Hydrogen cycling as a general mechanism for energy coupling in the sulfate-reducing bacteria, Desulfovibrio sp.

Odom, J. D.

doi:10.1016/0378-1097(81)90035-5

Cited by 94 publications

(138 citation statements)

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“…The discussion however is troubled by the fact that the function of the periplasmic Fe-hydrogenase is still controversial. Odom and Peck suggested a role for the Fe-hydrogenase in the obligate hydrogen-cycling model [28]. In this model the periplasmic Fe-hydrogenase functions as an uptake hydrogenase and delivers its electrons to cytochrome cs, The electrons are subsequently transferred back across the membrane and used in the cytoplasm for the reduction of sulfate.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)

Verhagen

Wolbert

Hagen

1994

European Journal of Biochemistry

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An electrochemical study of the periplasmic cytochrome c553 of Desulfovibrio vulgaris (Hildenborough) is presented. The dependence of the midpoint potential on temperature and pH was studied with cyclic voltammetry. The voltammograms obtained were reversible and revealed that this cytochrome showed fast electron transfer on a bare glassy carbon electrode. The midpoint potential at pH 7.0 and 25°C was found to be 62 mV versus the normal hydrogen electrode. It was observed that the temperature dependence was discontinuous with a transition temperature at 32°C. The standard reaction entropy at the growth temperature of the organism (37°C) was calculated to be AS"'= -234 J mol-' K-I. The pH dependence of the midpoint potential could be described with one pK of the oxidized form with a value of 10.6. The second-order rate constant for electron transfer between cytochrome css3 and the Fe-hydrogenase from D. vulgaris (H) was also determined with cyclic voltammetry. The equivalent rate constant for cytochrome c3 and hydrogenase was measured for comparison. The second-order rate constants are 2X 1O'M-I s-' for cytochrome c5s3 and 2X 10' M-' s-' for cytochrome c3. The kinetic parameters of the hydrogenase for both cytochromes were determined using the spectrophotometric hydrogen consumption assay. With cytochrome css3 this resulted in a K, of 46 pM and a maximum turnover number of 7

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Section: Discussionmentioning

confidence: 99%

Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)

Verhagen

Wolbert

Hagen

1994

European Journal of Biochemistry

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“…A chemiosmotic model for energy transduction from lactate oxidation termed "hydrogen cycling" has been proposed 22 : the protons and electrons produced in lactate and pyruvate oxidation react with a cytoplasmic hydrogenase to form hydrogen, which diffuses across the membrane where it is reoxidized by periplasmic hydrogenases to form a proton gradient (Fig. 2 24 and cooMKLXUHF 13,25 ), which both have active sites facing the cytoplasm.…”

Section: Energy Metabolismmentioning

confidence: 99%

The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough

Heidelberg¹,

Seshadri²,

et al. 2004

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Sulfate-reducing bacteria (SRB) are anaerobic prokaryotes found ubiquitously in nature. SRB were the first nonphotosynthetic, anaerobic bacteria shown to generate energy (ATP) through electron transfer-coupled phosphorylation. For this process, the SRB typically use sulfate as the terminal electron acceptor for respiration of hydrogen or various organic acids, which results in the production of sulfide, a highly reactive and toxic end-product. Beyond their obvious function in the sulfur cycle, SRB play an important role in global cycling of numerous other elements 1 . For example, in the carbon cycle, the SRB form part of microbial consortia that completely mineralize organic carbon in anaerobic environments; polymeric materials (e.g., cellulose) are first depolymerized and metabolized by fermentative microorganisms, and the resulting organic acid and reduced gas (that is, CO and H 2 ) end-products are further fermented or oxidized by other microbes, including SRB. The latter are particularly active in sulfate-rich (e.g., marine) environments, where they effectively link the global sulfur and carbon cycles 1,2 .Beyond these ecological roles, SRB also have a major economic impact because of their involvement in biocorrosion of ferrous metals in anaerobic environments 3 , described as "industrial venereal disease-it's expensive, everybody has it, and nobody wants to talk about it" 4 . For example, because SRB are abundant in oil fields, their metabolism has many negative consequences for the petroleum industry (e.g., corrosion of drilling and pumping machinery and storage tanks, souring of oil by sulfide production, plugging of machinery and rock pores with biomass and sulfide precipitates). The SRB also contribute to bioremediation of toxic metal ions 5,6 . Their metabolism increases the pH, causing toxic metal ions like copper (II), nickel (II) and cadmium (II) to precipitate as metal sulfides in acidic aquatic environments (e.g., mine effluents). Additionally, SRB can deliver electrons directly to oxidized toxic metal ions, including uranium (VI), technetium (VII), and chromium (VI), converting these into less soluble, reduced forms. Hence, SRB-mediated reduction represents a potentially useful mechanism for the bioremediation of metal ion contaminants from anaerobic sediments 6 .Most research on the metabolism and biochemistry of SRB has been done on the genus Desulfovibrio, a member of the δ-proteobacteria The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough Desulfovibrio vulgaris Hildenborough is a model organism for studying the energy metabolism of sulfate-reducing bacteria (SRB) and for understanding the economic impacts of SRB, including biocorrosion of metal infrastructure and bioremediation of toxic metal ions. The 3,570,858 base pair (bp) genome sequence reveals a network of novel c-type cytochromes, connecting multiple periplasmic hydrogenases and formate dehydrogenases, as a key feature of its energy metabolism. The relative arrangement of genes encod...

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“…Conversely, when H 2 is used as the electron donor, a proton gradient can be established directly by periplasmic oxidation of H 2 , although some metabolite cycling is still predicted. production of energy for growth by SRMs, proposed by Odom and Peck 33 and modified by Voordouw 34 : the hydrogen-cycling model (BOX 2). This model posits that hydrogen equivalents that are generated by the oxidation of organic compounds are converted to H 2 by cytoplasmic hydrogenase complexes.…”

mentioning

confidence: 99%

“…Early during its growth on lactate and sulphate (SO 2-), Desulfovibrio vulgaris Hildenborough produces a burst of metabolites such as H , formate and CO. This observation led to the proposal of the hydrogen-cycling model, which tries to explain the growth of this microorganism despite the energetic constraints that are associated with sulphate reduction 33 (see the figure). According to this model, hydrogen equivalents that are generated by the oxidation of organic compounds (such as lactate) are hypothesized to be cycled to the periplasm via the activities of the cytoplasmic hydrogenases Escherichia coli hydrogenase 3 (Ech) and CO-dependent hydrogenase (Coo) (the green pathway in the figure) 34 .…”

mentioning

confidence: 99%

How sulphate-reducing microorganisms cope with stress: lessons from systems biology

et al. 2011

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| Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

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Hydrogen cycling as a general mechanism for energy coupling in the sulfate-reducing bacteria, Desulfovibrio sp.

Cited by 94 publications

References 7 publications

Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)

Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)

The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough

How sulphate-reducing microorganisms cope with stress: lessons from systems biology

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Hydrogen cycling as a general mechanism for energy coupling in the sulfate-reducing bacteria, Desulfovibrio sp.

Cited by 94 publications

References 7 publications

Cytochrome c553 from Desulfovibrio vulgaris (Hildenborough)

Cytochrome c553 from Desulfovibrio vulgaris (Hildenborough)

The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough

How sulphate-reducing microorganisms cope with stress: lessons from systems biology

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Product

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Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)

Cytochrome c₅₅₃ from Desulfovibrio vulgaris (Hildenborough)