Hydrogen peroxide can be a plausible biomarker in cyanobacterial bloom treatment

Abstract

The effect of combined stresses, photoinhibition, and nutrient depletion on the oxidative stress of cyanobacteria was measured in laboratory experiments to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous (P) concentrations with fixed nitrogen concentrations. The samples were subjected to stress assays by detecting the hydrogen peroxide (H2O2) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H2O2 concentrations decreased to 30 µmol m−2 s−1 of PAR, then increased with higher PAR intensities. Regarding P concentrations, H2O2 concentrations (nmol L−1) generally decreased with increasing P concentrations. SOD and CAT activities were proportionate to the H2O2 protein−1. No H2O2 concentrations detected outside cells indicated the biological production of H2O2, and the accumulated H2O2 concentration inside cells was parameterized with H2O2 concentration protein−1. With over 30 µmol m−2 s−1 of PAR, H2O2 concentration protein−1 had a similar increasing trend with PAR intensity, independently of P concentration. Meanwhile, with increasing P concentration, H2O2 protein−1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with gradually increasing H2O2 up to 4 nmol H2O2 mg−1 protein, which provides a threshold to restrict the growth of cyanobacteria. With these results, an empirical formula—protein (mg L−1) = − 192*Log((H2O2/protein)/4.1), where H2O2/protein (nmol mg−1) = − 0.312*PAR2/(502 + PAR2)*((25/PAR)4 + 1)*Log(P/133,100), as a function of total phosphorus concentration, P (µg L−1)—was developed to obtain the cyanobacteria biomass.

Physiological and Molecular Modulations to Drought Stress in the Brassica Species

“…Hydrogen peroxide is the key signaling molecule in plants due to its relatively long half-life (on scales of seconds to minutes) and its cellular roles, such as oxidizing proteins and regulating protein function. In addition, the simple measurement method of hydrogen peroxide enabled this molecule to be used in stress monitoring in plants [ 93 , 94 , 95 , 96 ], as carried out in this study. In contrast, superoxide has an extremely short half-life (1–4 µs); thus, it cannot accumulate in cells.…”

Presentations at the 2022 MELiSSA Conference – Current and Future Ways to Closed Life Support Systems

“…It was also shown previously, that the Different letters (a-f) indicate significant differences (One-way ANOVA followed by Tukey test, p < 0,05). (Asaeda et al, 2022). Muhetaer et al (2020) suggest that the production of H 2 O 2 by the photosytem is the main reason for the reduced growth under high light in the cyanobacterial species Pseudanabaena galeata and Microcystis aeruginosa.…”

Measurement of foliar H2O2 concentration can be an indicator of riparian vegetation management

“…H 2 O 2 has been extensively used to quantify ROS damage or stress levels in many plant studies. Therefore, H 2 O 2 can be used as an indicator of the physiological status of plants and to monitor the response of plants against the intensity of environmental stress 43 – 46 . The application of stress response biomarkers, such as H 2 O 2 content in plant tissues using an empirical model, could be an efficient tool in the context of habitat suitability, too.…”