Hydrodynamic properties of bovine serum albumin monomer and dimer

Squire, Phil G.; Moser, P.; O’Konski, Chester T.

doi:10.1021/bi00852a018

Cited by 284 publications

(126 citation statements)

References 35 publications

(29 reference statements)

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“…They appear in an ellipsoidal shape with a major axis of 14 nm and a minor axis of 4 nm [52]. Protein adsorption to PAA brushes on STFs On flat samples, BSA has been shown to adsorb on PAA Guiselin brushes both under electrostatic attractive and moderately repulsive conditions [14].…”

Section: Protein Adsorption On Brushes On Stfsmentioning

confidence: 99%

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

et al. 2014

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A combined setup of quartz crystal microbalance and generalized ellipsometry can be used to comprehensively investigate complex functional coatings comprising stimuli-responsive polymer brushes and 3D nanostructures in a dynamic, noninvasive in situ measurement. While the quartz crystal microbalance detects the overall change in areal mass, for instance, during a swelling or adsorption process, the generalized ellipsometry data can be evaluated in terms of a layered model to distinguish between processes occurring within the intercolumnar space or on top of the anisotropic nanocolumns. Silicon films with anisotropic nanocolumnar morphology were prepared by the glancing angle deposition technique and further functionalized by grafting of poly-(acrylic acid) or poly-(N-isopropylacrylamide) chains. Investigations of the thermoresponsive swelling of the poly-(N-isopropylacrylamide) brush on the Si nanocolumns proved the successful preparation of a stimuli-responsive coating. Furthermore, the potential of these novel coatings in the field of biotechnology was explored by investigation of the adsorption of the model protein bovine serum albumin. Adsorption, retention, and desorption triggered by a change in the pH value is observed using poly-(acrylic acid) functionalized nanostructures, although generalized ellipsometry data revealed that this process occurs only on top of the nanostructures. Poly-(N-isopropylacrylamide) is found to render the nanostructures non-fouling properties.

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Section: Protein Adsorption On Brushes On Stfsmentioning

confidence: 99%

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

et al. 2014

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“…As mentioned in the literature, the drug release of proteins from a hydrogel is mainly based on passive diffusion through the hydrogel network [30,31], the rate being dependent upon the pore size and the hydrogel network homogeneity. To achieve prolonged release, the pore sizes of the network should be smaller than the hydrodynamic radius of the molecule of interest, i.e., 14 × 4 × 4 nm=224 nm 3 for BSA [32] and 0.38 nm 3 for lidocaine (estimated from its aqueous density and molar volume [33]). However, hydrogels often have a pore size that is too large, leading to an initial burst effect [34,35].…”

Section: Discussionmentioning

confidence: 99%

Influence of the Macro- and/or Microstructure of Cross-Linked Hyaluronic Acid Hydrogels on the Release of Two Model Drugs

Mondon¹,

Dadras²

2016

J Glycobiol

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Injectable hyaluronic acid (HA) hydrogels, crosslinked with 1,4-butanediol diglycidyl ether (BDDE), are widely used in aesthetic medicine. Due to their high clinical tolerance, HA hydrogels are thought to be applicable as injectable drug delivery systems. Here, HA matrix structures of BDDE-crosslinked HA hydrogels were analysed, and the effects of the structures on the release of two model drugs were assessed. Seven crosslinked HA hydrogels were observed by optical microscopy and cryo scanning electron microscopy (cryo-SEM). We observed three specific matrix macrostructures under optical microscopy: two had a "spider web"-like structure, three had a particulate structure, and two had an intermediate structure. These differences were less evident under cryo-SEM, where all hydrogels exhibited fibrous microstructures of different homogeneity levels, with pore sizes between 0.5 and 18 μm. Three cross-linked HA hydrogels with different macrostructures were loaded with bovine serum albumin (BSA) and lidocaine to assess their capacities to release drug over 4 days. No differences in drug release were observed between gels, and BSA was released for up to 4 days, which was four times longer than lidocaine. Thus, BDDEcrosslinked HA hydrogels could be applied as an injectable drug delivery system, particularly for the delivery of high-molecular-weight molecules.

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“…12,13 Other models for BSA include spherical subunit models which comprise one large sphere and two or three identical smaller spheres aligned in a linear chain. 13,14 As an example, we only consider the subunit model that comprises three-connected spheres ( Fig. 1(c)), noting that the mathematical formulation presented here can be easily extended to other spherical subunit models.…”

Section: Introductionmentioning

confidence: 99%

“…The dimer of BSA is also studied through the subunit model of threeconnected spheres. 13 In this paper, following ref. 12-14 we examine typical models of BSA, namely the prolate ellipsoid and the three-connected spheres.…”

Section: Introductionmentioning

confidence: 99%

Modelling bovine serum albumin inside carbon nanotubes

2013

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Bovine serum albumin is commonly used in many biochemical applications due to its stability, lack of interference within biochemical reactions and low production cost. Here, we model the interaction of bovine serum albumin inside a carbon nanotube. The carbon nanotube is chosen as an example to demonstrate its potential use in targeted drug and protein delivery and as an enzyme immobilizing material. We consider three possible structures as models for bovine serum albumin which are cylinder, prolate ellipsoid and threeconnected spheres. Using the Lennard-Jones potential together with a continuum approach we obtain explicitly analytical expressions for the interaction energies of each configuration inside a carbon nanotube. These expressions are employed to determine the critical size of a nanotube which maximises the interaction with each model structure of the bovine serum albumin. Knowledge of the critical size is important and may be crucial for the design of a nanotube for maximum loading of the proteins and drug molecules. Bovine serum albumin is commonly used in many biochemical applications due to its stability, lack of interference within biochemical reactions and low production cost. Here, we model the interaction of bovine serum albumin inside a carbon nanotube. The carbon nanotube is chosen as an example to demonstrate its potential use in targeted drug and protein delivery and as an enzyme immobilizing material. We consider three possible structures as models for bovine serum albumin which are cylinder, prolate ellipsoid and three-connected spheres. Using the Lennard-Jones potential together with a continuum approach we obtain explicitly analytical expressions for the interaction energies of each configuration inside a carbon nanotube. These expressions are employed to determine the critical size of a nanotube which maximises the interaction with each model structure of the bovine serum albumin. Knowledge of the critical size is important and may be crucial for the design of a nanotube for maximum loading of the proteins and drug molecules.

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Hydrodynamic properties of bovine serum albumin monomer and dimer

Cited by 284 publications

References 35 publications

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

Influence of the Macro- and/or Microstructure of Cross-Linked Hyaluronic Acid Hydrogels on the Release of Two Model Drugs

Modelling bovine serum albumin inside carbon nanotubes

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