Hydrogel Tissue Adhesive for Sealing Corneal Incisions

Abstract

The purpose of the study was to assess the efficacy of a newly developed hydrogel tissue adhesive for sealing corneal incisions in ex vivo rabbit eyes. Simple through-and-through central linear corneal incisions measuring 1–5 mm were created in enucleated rabbit eyes. As controls, the leaking pressures were measured immediately after the incisions had been made. Afterwards, the incisions were sealed with hydrogel adhesive in group 1 and with a single 10-0 nylon suture in group 2. The leaking pressure of each incision was measured after the wounds had been sealed in both groups. The leaking pressures of the controls and the sealed incisions were compared for statistical significance using the Wilcoxon signed rank test. The difference between the leaking pressures before and after sealing the incisions in groups 1 and 2 were compared using the Mann-Whitney rank sum test. The leaking pressures were significantly higher after sealing in both groups 1 and 2 compared to the controls for all incision sizes. The differences in the leaking pressures before and after adhesive application were higher than before and after suturing in all incision sizes. The differences were all statistically significant except for the 1-mm incisions. We conclude that the hydrogel glue has the potential to be used for sealing corneal incisions measuring up to 5 mm.

Synthesis and characterization of a chondroitin sulfate-polyethylene glycol corneal adhesive

“…The adhesives are classified as biological, 13 synthetic, 14 or a combination of the two [15][16][17] ; while some useful techniques to seal the incision include the use of soldering materials 18 and tissue welding. 19 We previously developed an ocular adhesive based on chondroitin sulfate (CS) 17 but modified the chemistry in the current study to enhance biocompatibility.…”

Seprafilm as a new antifibrotic agent following trabeculectomy in rabbit eyes

“…The pocket was closed without Seprafi lm insertion in the nine rabbits of the non-Seprafi lm group. Following the method of Kalayci et al, 13 we measured the force required to detach the conjunctiva from the sclera with a sphygmomanometer by pumping air through a 27-gauge needle inserted beneath the conjunctiva while the rabbit was under general anesthesia 4 weeks after surgery (Fig. 2).…”

Therapeutic Drugs for Anterior Segment Ocular Surgery

“…Other ophthalmic adhesives are currently under investigation but remain unproven (Miki et al, 2002;Kalayci et al, 2003;Bloom et al, 2003). Miki and colleagues (2002) reported the use of a novel polymer made from hyaluronic acid and methacrylate groups that is laser activated.…”