Hydrogels derived from central nervous system extracellular matrix

Medberry, Christopher J.; Crapo, Peter M.; Siu, Bernard F.; Carruthers, Christopher A.; Wolf, Matthew T.; Nagarkar, Shailesh; Agrawal, Vineet; Jones, Kristen E.; Kelly, Jeremy; Johnson, Scott A.; Velankar, Sachin; Watkins, Simon C.; Modo, Michel; Badylak, Stephen F.

doi:10.1016/j.biomaterials.2012.10.062

Cited by 243 publications

(258 citation statements)

References 48 publications

Supporting

Mentioning

246

Contrasting

Unclassified

Order By: Relevance

“…Recent work has described the potential benefits of ECM scaffold materials derived from homologous tissue versus heterologous tissue when used in selected anatomic locations. 2,[4][5][6][7][8][9][10][11] The homologous ECM can preferentially maintain tissuespecific cell phenotypes, [4][5][6][7] promote cell proliferation, 6,8 induce tissue-specific differentiation, 9 and enhance the chemotaxis of stem cells. 2,10,12 However, the preference or necessity for tissue-specific ECM has not been shown for all therapeutic applications.…”

mentioning

confidence: 99%

Tissue-Specific Effects of Esophageal Extracellular Matrix

Keane

DeWard

Londoño

et al. 2015

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 99%

Tissue-Specific Effects of Esophageal Extracellular Matrix

Keane

DeWard

Londoño

et al. 2015

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

“…In cultured N1E-115 neuroblastoma cells, brain ECM was shown to promote neurite growth, an effect that was not evident in cells treated with spinal cord ECM or UBM. 15 ECM derived from the CNS also facilitates the differentiation of NSCs into neurons, while UBM does not have a significant impact. 26 In the present study, we demonstrate the therapeutic effects of brain ECM in an animal model of TBI, with some longterm histological protection and functional improvements.…”

Section: Discussionmentioning

confidence: 99%

Implantation of Brain-derived Extracellular Matrix Enhances Neurological Recovery after Traumatic Brain Injury

2016

Cell Transplant

View full text Add to dashboard Cite

Scaffolds composed of extracellular matrix (ECM) are being investigated for their ability to facilitate brain tissue remodeling and repair following injury. The present study tested the hypothesis that the implantation of brain-derived ECM would attenuate experimental traumatic brain injury (TBI) and explored potential underlying mechanisms. TBI was induced in mice by a controlled cortical impact (CCI). ECM was isolated from normal porcine brain tissue by decellularization methods, prepared as a hydrogel, and injected into the ipsilesional corpus callosum and striatum 1 h after CCI. Lesion volume and neurological function were evaluated up to 35 d after TBI. Immunohistochemistry was performed to assess post-TBI white matter integrity, reactive astrogliosis, and microglial activation. We found that ECM treatment reduced lesion volume and improved neurobehavioral function. ECM-treated mice showed less post-TBI neurodegeneration in the hippocampus and less white matter injury than control, vehicle-treated mice. Furthermore, ECM ameliorated TBI-induced gliosis and microglial pro-inflammatory responses, thereby providing a favorable microenvironment for tissue repair. Our study indicates that brain ECM hydrogel implantation improved the brain microenvironment that facilitates post-TBI tissue recovery. Brain ECM offers excellent biocompatibility and holds potential as a therapeutic agent for TBI, alone or in combination with other treatments.

show abstract

“…Several combination ECM materials isolated from mammalian sources are commercially available [52,77] , while others are in various stages of development and characterization [19,[78][79][80] .…”

Section: Polymersmentioning

confidence: 99%

“…Recently, several groups have started to isolate ECM from CNS to study its potential as a regenerative substrate for SCi [80,85] . Table 2.…”

Section: Polymersmentioning

confidence: 99%

Biomaterials for spinal cord repair

Haggerty

Oudega

2013

Neurosci. Bull.

View full text Add to dashboard Cite

Spinal cord injury (SCi) results in permanent loss of function leading to often devastating personal, economic and social problems. A contributing factor to the permanence of SCi is that damaged axons do not regenerate, which prevents the re-establishment of axonal circuits involved in function. Many groups are working to develop treatments that address the lack of axon regeneration after SCi. The emergence of biomaterials for regeneration and increased collaboration between engineers, basic and translational scientists, and clinicians hold promise for the development of effective therapies for SCi. A plethora of biomaterials is available and has been tested in various models of SCi. Considering the clinical relevance of contusion injuries, we primarily focus on polymers that meet the specific criteria for addressing this type of injury. Biomaterials may provide structural support and/or serve as a delivery vehicle for factors to arrest growth inhibition and promote axonal growth. Designing materials to address the specific needs of the damaged central nervous system is crucial and possible with current technology. Here, we review the most prominent materials, their optimal characteristics, and their potential roles in repairing and regenerating damaged axons following SCi.Keywords: spinal cord injury; axon regeneration; biodegradable materials; extracellular matrix proteins; functional recovery; growth factor; guidance; injury and repair; spinal motor neuron IntroductionSpinal cord injury (SCi) causes loss of neurons and axons resulting in motor and sensory function impairments. There are thousands of new cases of SCi in the world annually [1,2] occurring often in young adults. The lack of endogenous repair and the significant costs to the individual, family and society [1] are important motivations behind the continuing efforts to develop effective therapies. Promoting axonal regeneration is considered a potential repair strategy because it could lead to recovery of axonal circuits involved in motor and/or sensory function [3] .The central nervous system (CNS) neurons are intrinsically capable of some regeneration of damaged axons [4] but their attempts after SCi are frustrated by structural and chemical obstructions in the damaged nervous tissue [5] . Spinal cord repair approaches typically lead to small functional gains. one feature that most of these approaches have in common is a poor axonal regeneration response, suggesting that promoting axonal regeneration, including synapse formation, is essential to achieve substantial functional repair after SCi. if so, it is rational to anticipate that effective spinal cord therapies will need to include interventions addressing the axon growth-inhibition that leads to the poor axonal growth responses. As introduced above, axonal regeneration is considered an important repair mechanism for the injured spinal cord. Therefore, this review focuses on materials that have shown most promise to elicit an axonal regeneration response to foster functional restoration after ...

show abstract

Hydrogels derived from central nervous system extracellular matrix

Cited by 243 publications

References 48 publications

Tissue-Specific Effects of Esophageal Extracellular Matrix

Tissue-Specific Effects of Esophageal Extracellular Matrix

Implantation of Brain-derived Extracellular Matrix Enhances Neurological Recovery after Traumatic Brain Injury

Biomaterials for spinal cord repair

Contact Info

Product

Resources

About