Hydrogel-based dressings in the treatment of partial thickness experimentally induced burn wounds in rats

Bernardes, Milton Junio Cândido; Gonçalves, Randys Caldeira; Carvalho, Carolyna de Sousa; Rosa, Luciana Martins; Ferreira, Amanda Peixoto; Vilela, Marielle Sousa; Vinaud, Marina Clare; Júnior, Hélio Galdino; Júnior, Ruy de Souza Lino

doi:10.1590/acb370401

Cited by 3 publications

(3 citation statements)

References 54 publications

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“…Similarly, the alginate/pectin film in combination with modified chitosan/curcumin NPs reported comparable results at Day 18 [ 70 ], 15 days for Calendula officinalis [ 71 ], 82% of wound reduction at Day 14 for piperine carbopol hydrogels mixed with Aloe vera [ 72 ], and for chitosan hydrogels with Aloe vera , wounds heal after 13 days [ 71 , 72 ]. Other works, using commercial products such as Restaured ® , based on Aloe vera , showed almost 92% of reduction wound around Day 15 [ 31 ], and Debrigel™, based on alginate, sodium carboxymethyl cellulose, and propylene glycol, exhibited 92% of contraction of partial thickness at Day 15; however, this last product was tested in burn wounds [ 73 ]. It is worth saying that none of these results report the presence of sweat glands or follicles.…”

Section: Discussionmentioning

confidence: 99%

Characterization and In Vivo Assay of Allantoin-Enriched Pectin Hydrogel for the Treatment of Skin Wounds

Saucedo-Acuña

Meza-Valle

Cuevas-González

et al. 2023

IJMS

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This work describes a liquid allantoin-enriched pectin hydrogel with hydrophilic behavior that is supported by the presence of functional groups related to healing efficacy. A topical study shows the effect of the hydrogel application on surgically induced skin wound healing in a rat model. Contact angle measurements confirm hydrophilic behavior (11.37°), while Fourier-transform infrared spectroscopy indicates the presence of functional groups related to the healing effectiveness (carboxylic acid and amine groups). Allantoin is distributed on the surface and inside the amorphous pectin hydrogel surrounded by a heterogeneous distribution of pores. This promotes wound drying with better interaction between the hydrogel and cells involved in the wound healing process. An experimental study with female Wistar rats indicates that the hydrogel improves wound contraction, reducing around 71.43% of the total healing time and reaching total wound closure in 15 days.

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Section: Discussionmentioning

confidence: 99%

Characterization and In Vivo Assay of Allantoin-Enriched Pectin Hydrogel for the Treatment of Skin Wounds

Saucedo-Acuña

Meza-Valle

Cuevas-González

et al. 2023

IJMS

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“…Also of scientific value is the study of the possibility of using hydrogels saturated with various drugs for local wound treatment [3,4].…”

Section: морфологія / Morphologymentioning

confidence: 99%

“…Також, наукову цінність представляє собою вивчення можливості застосування гідрогелів насиче-них різними лікарськими препаратами для місцевого лікування ран [3,4].…”

Section: вступunclassified

Morphological Assessment of the Application of Hydrogels for the Local Treatment of Inflammatory Wounds in an Experiment

Slobodian

2023

VPBM

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Вісник проблем біології і медицини -2023 -Вип. 4 (171) / Bulletin of problems in biology and medicine -2023 -Issue 4 (171) 366 МОРФОЛОГІЯ / MORPHOLOGYsurements of the heigh and width of folds of mucosa, the thikness of lamina propria, as well as the thikness of mucosa between folds were made. For this purpose pictures of histological preparations were used, taken using a х20, х40 lens. The confidence level for morphometric studies was set at &=95. The difference between groups was considered significant when p˂0,05. All statistical calculations were performed using the software RStudio v. 1.2.5042.Results. It was found that after two weeks of experiment, the thikness of mucosa increases slightly to (14,40±2,02) μm, after 4 weeks of nalbuphine administration it is (18.40±3.60) μm, and after 6 weeks it is equal to (23,20±8.54) μm. After 2 weeks of the experiment, the thickness of the lamina propria of the mucosa also increases to (9.28±1.47) μm, after 4 weeks of nalbuphine administration it is (13.40±3.74) μm, and after 6 weeks is equal to (16.00±3.59) μm. After 2 weeks of administration of nalbuphine, there is a decrease in the length of the fold of the mucosa of the ampulla of the uterine tube of a white rat, compared to the control, to (113.00±38.60) μm, the control -(143.00±57.80) μm, p<0 ,05, after 4 weeks of nalbuphine administration it is (92.00±47.60) μm, after 6 weeks it is (53.60±29.90) μm. After 2 weeks of the experiment, the width of the fold of the mucosa also decreases to (53,00±18.80) μm, the control -(60.90±18.40) μm, p<0.05, after 4 weeks of nalbuphine administration it is (40.20±15.60) μm, and after 6 weeks it is equal to (32.10±16.10) μm.

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Acceleration of wound healing using adipose mesenchymal stem cell secretome hydrogel on partial-thickness cutaneous thermal burn wounds: An in vivo study in rats

Kuncorojakti,

Pratama,

Antujala

et al. 2024

Vet World

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Background and Aim: The intricate healing process involves distinct sequential and overlapping phases in thermal injury. To maintain the zone of stasis in Jackson’s burn wound model, proper wound intervention is essential. The extent of research on the histoarchitecture of thermal wound healing and the application of mesenchymal stem cell (MSC)-free-based therapy is limited. This study aimed to assess the efficacy of MSC-secretome-based hydrogel for treating partial-thickness cutaneous thermal burn wounds. Materials and Methods: Eighteen male Wistar rats were divided into three groups, namely the hydrogel base (10 mg), hydrogel secretome (10 mg) and Bioplacenton™ (10 mg) treatment groups. All groups were treated twice a day (morning and evening) for 7 days. Skin tissue samples from the animals were processed for histological evaluation using the formalin-fixed paraffin-embedded method on days 3 and 7. Results: This study’s findings showed that secretome hydrogel expedited thermal burn wound healing, decreasing residual burn area, boosting collagen deposition and angiogenesis, guiding scar formation, and influencing the inflammation response facilitated by polymorphonuclear leukocytes and macrophages. Conclusion: The secretome hydrogel significantly improves healing outcomes in partial-thickness cutaneous thermal burn wounds. The administration of secretome hydrogel accelerates the reduction of the residual burn area and promotes fibroblast proliferation and collagen density. The repairment of histo-architecture of the damaged tissue was also observed such as the reduction of burn depth, increased angiogenesis and epidermal scar index while the decreased dermal scar index. Furthermore, the secretome hydrogel can modulate the immunocompetent cells by decreasing the polymorphonuclear and increasing the mononuclear cells. Thus, it effectively and safely substitutes for thermal injury stem cell-free therapeutic approaches. The study focuses on the microscopical evaluation of secretome hydrogel; further research to investigate at the molecular level may be useful in predicting the beneficial effect of secretome hydrogel in accelerating wound healing. Keywords: drug safety, mesenchymal stem cell-conditioned media, stem cell-free-therapy, thermal injury, wound healing.

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Hydrogel-based dressings in the treatment of partial thickness experimentally induced burn wounds in rats

Cited by 3 publications

References 54 publications

Characterization and In Vivo Assay of Allantoin-Enriched Pectin Hydrogel for the Treatment of Skin Wounds

Characterization and In Vivo Assay of Allantoin-Enriched Pectin Hydrogel for the Treatment of Skin Wounds

Morphological Assessment of the Application of Hydrogels for the Local Treatment of Inflammatory Wounds in an Experiment

Acceleration of wound healing using adipose mesenchymal stem cell secretome hydrogel on partial-thickness cutaneous thermal burn wounds: An in vivo study in rats

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