Hydrogen-isotopic variability in fatty acids from Yellowstone National Park hot spring microbial communities

Osburn, M. R.; Sessions, Alex L.; Pepe-Ranney, Charles; Spear, John R.

doi:10.1016/j.gca.2011.05.038

Cited by 58 publications

(61 citation statements)

References 51 publications

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“…Furthermore, the values of S, S R and E 2 : E 3 , are smaller in the vicinity of the river outlet by about 2, 0.5 and 3 times, respectively, than in the open sea, depicting a presence of higher molecular weighted molecules in the estuarine waters, in both the SML and SS. Hence, the higher values of S R indicate an increase in absorption in a shortwavelength range (via an increase in concentration of lowweighted molecules) and a decrease in absorption in a longer wavelength range (a decrease in the concentration of big and more condensed and high-weighted molecules) (Helms et al, 2008;Peravuori and Pihlaja, 1997;Osburn et al, 2011). However, in the vicinity of the river mouth (W1), the studied absorption indices reached lower values in the SML than in the SS.…”

Section: The Absorption and Fluorescence Dependencesmentioning

confidence: 99%

“…The surface microlayer is almost ubiquitous and covers most of the surface of the ocean, even under high turbulence conditions (Cuncliffe et al, 2013). Surface active molecules present in the SML may modify a number of physical processes occurring in the sur-face microlayer: surfactants affect the solar radiation penetration depth (Santos et al, 2012;Carlucci et al, 1985), exchange of momentum between atmosphere and ocean by reducing the sea surface roughness (Nightingale et al, 2000;Frew et al, 1990) and gas exchange between ocean and atmosphere, impacting production of aerosols from the sea surface (Vaishaya et al, 2012;Ostrowska et al, 2015;Petelski et al, 2014). Therefore, research on the influence of surfactants on the sea surface properties becomes an important task, especially in coastal waters and in the vicinity of the river mouths (Maciejewska and Pempkowiak, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Study on organic matter fractions in the surface microlayer in the Baltic Sea by spectrophotometric and spectrofluorometric methods

et al. 2017

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Abstract. The fluorescence and absorption measurements of the samples collected from a surface microlayer (SML) and a subsurface layer (SS), at a depth of 1 m, were studied during three research cruises in the Baltic Sea along with hydrophysical studies and meteorological observations. Several absorption (E 2 : E 3 , S, S R ) and fluorescence (fluorescence intensities at Coble classified peaks: A, C, M, T the ratio (M + T ) / (A + C) , HIX (humification index)) indices of colored and fluorescent dissolved organic matter (CDOM and FDOM) helped to describe the changes in molecular size and weight as well as in composition of organic matter. The investigation allowed the assessment of a decrease in the contribution of two terrestrial components (A and C) with increasing salinity (∼ 1.64 and ∼ 1.89 % in the SML and ∼ 0.78 and ∼ 0.71 % in the SS, respectively) and an increase in components produced in situ (M and T ) with salinity (∼ 0.52 and ∼ 2.83 % in the SML and ∼ 0.98 and ∼ 1.87 % in the SS, respectively). Hence, a component T reveals the biggest relative changes along the transect from the Vistula River outlet to Gdansk Deep, in both the SML and SS, although an increase was higher in the SML than in the SS (∼ 18.5 and ∼ 12.3 %, respectively). The ratio E 2 : E 3 points to greater changes in the molecular weight of CDOM affected by a higher rate of photobleaching in the SML. The HIX index reflects a more advanced stage of humification and condensation processes in the SS. Finally, the results reveal a higher rate of degradation processes occurring in the SML than in the SS. Thus, the specific physical properties of surface active organic molecules (surfactants) may modify, in a specific way, the solar light spectrum entering the sea and a penetration depth of the solar radiation. Research on the influence of surfactants on the physical processes linked to the sea surface becomes an important task, especially in coastal waters and in the vicinity of the river mouths.

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Section: The Absorption and Fluorescence Dependencesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study on organic matter fractions in the surface microlayer in the Baltic Sea by spectrophotometric and spectrofluorometric methods

et al. 2017

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“…Clone library and sample preparation DNA samples from Yellowstone National Park hot springs were collected and prepared as previously reported (Osburn et al, 2011). All other samples were extracted with the UltraClean Soil DNA Isolation Kit (MoBio, Carlsbad, CA, USA).…”

Section: Distribution Of Hpnp In Metagenomesmentioning

confidence: 99%

Diverse capacity for 2-methylhopanoid production correlates with a specific ecological niche

et al. 2013

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Molecular fossils of 2-methylhopanoids are prominent biomarkers in modern and ancient sediments that have been used as proxies for cyanobacteria and their main metabolism, oxygenic photosynthesis. However, substantial culture and genomic-based evidence now indicates that organisms other than cyanobacteria can make 2-methylhopanoids. Because few data directly address which organisms produce 2-methylhopanoids in the environment, we used metagenomic and clone library methods to determine the environmental diversity of hpnP, the gene encoding the C-2 hopanoid methylase. Here we show that hpnP copies from alphaproteobacteria and as yet uncultured organisms are found in diverse modern environments, including some modern habitats representative of those preserved in the rock record. In contrast, cyanobacterial hpnP genes are rarer and tend to be localized to specific habitats. To move beyond understanding the taxonomic distribution of environmental 2-methylhopanoid producers, we asked whether hpnP presence might track with particular variables. We found hpnP to be significantly correlated with organisms, metabolisms and environments known to support plant-microbe interactions (P-valueo10 À 6 ); in addition, we observed diverse hpnP types in closely packed microbial communities from other environments, including stromatolites, hot springs and hypersaline microbial mats. The common features of these niches indicate that 2-methylhopanoids are enriched in sessile microbial communities inhabiting environments low in oxygen and fixed nitrogen with high osmolarity. Our results support the earlier conclusion that 2-methylhopanoids are not reliable biomarkers for cyanobacteria or any other taxonomic group, and raise the new hypothesis that, instead, they are indicators of a specific environmental niche.

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“…Compound-specific hydrogen isotope measurements of lipid biomarkers are an emerging tool with diverse applications to microbial community dynamics (Osburn et al, 2011;Heinzelmann et al, 2016), organic matter cycling (Jones et al, 2008;Li et al, 2009), and paleoclimatology (Sachse et al, 2012, and sources therein). The hydrogen isotopic composition of source water exerts a firstPublished by Copernicus Publications on behalf of the European Geosciences Union.…”

Section: Introductionmentioning

confidence: 99%

“…For short-chain (C < 20) fatty acids, which can be synthesized by a diverse range of organisms, including photoautotrophs, chemoautotrophs, and heterotrophs, core metabolism typically exerts a large control on α lipid-water , with variability in δ 2 H values exceeding 500 ‰ for organisms grown on the same source water (Zhang et al, 2009a;Osburn et al, 2011;Heinzelmann et al, 2015a;Osburn et al, 2016). These metabolic differences have led to the suggestion that δ 2 H values of short-chain fatty acids can be used as an indicator of net community metabolism (Zhang et al, 2009a;Osburn et al, 2011;Heinzelmann et al, 2016;Osburn et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Interplay of community dynamics, temperature, and productivity on the hydrogen isotope signatures of lipid biomarkers

2017

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Abstract. The hydrogen isotopic composition (δ 2 H) of lipid biomarkers has diverse applications in the fields of paleoclimatology, biogeochemistry, and microbial community dynamics. Large changes in hydrogen isotope fractionation have been observed among microbes with differing core metabolisms, while environmental factors including temperature and nutrient availability can affect isotope fractionation by photoautotrophs. Much effort has gone into studying these effects under laboratory conditions with single species cultures. Moving beyond controlled environments and quantifying the natural extent of these changes in freshwater lacustrine settings and identifying their causes is essential for robust application of δ 2 H values of common short-chain fatty acids as a proxy of net community metabolism and of phytoplankton-specific biomarkers as a paleohydrologic proxy.This work targets the effect of community dynamics, temperature, and productivity on 2 H/ 1 H fractionation in lipid biomarkers through a comparative time series in two central Swiss lakes: eutrophic Lake Greifen and oligotrophic Lake Lucerne. Particulate organic matter was collected from surface waters at six time points throughout the spring and summer of 2015, and δ 2 H values of short-chain fatty acids, as well as chlorophyll-derived phytol and the diatom biomarker brassicasterol, were measured. We paired these measurements with in situ incubations conducted with NaH 13 CO 3 , which were used to calculate the production rates of individual lipids in lake surface water. As algal productivity in-

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Hydrogen-isotopic variability in fatty acids from Yellowstone National Park hot spring microbial communities

Cited by 58 publications

References 51 publications

Study on organic matter fractions in the surface microlayer in the Baltic Sea by spectrophotometric and spectrofluorometric methods

Study on organic matter fractions in the surface microlayer in the Baltic Sea by spectrophotometric and spectrofluorometric methods

Diverse capacity for 2-methylhopanoid production correlates with a specific ecological niche

Interplay of community dynamics, temperature, and productivity on the hydrogen isotope signatures of lipid biomarkers

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