Hydrogel-coating improves the in-vivo stability of electrochemical aptamer-based biosensors

Li, Shaoguang; Dai, Jun; Zhu, Man; Arroyo‐Currás, Netzahualcóyotl; Li, Hongxing; Wang, Yuanyuan; Wang, Quan; Lou, Xiaoding; Kippin, Tod E.; Wang, Shixuan; Li, Hui; Xia, Fan

doi:10.1101/2020.11.15.383992

Cited by 9 publications

(13 citation statements)

References 42 publications

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“…developed hydrogel‐coated biosensors for aptamer‐based sensing electrodes to improve the stability as it may aid in deployment to complex biological environments like in veins, muscle tissue, bladder cells, or tumor microenvironments ( Figure a,b). [ 162 ] Of particular note, Li et al. affirmed that drift corrections following implantation are needed to account for the placement of the implant within a living and moving animal model, and argue for real‐time sensing potential given that the equilibration time is less than 1 min for their gel‐protected aptamer probe.…”

Section: In Vivo Sensorsmentioning

confidence: 99%

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Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Shafique

Vries

Strauss

et al. 2022

Adv Healthcare Materials

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Novel biomaterials for bio‐ and chemical sensing applications have gained considerable traction in the diagnostic community with rising trends of using biocompatible and lowly cytotoxic material. Hydrogel‐based electrochemical sensors have become a promising candidate for their swellable, nano‐/microporous, and aqueous 3D structures capable of immobilizing catalytic enzymes, electroactive species, whole cells, and complex tissue models, while maintaining tunable mechanical properties in wearable and implantable applications. With advances in highly controllable fabrication and processability of these novel biomaterials, the possibility of bio‐nanocomposite hydrogel‐based electrochemical sensing presents a paradigm shift in the development of biocompatible, “smart,” and sensitive health monitoring point‐of‐care devices. Here, recent advances in electrochemical hydrogels for the detection of biomarkers in vitro, in situ, and in vivo are briefly reviewed to demonstrate their applicability in ideal conditions, in complex cellular environments, and in live animal models, respectively, to provide a comprehensive assessment of whether these biomaterials are ready for point‐of‐care translation and biointegration. Sensors based on conductive and nonconductive polymers are presented, with highlights of nano‐/microstructured electrodes that provide enhanced sensitivity and selectivity in biocompatible matrices. An outlook on current challenges that shall be addressed for the realization of truly continuous real‐time sensing platforms is also presented.

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Section: In Vivo Sensorsmentioning

confidence: 99%

“…Implanted aptamer-based electrochemical biosensors to detect the presence of drug concentrations in circulation for a) unprotected sensors and b) hydrogel-protected sensors, showing a sensitive, accurate and reproducible response from the live model's jugular vein implant. Reproduced with permission [162]. Copyright 2020, bioRxiv.…”

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confidence: 99%

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Shafique

Vries

Strauss

et al. 2022

Adv Healthcare Materials

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“…This is presumably due to the instability of E-AB sensors in vivo that results from the degradation of the recognition aptamer, and non-specific interferant adsorption on the sensor's surface may cause significant drift [154]. Nevertheless, in vivo work describing the detection of cocaine or small drug molecules has been reported [95,155,156] and methods such as hydrogel coating [157], kinetic differential measurement [158], and dual-reporter approaches [159] have been explored to correct drifted signal. [147].…”

Section: Aptamer Biosensorsmentioning

confidence: 99%

Recent Advances in In Vivo Neurochemical Monitoring

Tan

Robbins

et al. 2021

Micromachines

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The brain is a complex network that accounts for only 5% of human mass but consumes 20% of our energy. Uncovering the mysteries of the brain’s functions in motion, memory, learning, behavior, and mental health remains a hot but challenging topic. Neurochemicals in the brain, such as neurotransmitters, neuromodulators, gliotransmitters, hormones, and metabolism substrates and products, play vital roles in mediating and modulating normal brain function, and their abnormal release or imbalanced concentrations can cause various diseases, such as epilepsy, Alzheimer’s disease, and Parkinson’s disease. A wide range of techniques have been used to probe the concentrations of neurochemicals under normal, stimulated, diseased, and drug-induced conditions in order to understand the neurochemistry of drug mechanisms and develop diagnostic tools or therapies. Recent advancements in detection methods, device fabrication, and new materials have resulted in the development of neurochemical sensors with improved performance. However, direct in vivo measurements require a robust sensor that is highly sensitive and selective with minimal fouling and reduced inflammatory foreign body responses. Here, we review recent advances in neurochemical sensor development for in vivo studies, with a focus on electrochemical and optical probes. Other alternative methods are also compared. We discuss in detail the in vivo challenges for these methods and provide an outlook for future directions.

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“…Another important factor is that a large number of microorganisms on the surface of the skin can enter the body easily and cause infection, and damage to the glucose oxidase coating results in abnormal test values. Shaoguang Li’s team used hydrogel to coat the enzyme [ 5 ], which was shown to protect the enzyme layer and prolong the effective time of detection in an in vivo experiment on rats.…”

Section: Introductionmentioning

confidence: 99%

Continuous Glucose Monitoring System Based on Percutaneous Microneedle Array

et al. 2022

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A continuous blood glucose monitoring system (CGMS) which include a microneedle-array blood glucose sensor, a circuit module, and a transmission module placed in a wearable device is developed in this research. When in use, the wearable device is attached to the human body with the microneedle array inserted under the skin for continuous blood glucose sensing, and the measured signals are transmitted wirelessly to a mobile phone or computer for analysis. The purpose of this study is to replace the conventionally used method of puncture for blood collection and test strips are used to measure the blood glucose signals. The microneedle sensor of this CGMS uses a 1 mm length needle in a 3 mm × 3 mm microneedle array for percutaneous minimally invasive blood glucose measurement. This size of microneedle does not cause bleeding damage to the body when used. The microneedle sensor is placed under the skin and their solutions are discussed. The blood glucose sensor measured the in vitro simulant fluid with a glucose concentration range of 50~400 mg/dL. In addition, a micro-transfer method is developed to accurately deposit the enzyme onto the tip of the microneedle, after which cyclic voltammetry (CV) is used to measure the glucose simulation solution to verify whether the difference in the amount of enzyme on each microneedle is less than 10%. Finally, various experiments and analyses are carried out to reduce the size of the device, test effective durability (approximately 7 days), and the feasibility of minimally invasive CGMS is evaluated by tests on two persons.

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Hydrogel-coating improves the in-vivo stability of electrochemical aptamer-based biosensors

Cited by 9 publications

References 42 publications

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Recent Advances in In Vivo Neurochemical Monitoring

Continuous Glucose Monitoring System Based on Percutaneous Microneedle Array

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