Hydrogen peroxide and salinity stress act synergistically to enhance lipids production in microalga by regulating reactive oxygen species and calcium

Qiao, Tengsheng; Zhao, Yongteng; Zhong, Du-bo; Yu, Xuya

doi:10.1016/j.algal.2020.102017

Cited by 72 publications

(35 citation statements)

References 54 publications

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“…exposed to varying H 2 O 2 concentrations (0.5 to 4 mM) [29]. The model species Phaeodactylum tricornutum also showed a significative reduction in biomass from exposure to 0.25 to 2 mM of H 2 O 2 , which has been suggested as a potential trigger of apoptosis in diatoms [26,40]. Another study demonstrated for the ochrophyte Aureococcus anophagefferens that cell size is a key factor to H 2 O 2 sensitivity, which involves reactions with a wide range of cellular organic compounds such as alcohols, esters or aromatics [39].…”

Section: Discussionmentioning

confidence: 89%

“…Other studies have documented the effects of H 2 O 2 on lipids in microalgae. For example, the content of neutral lipids, both at early and late stationary phases, was enhanced by ~ 30% (up to 96 mg/L) in P. tricornutum exposed to 0.25 mM H 2 O 2 [26]. In this context, Qiao et al [26] recently highlighted that the combination of NaCl and H 2 O 2 stress in the freshwater Monoraphidium sp.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the content of neutral lipids, both at early and late stationary phases, was enhanced by ~ 30% (up to 96 mg/L) in P. tricornutum exposed to 0.25 mM H 2 O 2 [26]. In this context, Qiao et al [26] recently highlighted that the combination of NaCl and H 2 O 2 stress in the freshwater Monoraphidium sp. can positively influence the expression of the lipogenesis regulator genes accD, KASIII and DGAT1.…”

Section: Discussionmentioning

confidence: 99%

“…This may be alleviated via the activation of metabolic pathways leading to increasing antioxidant capacity and the accumulation of bioactive compounds [22]. Compared to nutrient concentration manipulation, hydrogen peroxide (H 2 O 2 ) has seldom been considered as a stressor to modulate the homeostasis of microalgae and to influence the production of metabolites, focusing mostly a small number of diatoms and chlorophytes [23][24][25][26]. As such, the present study aimed at assessing the physiological responses of marine microalgae species selected from different lineages to H 2 O 2 -induced oxidative stress in terms of antioxidant activity and proximal biochemical composition as well as the synthesis of high-value pigments and PUFAs.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Barone

Parkes

Herbert

et al. 2021

Appl Biochem Biotechnol

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There have been growing interests in the biorefining of bioactive compounds from marine microalgae, including pigments, omega-3 fatty acids or antioxidants for use in the nutraceutical and cosmetic sectors. This study focused on the comparative responses of five marine microalgal species from different lineages, including the dinoflagellate Amphidinium carterae, chlorophyte Brachiomonas submarina, diatom Stauroneis sp., haptophyte Diacronema sp. and rhodophyte Rhodella violacea, to exposure during their batch growth to hydrogen peroxide (H 2 O 2 ). A. carterae returned an enhanced signal with the DPPH assay (8.8 µmol Trolox eq/g DW) when exposed to H 2 O 2 , which was associated with reduced pigment yields and increased proportions in saturated C16 and C18 fatty acids. B. submarina showed enhanced antioxidant response upon exposure to H 2 O 2 with the DPPH assay (10 µmol Trolox eq/g DW), a threefold decrease in lutein (from 2.3 to 0.8 mg/g) but a twofold increase in chlorophyll b (up to 30.0 mg/g). Stauroneis sp. showed a downward response for the antioxidant assays, but its pigment yields did not vary significantly from the control. Diacronema sp. showed reduced antioxidant response and fucoxanthin content (from 4.0 to 0.2 mg/g) when exposed to 0.5 mM H 2 O 2 . R. violacea exposed to H 2 O 2 returned enhanced antioxidant activity and proportions of EPA but was not significantly impacted in terms of pigment content. Results indicate that H 2 O 2 can be used to induce stress and initiate metabolic changes in microalgae. The responses were however speciesspecific, which would require further dosage optimisation to modulate the yields of specific metabolites in individual species. Keywords Microalgae • Hydrogen peroxide • Antioxidants • Pigments • FAMEs Research Highlights• Comparative response to H 2 O 2 treatment assessed for 5 microalgal species from different lineages.• Results showed an enhanced antioxidant response for Rhodella violacea and an overall reduction for the other species. • Pigment yield reduction was observed for most species except for chlorophyll b, which was enhanced in Brachiomonas submarina.• Species-specific variations in the proportions of fatty acids were observed.• Principal component analysis clearly separated the H 2 O 2 -treated Rhodella violacea set from the others, with higher antioxidant response and proportions of EPA.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Barone

Parkes

Herbert

et al. 2021

Appl Biochem Biotechnol

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“…Zhao et al (2021) show that saline stress is a device widely used and researched to maximize the synthesis of lipids in microalgae, since they are more viable processes, economically speaking. Qiao et al (2021) also highlight that causing salt stress directly affects physiological and biochemical mechanisms linked to cell growth and consequent biomass production, causing ionic, osmotic and oxidative stress. Ionic and osmotic changes caused by salt stress reduce the activity of the Na + /H + ion, which impacts on photosynthetic activity.…”

Section: Introductionmentioning

confidence: 99%

Statistical study of growth kinetics and lipid content of microalgae grown in brackish waters for bioenergetic purposes

Guimarães¹,

França²

2021

Rev. ambiente água

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This study aimed to statistically prove the influence of salinity on growth kinetics and intracellular lipid accumulation in microalgae. The species Chlorella sp., Scenedesmus acuminatus, Nannochloropsis sp., Monoraphidium contortum and Pediastrum tetras were studied, which were isolated in the Brazilian semi-arid region and cultivated in synthetic media with the addition of NaCl. From the crops, growth kinetics, dry biomass production and lipid content were analyzed, using the experimental planning for one factor as a tool, with the NaCl concentration as an independent variable. The kinetic parameters maximum growth speed (µmax) and generation time (tg), as well as lipid concentration were the response variables studied. The results showed that, in the species Scenedesmus acuminatus, Nannochloropsis sp. and Pediastrum tetras, salt stress contributed to the increase in µmax and the consequent decrease in tg. The highest levels of lipid accumulation were obtained in Nannochloropsis sp. (62.04%), in the medium with the highest salinity and Pediastrum tetras (54.04%) in the lowest. Dry biomass production was higher in Scenedesmus acuminatus (1,941.37 mg.L-1) and Nannochloropsis sp. (1237.05 mg.L-1), both at a concentration of 4.0 g.L-1. Therefore, saline stress acted directly on the cell growth and lipid content of the species, which can be used as a device to enhance lipid production for the purpose of producing biofuels.

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Chlorella vulgaris growth, pigment and lipid accumulation: effect of progressive light and hydrogen peroxide exposure

Cunha

Sátiro

Escobar

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Chlorella vulgaris is being explored for several applications such as biodiesel production, nutrient supplementation, animal feed and pharmaceutical products. In this study, the performance of a batch culture under progressive light intensity and the effect of hydrogen peroxide (H2O2) concentration on lipid accumulation were investigated. RESULTS Under progressive light intensity (60 to 300 μmol m−2 s−1), a 43% biomass increase was achieved in comparison with the control light conditions (60 μmol m−2 s−1), whereas a 300 μmol m−2 s−1 light intensity caused growth inhibition to the culture. In addition, an increase in carotenoid content from 0.102 mg g−1 biomass (control) to 0.460 mg g−1 (progressive light) and lipid accumulation from 31.0 mg g−1 (control) to 36.5 mg g−1 (progressive light) was observed. However, the moderate illumination conditions (60 μmol m−2 s−1) induced a very significant increase of chlorophyll b, regarding the progressive light mode, increasing the photosynthetic efficiency. After the growth phase, the biomass was subjected to stress conditions by adding H2O2 to the culture medium at different concentrations: 0.75 mmol L−1 increased the lipid content of the biomass from 3.6% to 10.3% in a 3‐day test, reaching lipid productivity of 70.4 mg L−1 day−1, an increase of ≈40% compared to the phosphate starvatiod for the same culture time. CONCLUSION The progressive light system allowed an increase in biomass and lipid accumulation. Together with the exposure to H2O2, this work provides a new approach for lipid and pigment production from microalgae. © 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Hydrogen peroxide and salinity stress act synergistically to enhance lipids production in microalga by regulating reactive oxygen species and calcium

Cited by 72 publications

References 54 publications

Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Statistical study of growth kinetics and lipid content of microalgae grown in brackish waters for bioenergetic purposes

Chlorella vulgaris growth, pigment and lipid accumulation: effect of progressive light and hydrogen peroxide exposure

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