Hydrogel glycan microarrays

Dyukova, V.I.; Dementieva, Ekaterina; Zubtsov, D.A.; Galanina, Oxana; Bovin, Nicolai V.; Rubina, A. Yu.

doi:10.1016/j.ab.2005.09.009

Cited by 61 publications

(40 citation statements)

References 28 publications

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“…For example, it was reported that an ordinary microarray scanner can collect signals in a scope from the substrate surface to a height of more than 20 µm, so the particles with the size between tens and several hundred of nanometers should be suitable for the microarray analysis. 8,32 The fluorescence image (Figure 7) scanned by a confocal microarray scanner was an early test to use the carbohydrate affinity particles in this field. Though this analysis gave some meaningful results, presently, it still lacked reliability and accuracy comparing with the former protein binding experiment.…”

Section: 62mentioning

confidence: 99%

“…8,32,38 In this study, a route for preparation of hydrazide-activated small particles from PAN was designed and synthesized, the unmodified carbohydrates were directly coated onto the particles in very gentle conditions, and then a simple test was applied to examine the biological affinity of the resultant carbohydrate coated particles.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the polymer hydrogel particles were also used in fabrication of the three dimensional (3D) carbohydrate chips. 32 A problem restricting the application possibilities of the chemical attachment method is that it usually required a multi-step modification of the carbohydrate molecule with a chemical active ligand, [26][27][28][29][30][31] however a rigorous modification condition would to some extent destroy the carbohydrates' original structure, especially to many bioactive carbohydrates that are highly sulfated, phosphated or substituted with other sensitive functional groups. Therefore investigators have made great efforts to devise the novel routes and materials which can directly attach the unmodified carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate Coated Polymer Particles: Preparation and Protein‐binding Studies

et al. 2011

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A facile and effective organic synthesis route was provided for preparation of the carbohydrate coated polymer small particles. First, amino-activated particles were prepared from PAN (polyacrylonitrile) by the heterogeneous cross-linking method, then the amino groups on the particle were converted into hydrazide groups by N-alkylation and hydrazine activation, meanwhile the length of linker was prolonged to the designed value. FTIR (Fourier Transform Infrared Spectroscopy) was used to study the reactions in the synthesis steps and to optimize part of the synthesis conditions. Two carbohydrates (maltose and heparin) were then directly coupled to the hydrazide-activated particles in a relative high yield, the obtained particles were used to interact with BSA (bovine serum albumin) and typical heparin-binding proteins. All of these particles had some nonspecific interactions with proteins and heparin coated particles showed additional strong binding ability with the heparin-binding proteins. Data also showed that a longer linker length not only weakened nonspecific interaction but also increased the specific binding ability. As the carbohydrate coated particles are independent, flexible and easily detectable, they should be good acceptors for the diverse bioactive studies of carbohydrates.

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Section: 62mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carbohydrate Coated Polymer Particles: Preparation and Protein‐binding Studies

et al. 2011

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“…Although it is known that hydrogels can be porous [15,22] it is difficult to predict its uniformity within the HC-PCF, even if the same rule-of-thumb applies. Figure 4c, for example, shows a scanning electron microscope (SEM) image of the HC-PCF 1550 fibre with hydrogel partly formed in the core.…”

Section: Hydrogel Filled Hc-pcfmentioning

confidence: 99%

“…Lately, three dimensional (3D) hydrogels have been demonstrated as alterative substrates for microarray applications [15] with higher sensitivity [16,17] than the 2D matrices when grown on glass substrates. Hydrogels (also called aquagels) are mostly liquid, yet behave like solids due to a threedimensional crosslinked network within the lattice structure.…”

Section: Introductionmentioning

confidence: 99%

Integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre for DNA probe immobilization

Rutowska

Gunning

Kivlehan

et al. 2010

Meas. Sci. Technol.

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Abstract. In this paper, we demonstrate the integration of a 3D hydrogel matrix within a hollow core Photonic Crystal Fibre (HC-PCF). In addition we also show the fluorescence of Cy5-labelled DNA molecules immobilised within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino-groups of biomolecules using an appropriate cross-linker, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, can be designed to maximise the capture of fluorescence to improve sensitivity and provide longer interaction lengths.This could enable the development of fibre-based point-of-care and remote systems, where the enhanced sensitivity would relax the constraints placed on sources and detectors. In this paper, we will discuss the formation of such polyethylene glycol diacrylate (PEGDA) hydrogels within a HC-PCF, including their optical properties such as light-propagation and auto-fluorescence.

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Glycans: Array‐Based Techniques

Oyelaran

Manimala

Gildersleeve

2008

Wiley Encyclopedia of Chemical Biology

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Glycan arrays are powerful tools for high throughput analysis of carbohydrate–macromolecule interactions. A glycan array is composed of many different carbohydrate structures immobilized on a solid support in an orderly arrangement. This review describes the challenges and considerations in the development of a glycan array. Various array formats, immobilization techniques, and assay systems are discussed. In addition, several interesting applications of glycan array technology are described, such as assessing the specificities of carbohydrate‐binding lectins and antibodies, profiling antiglycan antibodies in serum as biomarkers of disease, and evaluating carbohydrate‐dependent cell binding.

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Hydrogel glycan microarrays

Cited by 61 publications

References 28 publications

Carbohydrate Coated Polymer Particles: Preparation and Protein‐binding Studies

Carbohydrate Coated Polymer Particles: Preparation and Protein‐binding Studies

Integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre for DNA probe immobilization

Glycans: Array‐Based Techniques

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