Hydrogen peroxide and phosphoinositide metabolites synergistically regulate a cation current to influence neuroendocrine cell bursting

Abstract

In various neurons, including neuroendocrine cells, non‐selective cation channels elicit plateau potentials and persistent firing. Reproduction in the marine snail Aplysia californica is initiated when the neuroendocrine bag cell neurons undergo an afterdischarge, that is, a prolonged period of enhanced excitability and spiking during which egg‐laying hormone is released into the blood. The afterdischarge is associated with both the production of hydrogen peroxide (H2O2) and activation of phospholipase C (PLC), which hydrolyses phosphatidylinositol‐4,5‐bisphosphate into diacylglycerol (DAG) and inositol trisphosphate (IP3). We previously demonstrated that H2O2 gates a voltage‐dependent cation current and evokes spiking in bag cell neurons. The present study tests if DAG and IP3 impact the H2O2‐induced current and excitability. In whole‐cell voltage‐clamped cultured bag cell neurons, bath‐application of 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG), a DAG analogue, enhanced the H2O2‐induced current, which was amplified by the inclusion of IP3 in the pipette. A similar outcome was produced by the PLC activator, N‐(3‐trifluoromethylphenyl)‐2,4,6‐trimethylbenzenesulfonamide. In current‐clamp, OAG or OAG plus IP3, elevated the frequency of H2O2‐induced bursting. PKC is also triggered during the afterdischarge; when PKC was stimulated with phorbol 12‐myristate 13‐acetate, it caused a voltage‐dependent inward current with a reversal potential similar to the H2O2‐induced current. Furthermore, PKC activation followed by H2O2 reduced the onset latency and increased the duration of action potential firing. Finally, inhibiting nicotinamide adenine dinucleotide phosphate oxidase with 3‐benzyl‐7‐(2‐benzoxazolyl)thio‐1,2,3‐triazolo[4,5‐d]pyrimidine diminished evoked bursting in isolated bag cell neuron clusters. These results suggest that reactive oxygen species and phosphoinostide metabolites may synergize and contribute to reproductive behaviour by promoting neuroendocrine cell firing. Key points Aplysia bag cell neurons secrete reproductive hormone during a lengthy burst of action potentials, known as the afterdischarge. During the afterdischarge, phospholipase C (PLC) hydrolyses phosphatidylinositol‐4,5‐bisphosphate into diacylglycerol (DAG) and inositol trisphosphate (IP3). Subsequent activation of protein kinase C (PKC) leads to H2O2 production. H2O2 evokes a voltage‐dependent inward current and action potential firing. Both a DAG analogue, 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG), and IP3 enhance the H2O2‐induced current, which is mimicked by the PLC activator, N‐(3‐trifluoromethylphenyl)‐2,4,6‐trimethylbenzenesulfonamide. The frequency of H2O2‐evoked afterdischarge‐like bursting is augmented by OAG or OAG plus IP3. Stimulating PKC with phorbol 12‐myristate 13‐acetate shortens the latency and increases the duration of H2O2‐induced bursts. The nicotinamide adenine dinucleotide phosphate oxidase inhibitor, 3‐benzyl‐7‐(2‐benzoxazolyl)thio‐1,2,3‐triazolo[4,5‐d]pyrimidine, attenuates burst firing in bag cell neuron clusters.

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“…Phosphatidylinositol plays a very important role in cell morphology, metabolic regulation, signaling, and various physiological functions ( Balla, 2013 ). In signaling pathways, phosphatidylinositol 4,5-bisphosphate (PIP2) is hydrolyzed into two messengers: inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG)( Chauhan-Puri et al., 2021 ). Research has demonstrated that the Ca 2+ -dependent signal mediated by the IP3 receptor is the basis of glial cell transmission and is a component of physiological processes such as sleep, neuroprotection, and learning memory ( Chen et al., 2013 ; Nadjar et al., 2013 ).…”