“…The true solvent velocity should be calculated from a solute which is completely excluded from the pores. However, hydrodynamic (HDC) effects will then affect the retention times of such solutes rendering an erroneous value [12]. The velocity used in the figures was therefore obtained using the retention times of toluene.…”
Section: Selection Of the Organic Solventmentioning
confidence: 99%
“…Under pressure, the ratio between the measured pore radius (190 Ik) and the interstitial channel radius (equal to the hydraulic radius (=2dp/9) [12]) within the packing material (-1.1 ]am) is about 1:58, resulting in a co of 0.0003 and thus can be neglected.…”
Section: Retention Behaviour Of Psmentioning
confidence: 99%
“…This behaviour can be explained by a hydrodynamic effect which can occur in the inter particle space depending on the flow profile [12,17,18]. Under pressure drive the flow profile in the inter particle space may be assumed parabolic.…”
SummaryElectrically driven size exclusion chromatography (ED-SEC) of polystyrenes in packed capillaries using dimethylformamide as solvent is demonstrated. The efficiency and retention behaviour of polystyrenes under pressure and electro drive were investigated. Under pressure drive the plate height (H) increases steadily with increasing linear velocity (u) whereas under electro drive the H-u curves largely coincide and are very fiat. At higher velocities the plate heights are about 50 % smaller with electro drive than with pressure drive. Calculations show that with increasing ionic strength, the flow through the particles may increase causing a clear diminishing of the elution window.
“…The true solvent velocity should be calculated from a solute which is completely excluded from the pores. However, hydrodynamic (HDC) effects will then affect the retention times of such solutes rendering an erroneous value [12]. The velocity used in the figures was therefore obtained using the retention times of toluene.…”
Section: Selection Of the Organic Solventmentioning
confidence: 99%
“…Under pressure, the ratio between the measured pore radius (190 Ik) and the interstitial channel radius (equal to the hydraulic radius (=2dp/9) [12]) within the packing material (-1.1 ]am) is about 1:58, resulting in a co of 0.0003 and thus can be neglected.…”
Section: Retention Behaviour Of Psmentioning
confidence: 99%
“…This behaviour can be explained by a hydrodynamic effect which can occur in the inter particle space depending on the flow profile [12,17,18]. Under pressure drive the flow profile in the inter particle space may be assumed parabolic.…”
SummaryElectrically driven size exclusion chromatography (ED-SEC) of polystyrenes in packed capillaries using dimethylformamide as solvent is demonstrated. The efficiency and retention behaviour of polystyrenes under pressure and electro drive were investigated. Under pressure drive the plate height (H) increases steadily with increasing linear velocity (u) whereas under electro drive the H-u curves largely coincide and are very fiat. At higher velocities the plate heights are about 50 % smaller with electro drive than with pressure drive. Calculations show that with increasing ionic strength, the flow through the particles may increase causing a clear diminishing of the elution window.
“…This equation was derived using Eq. (13) by assuming that when the solute diameter reaches 35% of the pore diameter, it is unable to penetrate the pore [28]. In this work, the MW of upper exclusion limit for the Bio-Rad P60 gel (Bio-Rad Laboratories, Hercules, CA, USA) was set to 67,000 which is the MW of BSA.…”
“…All the aforementioned fractions were used later as starting values in the fitting procedure of the experimental points on the calibration curve by a theoretical function presented in [25]:…”
Section: Evaluation Of Monolith Porositymentioning
Monolithic capillary columns based on pentaerythritol triacrylate and pentaerythritol tetraacrylate were synthesized using different compositions of polymerization mixtures and different polymerization conditions. The impact of porogen type and porogen/monomer ratio on the porosity of synthesized monoliths was investigated. Porogen type appears to be the main factor influencing the separating properties of the monolithic sorbent. Using optimal polymerization conditions (porogen type, porogen/monomer ratio, reaction temperature, time etc.) monoliths with a porous structure optimized for polymer separations can be obtained. The monolithic capillary columns containing porous sorbents with optimized porosity are capable of separating 10 to 12 polystyrene standards in one chromatographic run utilizing both size exclusion chromatography and hydrodynamic chromatography separation mechanisms.
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