Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids

Kalkus, Jutta; Reh, Michael; Schlegel, H. G.

doi:10.1099/00221287-136-6-1145

Cited by 69 publications

(53 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The R. opacus wild-type strains MR11 and MR22 were each shown to contain three linear plasmids, pHG201 (270 kb), pHG202 (400 kb) and pHG203 (420 kb), as well as pHG204 (190 kb), pHG205 (280 kb) and pHG206 (500 kb), respectively (Kalkus et al, 1990). Two of these plasmids, pHG201 and pHG205, as well as pHG207, obtained from the transconjugant strain MR2253 (Kalkus et al, 1993), enable their hosts to grow chemolithoautotrophically on gaseous hydrogen and carbon dioxide.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmids of rhodococci, which, like their Streptomyces counterparts often possess linear topology, frequently encode catabolic genes, enabling their hosts either to grow with unusual carbon and energy sources such as CO 2 / hydrogen (Kalkus et al, 1990), (chloro)biphenyl (Kosono et al, 1997;Masai et al, 1997), and isopropylbenzene (Dabrock et al, 1994;Stecker et al, 2003) or to just convert such compounds as was shown for trichloroethene (Dabrock et al, 1994;Saeki et al, 1999). In addition, linear plasmids of rhodococci were shown to harbour genes for resistance to thallium (Kalkus et al, 1990) and arsenic (Dabrock et al, 1994;Stecker et al, 2003), as well as genes for phytopathogenicity (Maes et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, linear plasmids of rhodococci were shown to harbour genes for resistance to thallium (Kalkus et al, 1990) and arsenic (Dabrock et al, 1994;Stecker et al, 2003), as well as genes for phytopathogenicity (Maes et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

et al. 2004

View full text Add to dashboard Cite

The Gram-positive actinobacterium Rhodococcus opacus 1CP is able to utilize several (chloro)aromatic compounds as sole carbon sources, and gene clusters for various catabolic enzymes and pathways have previously been identified. Pulsed-field gel electrophoresis indicates the occurrence of a 740 kb megaplasmid, designated p1CP. Linear topology and the presence of covalently bound proteins were shown by the unchanged electrophoretic mobility after S1 nuclease treatment and by the immobility of the native plasmid during non-denaturing agarose gel electrophoresis, respectively. Sequence comparisons of both termini revealed a perfect 13 bp terminal inverted repeat (TIR) as part of an imperfect 583/587 bp TIR, as well as two copies of the highly conserved centre (GCTXCGC) of a palindromic motif. An initial restriction analysis of p1CP was performed. By means of PCR and hybridization techniques, p1CP was screened for several genes encoding enzymes of (chloro)aromatic degradation. A single maleylacetate reductase gene macA, the clc gene cluster for 4-chloro-/3,5-dichlorocatechol degradation, and the clc2 gene cluster for 3-chlorocatechol degradation were found on p1CP whereas the cat and pca gene clusters for the catechol and the protocatechuate pathways, respectively, were not. Prolonged cultivation of the wild-type strain 1CP under non-selective conditions led to the isolation of the clc-and clc2-deficient mutants 1CP.01 and 1CP.02 harbouring the shortened plasmid variants p1CP.01 (500 kb) and p1CP.02 (400 kb).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

et al. 2004

View full text Add to dashboard Cite

show abstract

“…The linear form is shared with various transposons (Grindley & Reed, 1985), adenoviruses (Tamanoi & Stillman, 1983) and the bacteriophage 429 from Bacillus subtilis (Escarmis & Salas,198 1). Among eubacteria, Streptomyces (Hayakawa et al, 1979;Hirochika & Sakaguchi, 1982;Hirochika et al, 1984;Kinashi et al, 1987;Kinashi & Shimaji-Murayama, 1991), Nocardia (Kalkus et al, 1990) and Borrelia (Barbour & Garon, 1987) seem to be the only genera having strains that harbour linear plasmids, whereas the great majority of plasmids described in other genera are found as covalently closed circular DNA molecules. Many Bacillus strains carry endogenous plasmids, several of which do not express useful or identifiable genetic markers (Lovett & Bramucci, 1975;Tanaka & Koshikawa, 1977;Tanaka & Sakaguchi, 1978;Uozumi et al, 1980;Gryczan, 1982).…”

Section: Introductionmentioning

confidence: 99%

Isolation and partial characterization of a new linear DNA plasmid isolated from Bacillus polymyxa SCE2

Rosado

Seldin

1993

Journal of General Microbiology

View full text Add to dashboard Cite

A plasmid, designated pFS1, has been identified for the first time in a strain of Bacillus polymyxa. The plasmid is 16 kb in length. It is sensitive to digestion by DNAase I, restriction endonucleases and exonuclease 111, but resistant to RNAase A and I-exonuclease. A restriction map of the plasmid has been constructed, demonstrating its linear nature. The linear DNA molecules of pFSl are attached at their 5' ends to a protein which can be cleaved by Pronase. No homology was detected between pFSl and chromosomal DNAs from a number of different B. pok'ymyxa strains. Also, hybridization between pFSl and total DNA from B. pok'ymyxa SCE2 plasmid-cured derivatives does not occur. The plasmid seems to be cryptic since B. polymyxa strains harbouring pFSl and the cured derivatives have the same phenotype.

show abstract

“…PFGE analysis using different pulse times showed that the relative migration of pSLV195, pSLV45 and pSLV325 remained constant relative to the linear lambda concatemers (data not shown). This indicates that the plasmids are linear rather than circular, since migration rates of circular plasmids change depending on the switch time used during electrophoresis (Kalkus et al, 1990;Kinashi & Shimaji-Murayama, 1991). …”

mentioning

confidence: 99%

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Hosted¹,

Wang²,

Horan³

2004

Microbiology

View full text Add to dashboard Cite

Streptomyces lavendulae IMRU 3455 contains two large linear plasmids designated pSLV45 (45 kb) and pSLV195 (195 kb). A cosmid, pSPRX604, containing 42 kb from pSLV45 was cloned and sequenced. pSLV45 was tagged with a hygromycin-resistance marker by homologous recombination to generate the derivatives pSLV45.680 and pSLV45.681. An apramycin-resistance marker was introduced into S. lavendulae IMRU 467 using the pSPR910 integration vector to yield the recipient strain SPW910. The self-transmissible nature of pSLV45 was determined by transfer of pSLV45.680 and pSLV45.681 from the donor strains SPW680 and SPW681 into the recipient strain SPW910. Southern analysis indicated the presence of hygromycin-and pSLV45-hybridizing sequences within SPW910 exconjugants. PFGE analysis confirmed pSLV45.680 and pSLV45.681 were transferred intact and formed freely replicating linear plasmids. Sequence analysis of pSPRX604 revealed genes predicted to be involved in plasmid transfer, partitioning and regulation. The transfer of the linear plasmid pSLV45 from S. lavendulae IMRU 3455 into S. lavendulae IMRU 467 may allow the development of pSLV45 as an actinomycete-to-actinomycete conjugative shuttle vector. INTRODUCTIONLinear plasmids with 59 terminally attached proteins (TPs) and terminal inverted repeats (TIRs) have been found in bacteria, plants, filamentous fungi and yeasts, and often within actinomycetes (Griffiths, 1995;Hinnebusch & Tilly, 1993;Meinhardt et al., 1990;Rohe et al., 1992). Actinomycete linear plasmids pSLA1 (17 kb) and pSLA2 (17 kb) were first detected in the lankacidin-producer Streptomyces sp. 7434-AN4 and in Streptomyces rochei, respectively (Hayakawa et al., 1979;Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). Both plasmids were shown to contain TIRs and TPs (Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). With the advent of PFGE analysis, other actinomycete linear plasmids have been identified including Streptomyces lividans SLP2 (50 kb), Streptomyces clavuligerus pSCL1 (12 kb), Streptomyces avermitilis pSA1 and pSA2 (100 kb and 250 kb), Streptomyces rimosus pZG101 (387 kb), Streptomyces lasaliensis pKSL (520 kb) and Streptomyces coelicolor SCP1 (363 kb) Evans et al., 1994;Gravius et al., 1994;Keen et al., 1988;Kinashi & Shimaji, 1987;Kinashi & Shimaji-Murayama, 1991;Kinashi et al., 1994). All these plasmids were found to contain TIRs and TPs. In addition, both pSLA2 and SLP2 were shown to contain an internal origin of replication for bi-directional replication of DNA (Chang & Cohen, 1994;Chang et al., 1996;Huang et al., 2003).Unlike the chromosomes of most bacteria, which are circular in nature, the chromosomes of actinomycetes are linear (Lezhava et al., 1995;Lin et al., 1993). This was first demonstrated in S. lividans and has now been established for several other actinomycetes including S. coelicolor, Streptomyces griseus and Saccharopolyspora erythraea (formerly Streptomyces erythraeus) (Lezhava et al., 1995;Lin et al., 1993;Reeves et al., 1998). All actinomycete chromosomes characterized to da...

show abstract

Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids

Cited by 69 publications

References 13 publications

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

Isolation and partial characterization of a new linear DNA plasmid isolated from Bacillus polymyxa SCE2

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Contact Info

Product

Resources

About