Hydrogel-Assisted Electrospinning for Fabrication of a 3D Complex Tailored Nanofiber Macrostructure

Eom, Seongsu; Park, Sang Min; Hong, Hyunsuk; Kwon, Jung Ah; Oh, Sang–Rok; Kim, Junesun; Kim, Dong Sung

doi:10.1021/acsami.0c14438

Cited by 43 publications

(48 citation statements)

References 69 publications

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“… 3 , 19 Thus, cell-frameworks based on EMC-like electrospun scaffolds have been extensively studied due to their great capacity to promote cell adhesion, proliferation, and tissue formation. 3 , 19 …”

Section: Introductionmentioning

confidence: 99%

Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

Deus

França

Forero

et al. 2021

ACS Appl. Mater. Interfaces

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The ability of mimicking the extracellular matrix architecture has gained electrospun scaffolds a prominent space into the tissue engineering field. The high surface-to-volume aspect ratio of nanofibers increases their bioactivity while enhancing the bonding strength with the host tissue. Over the years, numerous polyesters, such as poly(lactic acid) (PLA), have been consolidated as excellent matrices for biomedical applications. However, this class of polymers usually has a high hydrophobic character, which limits cell attachment and proliferation, and therefore decreases biological interactions. In this way, functionalization of polyester-based materials is often performed in order to modify their interfacial free energy and achieve more hydrophilic surfaces. Herein, we report the preparation, characterization, and in vitro assessment of electrospun PLA fibers with low contents (0.1 wt %) of different curcuminoids featuring π-conjugated systems, and a central β-diketone unit, including curcumin itself. We evaluated the potential of these materials for photochemical and biomedical purposes. For this, we investigated their optical properties, water contact angle, and surface features while assessing their in vitro behavior using SH-SY5Y cells. Our results demonstrate the successful generation of homogeneous and defect-free fluorescent fibers, which are noncytotoxic, exhibit enhanced hydrophilicity, and as such greater cell adhesion and proliferation toward neuroblastoma cells. The unexpected tailoring of the scaffolds’ interfacial free energy has been associated with the strong interactions between the PLA hydrophobic sites and the nonpolar groups from curcuminoids, which indicate its role for releasing hydrophilic sites from both parts. This investigation reveals a straightforward approach to produce photoluminescent 3D-scaffolds with enhanced biological properties by using a polymer that is essentially hydrophobic combined with the low contents of photoactive and multifunctional curcuminoids

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Section: Introductionmentioning

confidence: 99%

Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

Deus

França

Forero

et al. 2021

ACS Appl. Mater. Interfaces

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“…These scaffolds presented larger pore sizes and increased porosity compared to traditionally electrospun random and aligned scaffolds, which resulted in greater tendon cell penetration, proliferation, and gene expression of tendon-specific markers. A recent study [ 313 ] has described the generation of 3D electrospun structures using specialised hydrogel collectors, which can be either removed post-spinning, forming hollow structures (e.g., for blood vessel TE), or maintained, resulting in hydrogel scaffolds lined with electrospun fibres. Importantly, drug/cell-loaded hydrogels can be used as collectors and thus biological delivery systems can be developed using this technique.…”

Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Gonçalves¹,

Moreira²,

Weber

et al. 2021

Pharmaceutics

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The socioeconomic impact of osteochondral (OC) damage has been increasing steadily over time in the global population, and the promise of tissue engineering in generating biomimetic tissues replicating the physiological OC environment and architecture has been falling short of its projected potential. The most recent advances in OC tissue engineering are summarised in this work, with a focus on electrospun and 3D printed biomaterials combined with stem cells and biochemical stimuli, to identify what is causing this pitfall between the bench and the patients’ bedside. Even though significant progress has been achieved in electrospinning, 3D-(bio)printing, and induced pluripotent stem cell (iPSC) technologies, it is still challenging to artificially emulate the OC interface and achieve complete regeneration of bone and cartilage tissues. Their intricate architecture and the need for tight spatiotemporal control of cellular and biochemical cues hinder the attainment of long-term functional integration of tissue-engineered constructs. Moreover, this complexity and the high variability in experimental conditions used in different studies undermine the scalability and reproducibility of prospective regenerative medicine solutions. It is clear that further development of standardised, integrative, and economically viable methods regarding scaffold production, cell selection, and additional biochemical and biomechanical stimulation is likely to be the key to accelerate the clinical translation and fill the gap in OC treatment.

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“…It permits the production of extracellular-matrix-like three-dimensional structures with nanoscale fibers which have various advantageous characteristics, such as large area-to-volume ratio and high porosity. 19 In this study, a blend of GO and PCL was first used to generate the outer scaffold (GO-PCL). It should be noted that the use of different concentration of GO affects the mechanical properties and biocompatibility of the final scaffold.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

Liu

Hou

Liu

et al. 2021

IJN

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Background Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall. Materials and Methods In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). To expand the bio-functions (angiogenesis/reducing reactive oxygen species) of the patch (GO-PCL/NAC-CS-PCL), N-acetylcysteine (NAC) was loaded for the repair of full-thickness abdominal wall defects (2×1.5cm) in rat model. Results The double-layered patch (GO-PCL/NAC-CS-PCL) showed excellent mechanical strength and biocompatibility. After 2 months, rats treated with the patch exhibited the desired repair effect with no hernia formation, less adhesion (adhesion score: 1.50±0.50, P<0.001) and more collagen deposition (percentage of collagen deposition: 34.94%±3.31%, P<0.001). Conclusion The double-layered nanomembranes presented in this study have good anti-hernia and anti-adhesion effects, as well as improve the microenvironment in vivo. It, therefore, holds good prospects for the repair of abdominal wall defects and provides a promising key as a postoperative anti-adhesion agent.

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Hydrogel-Assisted Electrospinning for Fabrication of a 3D Complex Tailored Nanofiber Macrostructure

Cited by 43 publications

References 69 publications

Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

Curcuminoid-Tailored Interfacial Free Energy of Hydrophobic Fibers for Enhanced Biological Properties

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

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