Hydrogen inhalation alleviates nonalcoholic fatty liver disease in metabolic syndrome rats

Liu, Boyan; Xue, Junli; Zhang, Mengyu; Wang, Mingyue; Ma, Tingting; Zhao, Min; Gu, Qianqian; Qin, Shucun

doi:10.3892/mmr.2020.11364

Cited by 14 publications

(18 citation statements)

References 60 publications

(77 reference statements)

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“…In addition to the inhibition of JNK activation, H 2 has been shown to attenuate the activation of NF-κB in an LPS-induced cardiac dysfunction mice model ( 20 ). H 2 has also been shown to increase the hepatic expression of nuclear factor erythroid 2-related factor 2 (Nrf2) ( 42 ) and PPAR-α ( 43 ) and reduced the hepatic expression of SREBP-1c ( 44 ) in NAFLD animal models. These transcription factors are essential mediators of inflammation (NF-κB), oxidative stress (Nrf2) and lipid metabolism (SREBP-1c and PPAR-α), where they have been reported to serve key roles in the pathogenesis of both ALD and NAFLD ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

Zhang

Chen

et al. 2021

Exp Ther Med

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Binge alcohol drinking is fast becoming a global health concern, with the liver among the first organ involved and the one afflicted with the greatest degree of injury. Oxidative stress, alterations in hepatic metabolism, immunity and inflammation have all been reported to contribute to the development of alcoholic liver disease (ALD). Hydrogen gas (H 2) serves a key role in the modulation of hepatic redox, immune and inflammatory homeostasis. However, the effects of treatment using intraperitoneal injection of H 2 on ALD remain unexplored. Therefore, the aim of the present study was to investigate the effects and underlying mechanism of intraperitoneal injection of H 2 on acute alcohol-induced liver injury in a mouse model. H 2 was administered by daily intraperitoneal injections (1.0 ml/100 g) for 4 days. On day 4, the mice received H 2 after fasting for 5.5 h. After 30 min, the mice were administered with 33% (v/v) ethanol at a cumulative dose of 4.5 g/kg body weight by four equally divided gavages at 20-min intervals. Blood and liver tissues were collected at 16 h after the first ethanol gavage. Subsequently, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride and total cholesterol (TC) levels were analyzed using an Automatic Clinical Analyzer. Hepatic JNK activity and GAPDH levels were examined by western blotting. It was observed that acute ethanol gavage induced liver injury, as indicated by significantly increased serum ALT and AST levels, which were effectively decreased by H 2 at 16 h after the first ethanol gavage. In addition, H 2 treatment reduced serum TC levels in the Alcohol+H 2 group when compared with those in Alcohol group. Mechanistically, H 2 attenuated hepatic JNK phosphorylation induced by acute ethanol gavage. Therefore, the results of the present study demonstrated that treatment with exogenous H 2 by intraperitoneal injection may alleviate acute alcohol-induced liver injury by inhibiting hepatic JNK activation, which may represent a novel therapeutic strategy for ALD.

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Section: Discussionmentioning

confidence: 99%

Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

Zhang

Chen

et al. 2021

Exp Ther Med

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“…A large number of studies have since shown the therapeutic and preventive effects of H 2 in various animal disease models. 2 3 4 5 Meanwhile, some clinical investigations have also confirmed its beneficial effects on different diseases. 6 7 Various ways have been explored to administer H 2 , mainly including inhaling H 2 , drinking H 2 -rich water, injecting H 2 -rich saline, and direct incorporation of H 2 by diffusion (such as bath and eye-drops).…”

Section: Introductionmentioning

confidence: 97%

In vivo microelectrode monitoring of real-time hydrogen concentration in different tissues of rats after inhaling hydrogen gas

Liu

Xue

et al. 2022

Med Gas Res

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Medical effects of hydrogen have been reported in many studies. Due to difficulties in measuring hydrogen concentration in vivo after intake and high explosive risks of hydrogen, studies about dose-response relationships and tissue concentrations of hydrogen are few. Here, for the first time, we monitored real-time hydrogen concentrations in different tissues in rats including brain, liver, spleen, kidney, thigh muscle, inguinal white adipose tissue, and gonadal white adipose tissue after inhaling different concentrations of hydrogen (4%, 42%, and 67%) using an electrochemical sensor. Hydrogen concentrations in the same tissue showed a dose-dependent response. The equilibrium concentration values were highest in the brain and lowest in the thigh muscle. The saturation and desaturation curves changed more slowly in the thigh muscle and white adipose tissues than in other tissues. These results provide fundamental information for the selection of hydrogen dose applications in basic research and clinical trials. The experiments were approved by the Laboratory Animal Ethics Committee of Shandong First Medical University & Shandong Academy of Medical Sciences (No. 2020-1028) on March 18, 2020.

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“…4 Since then, several researchers have confirmed the therapeutic and preventive effects of H 2 in various clinical trials and animal disease models. [5][6][7] H 2 therapy has been applied in aging-related diseases, such as type 2 diabetes, atherosclerosis, Parkinson's disease, and Alzheimer's disease. [6][7][8] H 2 can be administered via different routes such as H 2 gas inhalation, 5 H 2 -rich water (HRW) drinking, 9 H 2 -rich saline (HRS) injection, 10 and direct incorporation of H 2 through diffusion (such as taking H 2water bath 11 or dropping H 2 -saline into the eyes).…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7] H 2 therapy has been applied in aging-related diseases, such as type 2 diabetes, atherosclerosis, Parkinson's disease, and Alzheimer's disease. [6][7][8] H 2 can be administered via different routes such as H 2 gas inhalation, 5 H 2 -rich water (HRW) drinking, 9 H 2 -rich saline (HRS) injection, 10 and direct incorporation of H 2 through diffusion (such as taking H 2water bath 11 or dropping H 2 -saline into the eyes). 12 Previous studies have mainly focused on the effectiveness of H 2 administered via a specific route.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Molecular Hydrogen Following Different Routes of Administration on D-Galactose-Induced Aging Mice

Liu¹,

Xie²,

Xue³

et al. 2021

JIR

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Background: Molecular hydrogen (H 2 ) has been recognized as an effective antioxidant with no or little side effects. While it is known that oxidative stress is closely associated with aging, the beneficial effect of H 2 on oxidative stress-related aging is still unclear. In this study, a mouse model of D-galactose-induced aging was employed to investigate the protective effects of H 2 . Methods: The mice were administrated of H 2 via different routes (4% H 2 inhalation, H 2rich water drinking, and H 2 -rich saline injection), the aging-related biomarkers in plasma and the oxidative stress in different tissues were measured. Results:The results showed that H 2 improved aging-related biomarkers, ie, total antioxidant capacity, advanced glycation end products, tumor necrosis factor-α, free fatty acids, and alanine aminotransferase in plasma. Furthermore, H 2 alleviated oxidative stress in the liver, brain, and heart by reducing the levels of lipid peroxidation and malondialdehyde and increasing the activity of superoxide dismutase. In addition, it seems that 4% H 2 inhalation was the most effective regarding the amount of H 2 taken up and in reducing the markers of oxidative stress in some of the tissues; however, the other routes of administration resulted in the same efficacy in most indicators. Conclusion: H 2 can prevent oxidative stress in D-galactose-induced aging mice when administered by different routes.

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Hydrogen inhalation alleviates nonalcoholic fatty liver disease in metabolic syndrome rats

Cited by 14 publications

References 60 publications

Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

Hydrogen gas alleviates acute alcohol‑induced liver injury by inhibiting JNK activation

In vivo microelectrode monitoring of real-time hydrogen concentration in different tissues of rats after inhaling hydrogen gas

Protective Effect of Molecular Hydrogen Following Different Routes of Administration on D-Galactose-Induced Aging Mice

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