Hydrogen formation by an arsenate-reducing Pseudomonas putida, isolated from arsenic-contaminated groundwater in West Bengal, India

Freikowski, Dominik; Winter, Josef; Gallert, Claudia

doi:10.1007/s00253-010-2856-0

Cited by 37 publications

(13 citation statements)

References 26 publications

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“…The average abundances of Alishewanella, Psychrobacter , Pseudomonadaceae, Methylotenera , Comamonadaceae, and Crenothrix were elevated in high As groundwater samples than in low As samples, while Rheinheimera and unidentified OP3 presented higher abundance in low As groundwater samples than high As samples ( Figure 3B ; Table 4 ). Although Acinetobacter and Pseudomonas had been reported to be correlated with As metabolism (Anderson and Cook, 2004; Chang et al, 2009; Freikowski et al, 2010), these two populations appeared with slightly higher percentages in low As groundwater samples but dominated in both high (0.2–28.2% and 0.1–13.6%, respectively) and low (0.2–41.4% and 0.2–37.3%, respectively) As samples in the present study. This might be due to the wide occurrence of these two kinds of bacteria in nature, and some of their isolates are capable of tolerating high concentrations of As.…”

Section: Resultscontrasting

confidence: 60%

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Wang

Jiang

et al. 2016

Front. Microbiol.

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Microbial communities can play important role in arsenic release in groundwater aquifers. To investigate the microbial communities in high arsenic groundwater aquifers in agricultural irrigation area, 17 groundwater samples with different arsenic concentrations were collected along the agricultural drainage channels of Hangjinhouqi County, Inner Mongolia and examined by illumina MiSeq sequencing approach targeting the V4 region of the 16S rRNA genes. Both principal component analysis and hierarchical clustering results indicated that these samples were divided into two groups (high and low arsenic groups) according to the variation of geochemical characteristics. Arsenic concentrations showed strongly positive correlations with NH4+ and total organic carbon (TOC). Sequencing results revealed that a total of 329–2823 operational taxonomic units (OTUs) were observed at the 97% OTU level. Microbial richness and diversity of high arsenic groundwater samples along the drainage channels were lower than those of low arsenic groundwater samples but higher than those of high arsenic groundwaters from strongly reducing areas. The microbial community structure in groundwater along the drainage channels was different from those in strongly reducing arsenic-rich aquifers of Hetao Plain and other high arsenic groundwater aquifers including Bangladesh, West Bengal, and Vietnam. Acinetobacter and Pseudomonas dominated with high percentages in both high and low arsenic groundwaters. Alishewanella, Psychrobacter, Methylotenera, and Crenothrix showed relatively high abundances in high arsenic groundwater, while Rheinheimera and the unidentified OP3 were predominant populations in low arsenic groundwater. Archaeal populations displayed a low occurrence and mainly dominated by methanogens such as Methanocorpusculum and Methanospirillum. Microbial community compositions were different between high and low arsenic groundwater samples based on the results of principal coordinate analysis and co-inertia analysis. Other geochemical variables including TOC, NH4+, oxidation-reduction potential, and Fe might also affect the microbial composition.

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Section: Resultscontrasting

confidence: 60%

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Wang

Jiang

et al. 2016

Front. Microbiol.

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“…At least 8 bacterial species closely related to those known to metabolize arsenic were identified at an abundance below 1% across various depths in the aquifer sediments at site F and included respiratory arsenate reducers (carrying the arr gene) implicated in mobilizing arsenic ( 30 ), detoxifying arsenate reducers (carrying ars genes), and close relatives to the aerobic arsenite oxidizers carrying the aio genes. For example, a close relative to Pseudomonas putida strain WB (100% identity), a dissimilatory arsenic-respiring bacterium (carrying both arrA and arsC genes [ 64 ]), was found at depths of 7.7, 16.9, and 26 m comprising less than 0.1% of the population. An organism most closely related to Geobacter lovleyi (93% identity), a dissimilatory Fe(III)-reducing bacterium that is also implicated in respiring As(V) ( 65 ), was found at depths of 7.7, 10.8, and 16.9 m at a relative abundance of below 0.1%.…”

Section: Resultsmentioning

confidence: 99%

Microbial Community Structure and Arsenic Biogeochemistry in Two Arsenic-Impacted Aquifers in Bangladesh

Gnanaprakasam

Lloyd

Boothman

et al. 2017

mBio

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Long-term exposure to trace levels of arsenic (As) in shallow groundwater used for drinking and irrigation puts millions of people at risk of chronic disease. Although microbial processes are implicated in mobilizing arsenic from aquifer sediments into groundwater, the precise mechanism remains ambiguous. The goal of this work was to target, for the first time, a comprehensive suite of state-of-the-art molecular techniques in order to better constrain the relationship between indigenous microbial communities and the iron and arsenic mineral phases present in sediments at two well-characterized arsenic-impacted aquifers in Bangladesh. At both sites, arsenate [As(V)] was the major species of As present in sediments at depths with low aqueous As concentrations, while most sediment As was arsenite [As(III)] at depths with elevated aqueous As concentrations. This is consistent with a role for the microbial As(V) reduction in mobilizing arsenic. 16S rRNA gene analysis indicates that the arsenic-rich sediments were colonized by diverse bacterial communities implicated in both dissimilatory Fe(III) and As(V) reduction, while the correlation analyses involved phylogenetic groups not normally associated with As mobilization. Findings suggest that direct As redox transformations are central to arsenic fate and transport and that there is a residual reactive pool of both As(V) and Fe(III) in deeper sediments that could be released by microbial respiration in response to hydrologic perturbation, such as increased groundwater pumping that introduces reactive organic carbon to depth.

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“…strain GW7, Alishewanella sp. strain GIDC-5, Pseudomonas mendocina strain GW9 and Pseudomonas putida strain WB have been found to be involved in As cycling including arsenic resistance, arsenic reduction and oxidation [ 25 , 52 , 60 ]. In addition to Pseudomonas and Arthrobacter , some oxidizing bacteria such as Thiobacillus , Hydrogenophaga , and Sulfuricurvum also dominated our high arsenic sediments.…”

Section: Discussionmentioning

confidence: 99%

Microbial Community in High Arsenic Shallow Groundwater Aquifers in Hetao Basin of Inner Mongolia, China

Wang

Dai

et al. 2015

PLoS ONE

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A survey was carried out on the microbial community of 20 groundwater samples (4 low and 16 high arsenic groundwater) and 19 sediments from three boreholes (two high arsenic and one low arsenic boreholes) in a high arsenic groundwater system located in Hetao Basin, Inner Mongolia, using the 454 pyrosequencing approach. A total of 233,704 sequence reads were obtained and classified into 12–267 operational taxonomic units (OTUs). Groundwater and sediment samples were divided into low and high arsenic groups based on measured geochemical parameters and microbial communities, by hierarchical clustering and principal coordinates analysis. Richness and diversity of the microbial communities in high arsenic sediments are higher than those in high arsenic groundwater. Microbial community structure was significantly different either between low and high arsenic samples or between groundwater and sediments. Acinetobacter, Pseudomonas, Psychrobacter and Alishewanella were the top four genera in high arsenic groundwater, while Thiobacillus, Pseudomonas, Hydrogenophaga, Enterobacteriaceae, Sulfuricurvum and Arthrobacter dominated high arsenic sediments. Archaeal sequences in high arsenic groundwater were mostly related to methanogens. Biota-environment matching and co-inertia analyses showed that arsenic, total organic carbon, SO4 2-, SO4 2-/total sulfur ratio, and Fe2+ were important environmental factors shaping the observed microbial communities. The results of this study expand our current understanding of microbial ecology in high arsenic groundwater aquifers and emphasize the potential importance of microbes in arsenic transformation in the Hetao Basin, Inner Mongolia.

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Hydrogen formation by an arsenate-reducing Pseudomonas putida, isolated from arsenic-contaminated groundwater in West Bengal, India

Cited by 37 publications

References 26 publications

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Microbial Community Structure and Arsenic Biogeochemistry in Two Arsenic-Impacted Aquifers in Bangladesh

Microbial Community in High Arsenic Shallow Groundwater Aquifers in Hetao Basin of Inner Mongolia, China

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