Hydrodynamic chromatography of polymers in packed columns

In Vitro Blood Flow Behaviour in Microchannels with Simple and Complex Geometries

“…22. The authors (Couto et al, 2011) suggested that this behaviour can be explained by the theory that supports the hydrodynamic fractionation (Tijssen et al, 1986;Stegeman et al, 1993;Dias, 2007Dias, , 2008 and by the formation of a cell-free layer adjacent to the walls of the capillaries. …”

Hydrodynamic Chromatography of Silica Colloids on Small Spherical Nonporous Silica Particles

“…[2][3][4] Since its introduction, HDC has been applied to size characterization of synthetic polymers or particles, [4][5][6][7] biopolymers, 8,9 and lipid nanocapsules 10 as an attractive alternative to the classical separation methods such as size exclusion chromatography (SEC) or field-flow fractionation (FFF). HDC separations have been so far demonstrated on packed beds, 1,[3][4][5][6][7][8][9][10] open-tubular capillaries 2,[11][12][13][14][15] and microchips. [16][17][18][19] Packed-bed HDC is more versatile in terms of resolution and ease in handling than open-tubular and microchip HDC.…”

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

“…The modelling of transport of flexible polymers is much more complex than that of rigid spherical particles since the size and shape of the polymer are not well defined, being difficult to predict the distance (effective radius) to which the mass centre of the polymer can approach the wall of the capillary [7].…”

“…Stegeman et al (1993) [7] used PCHDC columns packed with non-porous glass spheres with diameter 1.5 μm and separated by HDC different Mw flexible polymers (polystyrene, polyisoprene, polyisobutadiene). Non-porous glass spheres with diameter 1 μm were used by Venema et al [8] in the separation of different Mw polysterenes and poly(methyl methacrylate).…”

“…Meanwhile, it was found application for HDC in a broad range of separations such as polymer latexes [2,3], rigid and flexible polymers [4][5][6][7][8][9][10][11][12][13], silica particles [14], zeolites [15], viruses [5], bacteria and yeasts [16], proteins [17], plasmid DNAs [17,18], starch [19,20], gold colloids [21], liposomes [21] and nanocapsules [22], these separations being performed using columns packed with nonporous particles (packed-column hydrodynamic chromatography (PCHDC)) or in open capillaries (capillary hydrodynamic chromatography (CHDC)).…”