Hydrogen ion buffers for biological research

Ferguson, Wilfred J.; Braunschweiger, Karen; Braunschweiger, Walter R.; Smith, James R.; McCormick, J. Justin; Wasmann, Cathy C.; Jarvis, N.; Bell, Duncan H.; Good, Norman E.

doi:10.1016/0003-2697(80)90079-2

Cited by 357 publications

(248 citation statements)

References 10 publications

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“…5 Phosphates also demonstrate complexing capabilities with polyvalent cations and can therefore inhibit a series of metal ion-dependent biochemical reactions. 6 Citrate and succinate form complexes with various cations. 6 Imidazole is used to prepare buffers in the pH range of 6.2-7.8 at 25 C and is also a chelator of various divalent cations.…”

mentioning

confidence: 99%

“…6 Citrate and succinate form complexes with various cations. 6 Imidazole is used to prepare buffers in the pH range of 6.2-7.8 at 25 C and is also a chelator of various divalent cations. 6 Tris is (7) nally, they should be easy to prepare and inexpensive.…”

mentioning

confidence: 99%

“…6 Imidazole is used to prepare buffers in the pH range of 6.2-7.8 at 25 C and is also a chelator of various divalent cations. 6 Tris is (7) nally, they should be easy to prepare and inexpensive. Zwitterionic N-substituted aminosulfonic acids seemed to…”

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(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

et al. 2015

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The use of buffers to maintain the pH within a desired range is a very common practice in chemical, biochemical and biological studies.Among them, zwitterionic N-substituted aminosulfonic acids, usually known as Good'sbuffers, although widely used, can complex metals and interact with biological systems. The present work reviews, discusses and updates the metal complexation characteristics of thirty one commercially available buffers. In addition, their impact on biological systems is also presented. The influences of these buffers on the results obtained in biological, biochemical and environmental studies, with special focus on their interaction with metal ions, are highlighted and critically reviewed. Using chemical speciation simulations, based on the current knowledge of the metal-buffer stability constants, a proposal of the most adequate buffer to employ for a given metal ion is presented.

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(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

et al. 2015

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“…The requirements of the buffer are simply that it has an ionizing functional group with a pK in the pH range required. Ideally the buffer should be inert as far as the biological system is concerned and fulfil certain other criteria also (Good et al, 1966;Ferguson et al, 1980). Relatively few buffers have been available for the pH range 6-8 and, of those tested, HEPES, introduced by Good et al (1966), has become one of the most successful and widely used (Ferguson et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…Ideally the buffer should be inert as far as the biological system is concerned and fulfil certain other criteria also (Good et al, 1966;Ferguson et al, 1980). Relatively few buffers have been available for the pH range 6-8 and, of those tested, HEPES, introduced by Good et al (1966), has become one of the most successful and widely used (Ferguson et al, 1980). A perusal of the recent cyanobacterial literature reveals that HEPES is overwhelmingly the most widely used buffer, both for experiments with intact cells and those with cell-free extracts.…”

Section: Introductionmentioning

confidence: 99%

Buffer and Salt Damage to the Filamentous Cyanobacterium Anabaena cylindrica

1983

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The commonly used buffer HEPES was found to cause severe disintegration of the cyanobacterium Anabaena cylindrica, as measured by filament breakage, cell disruption, phycocyanin release and nitrogenase inhibition. The effect became increasingly severe as the buffer concentration was increased above 10 mM. The observed cell damage does not appear to be unique to HEPES, similar observations being made with Tris/HCl, sodium phosphate, sodium sulphate and sodium chloride. It appears that the cells are very sensitive to ionic strength.

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Influence ofN-Substituted Aminosulfonic Acids with a Morpholinic Ring pH Buffers on the Redox Processes of Copper or Zinc Ions: A Contribution to Speciation Studies

1999

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The electrochemical behavior of copper(II) or zinc(II) metal ions was investigated in the presence of different pH buffers [3‐(N‐morpholino)‐2‐hydroxypropanesulfonic acid (MOPSO), 2‐(N‐morpholino)ethanesulfonic acid (MES) and 3‐(N‐morpholino)propanesulfonic acid (MOPS)] by differential pulse anodic stripping voltammetry (DPASV) and differential pulse polarography (DPP). These studies showed that these pH buffers did not complex zinc or copper, but MOPSO and MOPS modified the reversibility of copper electrochemical DPASV and DPP (only MOPSO) responses, under the experimental conditions used. Depression of peak current for both metal ions was also observed in the presence of all pH buffers due to surface coverage of the electrode by those compounds, which was verified by alternating current voltammetry (ACV).

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Hydrogen ion buffers for biological research

Cited by 357 publications

References 10 publications

(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review

Buffer and Salt Damage to the Filamentous Cyanobacterium Anabaena cylindrica

Influence ofN-Substituted Aminosulfonic Acids with a Morpholinic Ring pH Buffers on the Redox Processes of Copper or Zinc Ions: A Contribution to Speciation Studies

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