Hydrogel as a bioactive material to regulate stem cell fate

Tsou, Yung Hao; Khoneisser, Joe; Huang, Ping; Xu, Xiaoyang

doi:10.1016/j.bioactmat.2016.05.001

Cited by 232 publications

(189 citation statements)

References 152 publications

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“…HA only hydrogels evaluated did not support hMSC growth, as expected, due to its low mechanical strength and anti-adhesive properties [61–63]. HA-based hydrogels are often combined with small peptides or other polymers to enhance cell adhesion and proliferation through bioactive or mechanical cues [54, 61, 64].…”

Section: Discussionmentioning

confidence: 93%

Enzymatically crosslinked silk-hyaluronic acid hydrogels

et al. 2017

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In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues. The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization. Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly. To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity. The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration. In addition, the hydrogels were characterized by enzymatic degradation and for cytotoxicity. Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization. These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.

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Section: Discussionmentioning

confidence: 93%

Enzymatically crosslinked silk-hyaluronic acid hydrogels

et al. 2017

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“…Stem cells or induced pluripotent cells (iPSCs) in microcapsules interact with a 3D ECM which could trigger biochemical and biomechanical cues to promote or inhibit specific differentiation routes 177 . For example, human neural stem cells encapsulated in the core of matrigel core-alginate shell microcapsules or mouse embryonic carcinoma cells in alginate hydrogel beads are prone to neuron differentiation (Fig.…”

Section: Hydrogel Microencapsulation For 3d Cell Culturementioning

confidence: 99%

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Huang

et al. 2017

Lab Chip

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Hydrogel microcapsules provide miniaturized and biocompatible niches for three-dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based microfluidics with tunable size, morphology, and biochemical properties. Therefore, microfluidic generation and manipulation of cell-laden microcapsules can be used for 3D cell culture to mimic the in vivo environment towards applications in tissue engineering and high throughput drug screening. In this review of recent advances mainly since 2010, we will first introduce general characteristics of droplet-based microfluidic devices for cell encapsulation with an emphasis on the fluid dynamics of droplet breakup and internal mixing as they directly influence microcapsule’s size and structure. We will then discuss two on-chip manipulation strategies: sorting and extraction from oil into aqueous phase, which can be integrated into droplet-based microfluidics and significantly improve the qualities of cell-laden hydrogel microcapsules. Finally, we will review various applications of hydrogel microencapsulation for 3D in vitro culture on cell growth and proliferation, stem cell differentiation, tissue development, and co-culture of different types of cells.

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“…[12] Although there are various types of biocompatible and injectable stem cell scaffolds such as poly(lactic acid) derivate, [13] hyaluronic acid, [14] and supramolecular hydrogel, [15] the controlled differentiation of stem cells is of foremost importance to the attainment of the desired tissue construction. [12] Although there are various types of biocompatible and injectable stem cell scaffolds such as poly(lactic acid) derivate, [13] hyaluronic acid, [14] and supramolecular hydrogel, [15] the controlled differentiation of stem cells is of foremost importance to the attainment of the desired tissue construction.…”

Section: Introductionmentioning

confidence: 99%

Fine‐Tunable and Injectable 3D Hydrogel for On‐Demand Stem Cell Niche

2019

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Stem‐cell‐based tissue engineering requires increased stem cell retention, viability, and control of differentiation. The use of biocompatible scaffolds encapsulating stem cells typically addresses the first two problems. To achieve control of stem cell fate, fine‐tuned biocompatible scaffolds with bioactive molecules are necessary. However, given that the fine‐tuning of stem cell scaffolds is associated with UV irradiation and in situ scaffold gelation, this process is in conflict with injectability. Herein, a fine‐tunable and injectable 3D hydrogel system is developed with the use of thermosensitive poly(organophosphazene) bearing β‐cyclodextrin (β‐CD PPZ) and two types of adamantane‐peptides (Ad‐peptides) that are associated with mesenchymal stem cell (MSC) differentiation and that serve as stoichiometrically controlled pendants for fine‐tuning. Given that complexation of hosts and guests subject to strict stoichiometric control is achieved with simple mixing, these fabricated hydrogels exhibit well‐aligned, fine‐tuning responses, even in living animals. Injection of MSCs in fine‐tuned hydrogels also results in various chondrogenic differentiation levels at three weeks postinjection. This is attributed to the differential controls of Ad‐peptides, if MSC preconditioning is excluded. Eventually, the fine‐tunable and injectable 3D hydrogel could be applied as platform technology by simply switching the types of peptides bearing adamantane and their stoichiometry.

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Hydrogel as a bioactive material to regulate stem cell fate

Cited by 232 publications

References 152 publications

Enzymatically crosslinked silk-hyaluronic acid hydrogels

Enzymatically crosslinked silk-hyaluronic acid hydrogels

Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

Fine‐Tunable and Injectable 3D Hydrogel for On‐Demand Stem Cell Niche

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