Hydrodynamic focusing investigation in a micro-flow cytometer

Yang, An-Shik; Hsieh, Wen‐Hsin

doi:10.1007/s10544-006-9003-9

Cited by 26 publications

(21 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microfluidic methods for hydrodynamic focusing have been reported before Nieuwenhuis et al (2004); Simonnet and Groisman (2006); Chang et al (2007); Hairer and Vellekoop (2007); Yang and Hsieh (2007); Rodriguez-Trujillo et al (2008); Scott et al (2008); Watkins et al (2009), but none have used passive pumping. As shown in Figure 2, the relative radii of droplets on the focusing and sample inlets affects the width of the outer focusing streams and the inner sample stream.…”

Section: Resultsmentioning

confidence: 99%

A microfluidic passive pumping Coulter counter

McPherson

Walker

2010

Microfluid Nanofluid

View full text Add to dashboard Cite

A microfluidic device using on-chip passive pumping was characterized for use as a particle counter. Flow occurred due to a Young-Laplace pressure gradient between two 1.2 mm diameter inlets and a 4 mm diameter reservoir when 0.5μ L fluid droplets were applied to the inlets using a micropipette. Polystyrene particles (10μm diameter) were enumerated using the resistive pulse technique. Particle counts using passive pumping were within 13% of counts from a device using syringe pumping. All pumping methods produced particle counts that were within 16% of those obtained with a hemocytometer. The effect of intermediate wash steps on particle counts within the passive pumping device was determined. Zero, one, or two wash droplets were loaded after the first of two sample droplets. No statistical difference was detected in the mean particle counts among the loading patterns (p > 0.05). Hydrodynamic focusing using passive pumping was also demonstrated.

show abstract

Section: Resultsmentioning

confidence: 99%

A microfluidic passive pumping Coulter counter

McPherson

Walker

2010

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…The basic idea is derived from conventional flow cytometry (Lee et al 2001a;Yang and Hsieh 2007;Chung et al 2003). Flow cytometry in general (Ghosh et al 2008;Srisa-Art et al 2009), and hydrodynamic focusing methods in particular, are efficient techniques to divide, count, or even sort microparticles or different cell subpopulations (Liu et al 2009;Chen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Time correlated fluorescence characterization of an asymmetrically focused flow in a microfluidic device

Carlotto

Fortunati

Ferrante

et al. 2010

Microfluid Nanofluid

View full text Add to dashboard Cite

In this study, we explore and model the behavior of a prototype microfluidic device which employs two non-mixing fluids (sheath and inlet fluids) displaying an asymmetric focused flow, in the presence of a fluorescent dye. Fluorescence correlation spectroscopy is employed, allowing the precise measure of flow speeds across the channels and of the concentration profile of the central focused flux along the flow direction. The system is modeled via a standard Navier-Stokes finite-element approach, coupled to convection-diffusion equations for the solute. Simulations reproduce accurately the shape, the position, and the width of the velocity and concentration profiles along the central channel and across the transversal and vertical sections of the microfluidic device. The observed asymmetric flow with respect to the center of the channel is reproduced numerically with an error in the position determination smaller than 1%

show abstract

“…The ability to achieve well-ordered flows with uniform particle velocity and passage time is essential for accurate detection and characterization in such devices. The most common method of directing the particles through the channel involves the hydrodynamic focusing of the particle-bearing stream with secondary sheath flows 23,24. Recent advances have led to high-throughput and high-resolution cytometers capable of three-dimensional hydrodynamic focusing 25,26…”

Section: Introductionmentioning

confidence: 99%

High-throughput flow alignment of barcoded hydrogel microparticles

2009

View full text Add to dashboard Cite

Suspension (particle-based) arrays offer several advantages over conventional planar arrays in the detection and quantification of biomolecules, including the use of smaller sample volumes, more favorable probe-target binding kinetics, and rapid probe-set modification. We present a microfluidic system for the rapid alignment of multifunctional hydrogel microparticles designed to bear one or several biomolecule probe regions, as well as a graphical code to identify the embedded probes. Using high-speed imaging, we have developed and optimized a flow-through system that (1) allows for a high particle throughput, (2) ensures proper particle alignment for decoding and target quantification, and (3) can be reliably operated continuously without clogging. A tapered channel flanked by side focusing streams is used to orient the flexible, tablet-shaped particles into a wellordered flow in the center of the channel. The effects of channel geometry, particle geometry, particle composition, particle loading density, and barcode design are explored to determine the best combination for eventual use in biological assays. Particles in the optimized system move at velocities of ~50 cm s −1 and with throughputs of ~40 particles s −1 . Simple physical models and CFD simulations have been used to investigate flow behavior in the device.

show abstract

Hydrodynamic focusing investigation in a micro-flow cytometer

Cited by 26 publications

References 23 publications

A microfluidic passive pumping Coulter counter

A microfluidic passive pumping Coulter counter

Time correlated fluorescence characterization of an asymmetrically focused flow in a microfluidic device

High-throughput flow alignment of barcoded hydrogel microparticles

Contact Info

Product

Resources

About