Hydrodynamic Propulsion of Liposomes Electrostatically Attracted to a Lipid Membrane Reveals Size-Dependent Conformational Changes

Tabaei, Seyed R.; Gillissen, J. J. J.; Block, Stephan; Höök, Fredrik; Cho, Nam‐Joon

doi:10.1021/acsnano.6b04572

Cited by 14 publications

(30 citation statements)

References 77 publications

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“…Similar conclusions were previously derived from diffusivity and drift velocity measurements of membrane-adhering liposomes in sheared glucose solutions. 27 Based on geometrical considerations (supporting section S10), the observation of a constant liposome height suggests, that the reduced liposome diffusivity (cf. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Measuring both the diffusivity and the drift velocity, allowed computing both the contact area and the height of the liposomes. 27 By adding 500 mM glucose, the contact area was observed to increase, without appreciable changes in the liposome height. 27 This, together with the analysis in supporting section S10, suggests that glucose induces adhesion forces, which enhance the contact area, without changing the liposome height.…”

Section: Liposome Deformation On the Quartz Crystal Microbalancementioning

confidence: 97%

“…To further confirm the Brownian character of the liposome diffusivity, the inset of It is re-emphasized, that we interpret the liposome diffusivity data, by assuming, that the Brownian motion of the liposome is electrostatically slaved to that of a disk-shaped cluster of lipids in the underlying membrane. [25][26][27] The size of the cluster is referred to as the contact area, which is defined as the membrane region, within one Debye length to the liposome; see Fig. 1b…”

Section: Liposome Diffusivity On Supported Membranesmentioning

confidence: 99%

“…12,33 In previous work, we furthermore found that adding glucose (nonionic osmolyte) reduces the diffusivity, and we elucidated the governing mechanism, by applying a shear flow over the membrane. 27 By measuring both the diffusivity as well as the shear-induced drift velocity of the liposomes, we computed the hydrodynamic height of the liposomes, showing negligible change after adding glucose. Assuming that the membrane viscosity is unaltered, this observation would suggest, that glucose extends the intermembrane contact area via adhesion forces, rather than via osmotic forces.…”

Section: Introductionmentioning

confidence: 99%

“…In Ref. [27] it was hypothesized, that the responsible adhesion force is a "depletion force". Owing to its large size (~1.5 nm), it is conceivable that glucose molecules are depleted from the intermembrane hydration layer (~1 nm), 34 causing an adhesive force, [35][36] as illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Glucose on the Mobility of Membrane-Adhering Liposomes

et al. 2017

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Enclosed lipid bilayer structures, referred to as liposomes or lipid vesicles, have a wide range of biological functions, such as cellular signaling and membrane trafficking. The efficiency of cellular uptake of liposomes, a key step in many of these functions, is strongly dependent on the contact area between a liposome and a cell membrane, which is governed by the adhesion force w, the membrane bending energy κ, and the osmotic pressure Δp. Herein, we investigate the relationship between these forces and the physicochemical properties of the solvent, namely, the presence of glucose (a nonionic osmolyte). Using fluorescence microscopy, we measure the diffusivity D of small (∼50 nm radius), fluorescently labeled liposomes adhering to a supported lipid bilayer or to the freestanding membrane of a giant (∼10 μm radius) liposome. It is observed that glucose in solution reduces D on the supported membrane, while having negligible effect on D on the freestanding membrane. Using well-known hydrodynamic theory for the diffusivity of membrane inclusions, these observations suggest that glucose enhances the contact area between the small liposomes and the underlying membrane, while not affecting the viscosity of the underlying membrane. In addition, quartz crystal microbalance experiments showed no significant change in the hydrodynamic height of the adsorbed liposomes, upon adding glucose. This observation suggests that instead of osmotic deflation, glucose enhances the contact area via adhesion forces, presumably due to the depletion of the glucose molecules from the intermembrane hydration layer.

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Section: Resultsmentioning

confidence: 99%

Section: Liposome Deformation On the Quartz Crystal Microbalancementioning

confidence: 97%

Section: Liposome Diffusivity On Supported Membranesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Glucose on the Mobility of Membrane-Adhering Liposomes

et al. 2017

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Recruitment of Receptors and Ligands in a Weakly Multivalent System with Omnipresent Signatures of Superselective Binding

Allegri

Huskens

2023

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For instance, virus internalization by cells occurs in specialized regions where the recruitment of receptors takes place. [11] Typically, influenza A viruses infect their host by entering into cells via receptor-mediated endocytosis, [12,13] during which the distribution of corresponding receptors in the cell membrane is rearranged and co-localized, as a direct result of receptor clustering induced by weak and multiple ligand-receptor interactions. Thus, gaining understanding of the mechanism of receptor clustering induced by weakly multivalent interactions is essential in order to understand its effect in virus infection at the molecular level, and also to allow its application as a design rule in artificial self-assembling systems.In most natural biological systems, multivalent interactions are composed of many, but individually weakly binding, interaction pairs, which contributes to the dynamic character of the interactions. [14][15][16][17][18] We can relate this behavior to the fact that the overall affinity (K ov ) can be orders of magnitude higher than the individual, monovalent affinity (K i ). More precisely, each additional interaction pair contributes to the overall affinity by only a small enhancement factor, [1,19] which indicates at the same time that each interaction pair is dynamically equilibrating between its bound and unbound states, the frequency of which is dictated by the intrinsic dissociation rate constant. In contrast, as an opposing example of a strongly binding system, the individual affinity of biotin-streptavidin (SAv) is very high, [20,21] and as a result, assemblies based on multiple biotin-SAv interactions are viewed to be practically irreversible. [22][23][24] As an example of the dynamic nature of weak interactions, it has been reported that the low-density lipoprotein (LDL) receptors in the cell membrane will return to the cell membrane surface after the receptor-mediated endocytosis of a virus particle, where they again cluster in coated pits, [25,26] indicating that the LDL receptors still keep their mobility and are recycled in the recruitment process. This recycling property accounts for the fact that the receptors during clustering are still dynamic at their binding sites, allowing switching between the diffusive and the clustering states.To provide quantitative insight into these molecular processes, it is necessary to build synthetic model systems that Recruitment of receptors at membrane interfaces is essential in biological recognition and uptake processes. The interactions that induce recruitment are typically weak at the level of individual interaction pairs, but are strong and selective at the level of recruited ensembles. Here, a model system is demonstrated, based on the supported lipid bilayer (SLB) that mimics the recruitment process induced by weakly multivalent interactions. The weak (mm range) histidine-nickel-nitrilotriacetate (His 2 -NiNTA) pair is employed owing to its ease of implementation in both synthetic and biological systems. The recruitment of rece...

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