Hydrogel Bioinks of Alginate and Curcumin-Loaded Cellulose Ester-Based Particles for the Biofabrication of Drug-Releasing Living Tissue Analogs

Abstract

3D bioprinting is a versatile technique that allows the fabrication of living tissue analogs through the layer-by-layer deposition of cell-laden biomaterials, viz. bioinks. In this work, composite alginate hydrogel-based bioinks reinforced with curcumin-loaded particles of cellulose esters (CEpCUR) and laden with human keratinocytes (HaCaT) are developed. The addition of the CEpCUR particles, with sizes of 740 ± 147 nm, improves the rheological properties of the inks, increasing their shear stress and viscosity, while preserving the recovery rate and the mechanical and viscoelastic properties of the resulting fully cross-linked hydrogels. Moreover, the presence of these particles reduces the degradation rate of the hydrogels from 26.3 ± 0.8% (ALG) to 18.7 ± 1.3% (ALG:CEpCUR_10%) after 3 days in the culture medium. The 3D structures printed with the ALG:CEpCUR inks reveal increased printing definition and the ability to release curcumin (with nearly 70% of cumulative release after 24 h in PBS). After being laden with HaCaT cells (1.2 × 10 6 cells mL −1 ), the ALG:CEpCUR bioinks can be successfully 3D bioprinted, and the obtained living constructs show good dimensional stability and high cell viabilities at 7 days post-bioprinting (nearly 90%), confirming their great potential for application in fields like wound healing.

3D skin bioprinting as promising therapeutic strategy for radiation‐associated skin injuries

“…Limited by ethical rules and experimental conditions, most laboratory studies on 3D-bioprinted skin constructions use cell lines as seeded cells, 41,43,44 which are obviously not suitable for clinical applications. Many companies of skin tissue engineering products choose allogeneic fetal keratinocytes and skin fibroblasts as seeded cells in their products, 16 which is also one choice for 3D-bioprinted skin substitutes.…”

Multifunctional Cellulose Nanofibrils–GdF3 Nanoparticles Hybrid Gel and Its Potential Uses for Drug Delivery and Magnetic Resonance Imaging

“…In recent years, worldwide focus on the employment of nanocellulose appears in the field of biomedical applications, which includes drug delivery systems, ,− wound dressing, − scaffolds, − etc ., along with the assistance of the coupling of multidisciplinary fields such as biology and chemistry . Durand et al provided a comprehensive overview of the application and the health and toxicology concerns of nanocellulose in the biomedical field .…”