Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part I. A theoretical approach

Håkansson, Andréas; Magnusson, Emma; Bergenståhl, Björn; Nilsson, Lars

doi:10.1016/j.chroma.2012.07.029

Cited by 61 publications

(42 citation statements)

References 18 publications

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“…The molar mass ( M ) and the gyration radius ( R g ) were obtained from the light scattering data using the Berry method69. The hydrodynamic diameter ( D h ) was determined based on the retention time50.…”

Section: Methodsmentioning

confidence: 99%

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Structural and magnetic properties of multi-core nanoparticles analysed using a generalised numerical inversion method

Bender

Bogart

Posth

et al. 2017

Sci Rep

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The structural and magnetic properties of magnetic multi-core particles were determined by numerical inversion of small angle scattering and isothermal magnetisation data. The investigated particles consist of iron oxide nanoparticle cores (9 nm) embedded in poly(styrene) spheres (160 nm). A thorough physical characterisation of the particles included transmission electron microscopy, X-ray diffraction and asymmetrical flow field-flow fractionation. Their structure was ultimately disclosed by an indirect Fourier transform of static light scattering, small angle X-ray scattering and small angle neutron scattering data of the colloidal dispersion. The extracted pair distance distribution functions clearly indicated that the cores were mostly accumulated in the outer surface layers of the poly(styrene) spheres. To investigate the magnetic properties, the isothermal magnetisation curves of the multi-core particles (immobilised and dispersed in water) were analysed. The study stands out by applying the same numerical approach to extract the apparent moment distributions of the particles as for the indirect Fourier transform. It could be shown that the main peak of the apparent moment distributions correlated to the expected intrinsic moment distribution of the cores. Additional peaks were observed which signaled deviations of the isothermal magnetisation behavior from the non-interacting case, indicating weak dipolar interactions.

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Section: Methodsmentioning

confidence: 99%

“…We observe that the particle diameter is polydisperse and lies in the range ~100–150 nm. The hydrodynamic size distribution of the multi-core particles was determined by AF4-MALS4950.…”

Section: Samplementioning

confidence: 99%

Structural and magnetic properties of multi-core nanoparticles analysed using a generalised numerical inversion method

Bender

Bogart

Posth

et al. 2017

Sci Rep

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“…The calculation of the diffusion coefficient from the elution time becomes more complex if the cross-flow changes during elution. A numerical solution was therefore developed for trapezoidal channel and was improved for cross-flow decaying with the time [170][171][172].…”

Section: Separation Principlementioning

confidence: 99%

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

et al. 2018

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Abstract:Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross-linking occurs naturally during the storage and processing of milk and dairy products. Two size separation techniques were applied for characterisation of these reactions: gel electrophoresis and size exclusion chromatography. This review summarises their separation principles and discusses the outcome of studies on cross-linked casein from the last~20 years. Both methods, however, show limitations concerning separation range and are applied mainly under denaturing and reducing conditions. In contrast, field flow fractionation has a broad separation range and can be easily applied under native conditions. Although this method has become a powerful tool in polymer and nanoparticle analysis and was used in few studies on casein micelles, it has not yet been applied to investigate cross-linked casein. Finally, the principles and requirements for absolute molar mass determination are reviewed, which will be of increased interest in the future since suitable calibration substances for casein polymers are scarce.

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“…Bovine serum albumin, BSA (Sigma) solution (1 mg/mL), was used to validate the performance of the AF4 system and to determine the channel height [31]. According to a procedure described elsewhere [32], the thickness was determined to be 238 and 321 μm for channels with Millipore and MicrodynNadir regenerated cellulose membranes, respectively.…”

Section: Af4 Analysis Equipmentmentioning

confidence: 99%

Development and evaluation of methods for starch dissolution using asymmetrical flow field-flow fractionation. Part II: Dissolution of amylose

2015

Self Cite

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In this paper, we investigate whether dissolution in water under autoclaving conditions (140 °C, 20 min) or in dimethyl sulfoxide, DMSO (100 °C, 1 h), is preferable for characterization of amylose. Two types of amylose, potato and maize, were dissolved either in water using an autoclave or in DMSO. On the aqueous solutions obtained, the extent of molecular dissolution of the sample (referred to as the dissolution yield) was determined by enzymatic analysis as well as the molecular properties, such as molar mass and root-mean-square radius, obtained with asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and differential refractive index detection (AF4-MALS-dRI). The results showed that both dissolution methods are efficient at dissolving amylose. However, AF4-MALS-dRI analysis revealed substantial differences. Amylose aqueous solutions obtained by dissolution in DMSO were relatively stable over time, but the dissolution method in autoclave caused some degradation of the molecules, and their solutions display a high tendency to retrograde.

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Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part I. A theoretical approach

Cited by 61 publications

References 18 publications

Structural and magnetic properties of multi-core nanoparticles analysed using a generalised numerical inversion method

Structural and magnetic properties of multi-core nanoparticles analysed using a generalised numerical inversion method

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Development and evaluation of methods for starch dissolution using asymmetrical flow field-flow fractionation. Part II: Dissolution of amylose

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