enzymatic biosensors showed that the N-terminally His-tagged l-lactate oxidase performed best on carboxyl-modified carbon nanotubes. The sensor based on this engineered enzyme showed the highest sensitivity and lowest detection limit in the range of l-lactate concentration 0-1 mM as well as long term stability over one month.

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“…for food [9], pharmaceutical [10], environmental analysis [11], medicine [12]). An important biocompound that is in need of reliable monitoring is l-lactate.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Taurino

Reiss

Richter

et al. 2013

Electrochimica Acta

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“…In aqueous solutions and in presence of various cations, κ-carrageenan forms thermoreversible gels on cooling. It is extensively used as thickening, gelling agent, texture enhancer or stabilizer in the food, pharmaceutical and cosmetic industries [4][5][6]. More recently drug delivery systems based on κ-carrageenan gels were tested in vitro [7][8].…”

Section: Introductionmentioning

confidence: 99%

Relation between structural and release properties in a polysaccharide gel system

Mangione

Giacomazza

Cavallaro

et al. 2007

Biophysical Chemistry

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The potential utility of κ-carrageenan gels for preparing drug release devices is here shown. Structural properties of κ-carrageenan gels prepared with different salt composition and containing Ketoprofen sodium salt, as model drug, have been evaluated with static light scattering and rheological measurements. These properties have been correlated with release profiles in vitro at pH 5.5. Release properties from gelled matrices have been compared with those obtained by two commercial products containing the same drug. Results show that: i) in this system it is possible to easily control the gel texture by using different cationic concentration; ii) the kinetics of drug release by κ-carrageenan gels are dependent on the structural properties of matrices; iii) in the typical interval time used in classical local applications, all gel samples release the loaded drug almost completely, at difference with the commercial products. All these findings can provide useful suggestions for the realization of classical topical release systems.

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“…[11][12][13][14] Many analytical techniques are available to determine H2O2, including fluorometry, 15 chemiluminescence, 16 photometry 17 and voltammetry. 18 Among applications, the determination of hydrogen peroxide has been realized in matrices such as foods [19][20][21] and pharmaceutics [22][23][24] and in the environment. [25][26][27] In recent years, one of the most widely used electroanalytical methods relies on the use of peroxidase.…”

mentioning

confidence: 99%

Sensor for Hydrogen Peroxide Based on Prussian Blue Modified Electrode: Improvement of the Operational Stability

et al. 2000

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Hydrogen peroxide determination in pharmaceutical formulations and cosmetics using a new catalase biosensor

Cited by 85 publications

References 14 publications

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Relation between structural and release properties in a polysaccharide gel system

Sensor for Hydrogen Peroxide Based on Prussian Blue Modified Electrode: Improvement of the Operational Stability

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