Hydrodynamic chromatography of macromolecules on small spherical non-porous silica particles

Hydrodynamic Chromatography of Silica Colloids on Small Spherical Nonporous Silica Particles

“…[2][3][4] Since its introduction, HDC has been applied to size characterization of synthetic polymers or particles, [4][5][6][7] biopolymers, 8,9 and lipid nanocapsules 10 as an attractive alternative to the classical separation methods such as size exclusion chromatography (SEC) or field-flow fractionation (FFF). HDC separations have been so far demonstrated on packed beds, 1,[3][4][5][6][7][8][9][10] open-tubular capillaries 2,[11][12][13][14][15] and microchips. [16][17][18][19] Packed-bed HDC is more versatile in terms of resolution and ease in handling than open-tubular and microchip HDC.…”

“…Commercial sols contain 15 to 40% (w/v) SiO2 as spherical particles with diameters ranging from 4 to 200 nm. 20 Silica colloids and other inorganic colloids have been characterized by FFF, 21 SEC, 22,23 HDC, 4 and capillary-zone electrophoresis. 24,25 The advantages and limitations of these separation methods for characterizing the size of silica colloids and other inorganic colloids have been discussed.…”

Size Fractionation by Slalom Chromatography and Hydrodynamic Chromatography

“…In works [7,8,11,14] where PCHDC was used, it was assumed that the interstitial channels could be represented by a bundle of cylindrical capillaries with average radius, R, given by (d p / 3)( / (1 )) , d p being the particle diameter and the packing porosity. Since is given by ( /2) g R and R g can be calculated resorting to the R g -Mw relationships (available in the literature or determined experimentally), Eq.…”

“…Meanwhile, it was found application for HDC in a broad range of separations such as polymer latexes [2,3], rigid and flexible polymers [4][5][6][7][8][9][10][11][12][13], silica particles [14], zeolites [15], viruses [5], bacteria and yeasts [16], proteins [17], plasmid DNAs [17,18], starch [19,20], gold colloids [21], liposomes [21] and nanocapsules [22], these separations being performed using columns packed with nonporous particles (packed-column hydrodynamic chromatography (PCHDC)) or in open capillaries (capillary hydrodynamic chromatography (CHDC)).…”

Two‐dimensional chromatography as a tool for studying band broadening in size‐exclusion chromatography

“…Manufacturers specify values for commercial standards, which are usually upper limits. Several research groups [9,10,18] have demonstrated that these specifications overestimate the true PDI values. Other authors [9,10,19] have claimed that the PDI obtained by SEC is also an upper limit, because it is significantly affected by band-broadening phenomena.…”