Hydrogen Gas Enhanced Seed Germination by Increasing Trehalose Biosynthesis in Cucumber

Huang, Panpan; Li, Changxia; Che, Pengpeng; Liu, Huwei; Zhao, Zongxi; Feng, Li; Liu, Xinjuan; Liao, Weibiao

doi:10.1007/s00344-022-10858-0

Cited by 2 publications

(3 citation statements)

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“…HRW treatment promoted the occurrence of cucumber adventitious roots by increasing glucose, sucrose, and water-soluble carbohydrate content [41]. Huang et al [38] found that HRW could accelerate starch degradation and increase sugar accumulation to promoted seed germination in cucumber. Sugar always plays an energy source role in plant growth and development.…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme activity of α-amylase, β-amylase, total amylase, sucrose synthase, and sucrose phosphate synthase were determined by the ELISA method (ELISA kit: Yuanmu Biotechnology Co., Ltd., Shanghai, China) [38]. The enzyme solutions were extracted according to the instructions as follows: fresh sample (0.5 g) was quickly ground in liquid nitrogen and transferred to the test tube.…”

Section: Measurement Of Enzyme Activitiesmentioning

confidence: 99%

“…A previous study has shown that α-amylase and β-amylase degrade starch into dextrin, which is then converted into maltose and glucose [37]. During cucumber seed germination, HRW improved the content of glucose and sucrose by activating the activity of α-amylase, β-amylase, and total amylase, thereby promoting seed germination [38]. Therefore, plants may accumulate a large amount of water-soluble carbohydrate, glucose, and fructose nutrients with the degradation of starch.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

et al. 2023

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Hydrogen gas (H2) is considered as a signaling molecule and plays multiple roles in plant growth. However, the effect of H2 on postharvest physiology in lily scales during storage has not been reported. In this study, the regulatory roles of hydrogen-rich water (HRW, a H2 donor, a concentration of 0.45 mM for 100% HRW) in water status, ion balance, and nutrients in Lanzhou lily (Lilium davidii var. unicolor) scales were investigated. The scales were soaked in HRW for 12 d, and sampling was performed every 3 d for a total of 5 times. The results show that HRW (0, 10, 50, and 100%) increased the fresh weight, dry weight, relative water content, and water loss rate in lily scales, with maximum biological response at 50% HRW. Treatment with 50% HRW significantly increased the K+ content and K+/Na+ ratio in lily scales and decreased Na+ content. The Na+ K+-ATPase, and PM H+-ATPase activities were also increased by 50% HRW treatment. Meanwhile, 50% HRW up-regulated the expression of AKT1 and HA3 genes and down-regulated the expression of NHX2 and SOS1 genes. In addition, 50% HRW treatment significantly increased the expression level of PIP1;5, PIP2A, TIP1;3, and TIP2;2 genes. Treatment with 50% HRW significantly increased the content of water-soluble carbohydrate, sucrose, glucose, and fructose in lily scales, and decreased the content of starch. In addition, 50% HRW treatment significantly increased the activity of α-amylase, β-amylase, total amylase, sucrose synthase, and sucrose phosphate synthase. Collectively, H2 might enhance the water retention capacity and nutrient content in lily scales by maintaining ion balance, regulating aquaporin, and increasing sugar-metabolizing enzyme activity, thereby prolonging the storage period of postharvest scales of Lanzhou lily.

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Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Enzyme Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

et al. 2023

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Physiological and Biochemical Changes in the Seeds of Naturally Aged Wenling Medic (Medicago polymorpha) with Its Recovery of Viability

Wei

Min

et al. 2023

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Wenling Medic (Medicago polymorpha) is common in southern China and has long been utilized as a vegetable in eastern China, as well as a significant raw ingredient for livestock and pickled meals. As a legume, there is still a research vacuum, and the first problem Wenling Medic faces in production is the problem of seed germination. The germination percentage of Wenling Medic was low, according to production practice and laboratory tests performed in this work. Furthermore, after more than two years of storage, the germination percentage of Wenling Medic dropped sharply, and it lacked a long enough seed life. An attempt was made to restore the viability of the seeds using the polyethylene glycol (PEG) osmoregulation-mediated priming method, after the physiological and biochemical characteristics of the seeds were assessed using the anthrone method, Bradford assay, 3,5-dinitrosalicylic acid (DNS) method, and thiobarbituric acid (TBA) method. The findings showed that it has a relatively short storage age and, with a useable life of only two years under normal aging circumstances, is not deactivated soon after harvesting. In addition, whereas protein content and α-amylase concentration did not significantly correlate (p > 0.05) with storage life, the conductivity of exudate, malondialdehyde content, and carbohydrate content did (p < 0.05). The seed viability was not considerably increased by the saturation initiation mediated by osmoregulation, utilizing PEG. In conclusion, the decrease in Wenling Medic seeds’ germination ability was substantially connected with higher levels of lipid peroxidation and decreased carbohydrate levels, but not with protein concentrations or α-amylase activity. The timing of dehydration may need to be carefully controlled when using PEG osmoregulation to prime Wenling Medic seeds.

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Hydrogen Gas Enhanced Seed Germination by Increasing Trehalose Biosynthesis in Cucumber

Cited by 2 publications

References 56 publications

Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage

Physiological and Biochemical Changes in the Seeds of Naturally Aged Wenling Medic (Medicago polymorpha) with Its Recovery of Viability

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