Hydrogel-magnetic nanoparticles with immobilized l-asparaginase for biomedical applications

Abstract

The association of magnetic nanoparticles, which could be controlled by a magnetic field and have dimensions which facilitate their penetration in cells/tissues, with hydrogel type biopolymeric shells confer them compatibility and the capacity to retain and deliver bioactive substances. The main objective of this work is the development of a new system based on a biocompatible polymer with organic-inorganic structure capable of vectoring support for biologic active agents (L: -asparaginase, e.g.). Characterization of size and morphology of the hydrogel-magnetic nanoparticles with entrapped L: -asparaginase was made using Dynamic Light Scattering method, Transmission Electron Microscopy and Confocal Microscopy. The structure of magnetic nanoparticles coated with hydrogel was characterized by Fourier Transformed Infrared Spectroscopy. The cytotoxicity of nanoparticles was evaluated and also the interactions with microorganisms. We obtained hydrogel-magnetic nanoparticles with L: -asparaginase entrapped, with sizes below 30 nm in dried stage, capable to penetrate the cells and tissues.

Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha, India

“…Preventase and acrylaway produced from Aspergillus niger and Aspergillus oryzae respectively are commercially available LAse currently used in food industry [7] . LAse is a model enzyme for the development of new drug delivery system [8] , L-asparagine biosensor for leukemia [9] . LAse like glutaminase and urease also plays an important role in the biogeocycling of carbon and nitrogen in natural waters and sediments [10] .…”

“…The results of the morphological, biochemical and physiological tests were put into Bergey's Manual of Determinative Bacteriology [19] and bacterial identification software's like PIBwin version 2.0 and ABIS online for accurate identification. The isolate TPS-12 was exposed to temperature range of 40-100°C, pH range of [3][4][5][6][7][8][9][10][11][12] and sodium chloride concentrations range of 2%-10% for evaluation of physiological characteristics. The antibiogram pattern of the isolate TPS-12 was tested for sensitivity against different antibiotic discs (Himedia, Mumbai) viz., erythromycin (15 …”

“…The isolate TPS-12 when exposed to temperature range of 40-100 °C, showed growth in the temperature range of 150-70 °C, while the growth was restricted above and below the above specified range. The isolate TPS-12 when exposed to different pH range (3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and tolerance to different concentrations of sodium chloride (2%-10%), exhibited growth at pH range of [5][6][7][8][9][10] and tolerated sodium chloride concentrations 2%-7% respectively. The isolate showed no growth at pH 3 The results of the antibiogram pattern revealed that the isolate TPS-12 was very sensitive to all the tested antibiotics.…”

“…The enzyme inhibited the growth of the two human cell lines including hepatocellular carcinoma and colon carcinoma with IC 50 value of 8.38 µg/mL and 4.67 µg/mL, respectively [3] . It is also used for the treatment of pancreatic carcinoma.…”

Assessment of Physical Process Conditions for Enhanced Production of Novel Glutaminase-Free L-Asparaginase from Pectobacterium carotovorum MTCC 1428

“…Therefore, glutaminase-free L-asparaginase is essentially required for successful clinical studies. Moreover, L-asparaginase is also used in food industry for the production of acrylamidefree food [5], model enzyme for the development of new drug delivery system [6], and L-asparagine biosensor for leukemia [7]. L-asparaginase enzymatically cleaves Lasparagine (amino acid essential for lymphoblasts' growth) to ammonia and L-aspartic acid.…”