Hydrodynamic and oxygen mass transfer studies in a three-phase (air–water–ionic liquid) stirred tank bioreactor

Torres-Martínez, D.; Melgarejo-Torres, Rodrigo; Gutiérrez-Rojas, M.; Aguilera-Vázquez, Luciano; Micheletti, Martina; Lye, Gary J.; Huerta‐Ochoa, Sergio

doi:10.1016/j.bej.2009.03.014

Cited by 36 publications

(13 citation statements)

References 19 publications

(29 reference statements)

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“…The extractive fermentation technology is based on the addition of a second liquid phase (immiscible in water) for the in situ removal of toxic metabolic products in order to avoid cell inhibition (Bruce and Daugulis, 1991;van den Berg et al, 2008). Additionally, if the process involves the biotransformation of toxic substrates, the immiscible liquid phase can act as a substrate reservoir.…”

Section: Whole-cell Processesmentioning

confidence: 99%

“…Torres-Martinez et al (2009) [NTF 2 ], determined the effect of aeration, agitation and IL percentage on both the overall oxygen mass transfer coefficient (K L a) and the IL droplet diameter. They found that for all the ILs tested, the K L a values ranged from 80 to 200 h À1 at an agitation rate of 700 min À1 , aeration of 1 vvm, and 12.5% (v/v) of IL.…”

Section: Mass Transfer In Gas/ionic-liquid/water Dispersionsmentioning

confidence: 99%

See 1 more Smart Citation

Ionic liquids: Applications and future trends in bioreactor technology

Quijano

Couvert

Amrane

2010

Bioresource Technology

182

108

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Section: Whole-cell Processesmentioning

confidence: 99%

Section: Mass Transfer In Gas/ionic-liquid/water Dispersionsmentioning

confidence: 99%

Ionic liquids: Applications and future trends in bioreactor technology

Quijano

Couvert

Amrane

2010

Bioresource Technology

182

108

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“…Chùm tia Laser đơn sắc (bước sóng 782 nm) từ nguồn được dẫn đến hệ thấu kính (3) bằng sợi quang học đơn (single-fiber) với tiết diện 4 µm (5) và bộ chia quang (6) đến bộ định hướng (4). Sau khi đi vào hệ thấu kính động (tốc độ góc 2 m/s), chùm hội tụ (2) tạo ra điểm hội tụ tại vị trí (7) trước cửa sổ thạch anh (1) [7,8]. Christian Hahn đã áp dụng thành công kỹ thuật này để theo dõi quá trình lên men sữa [9].…”

Section: Phương Pháp Phản Xạ Tia Laser ứNg Dụng Trong Xác địNh Phân Bunclassified

Advanced methods for in situ chemical and bio processes analysis based on the Laser back-reflectance measurement technology

Lê¹,

Schwartz²

2016

Sci. Tech. Dev. J.

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Determination of the particle size distribution (PSD) is one of the most important tasks to monitor and control for most of chemical and bio-processes. In continuous movement of particles under effects of the carrying fluid, PSD determination is a technically challenging. This article summarizes some researches in which Laser back reflectance measurement method was used for in-situ and in real time PSD analysis. Two processes are selected to introduce including: crystallization of the amino acid L-arginine and biological processes.

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“…shake flasks), traditionally equipped with different types of sterile closures, are very useful tools in biotechnology. This case was discussed by Fernandes and Cabral [1], Vojinović et al [2], Baganz and coworkers [3,4], Maier and co‐workers [5,6], Wurm and co‐workers [7–9], Lye and co‐workers [10,11] and García Camacho and co‐workers [12,13]. The gas transfer coefficient of the sterile closures ( k plug ) plays an important role in aeration of shaken bioreactors.…”

Section: Introductionmentioning

confidence: 99%

Development of an unsteady‐state model for a biological system in miniaturized bioreactors

Amoabediny

Ziaie-Shirkolaee

Büchs

2009

Biotech and App Biochem

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In the present study, the special shake flasks, so-called ventilation flasks, are equipped with oxygen sensors and then an unsteady-state gas transfer model for shake flasks was developed and experimentally investigated for a wide range of gas transfer resistances (kplug). For the validation of our unsteady-state model to simulate the gas transfer in a biological system in the ventilation flasks, a strain of Corenobacterium glutamicum DM1730 was used as a model organism. For further easy processing, the resulting total mass-transfer resistance (kplug) is described as a function of the mass flow through the sterile plug (OTRplug) by an empirical equation. This equation is introduced into a simulation model that calculates the gas partial pressures in the headspace of the flask. Additionally, the gas transfer rates through the sterile closure and gas/liquid interface inside the flask are provided. This unsteady-state model would be a very useful method for scaling up from a shake flask to a fermentor; comparing the results of the gas concentration in the gas phase, there is good agreement between the introduced unsteady-state model and experimental results for the biological system.

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Hydrodynamic and oxygen mass transfer studies in a three-phase (air–water–ionic liquid) stirred tank bioreactor

Cited by 36 publications

References 19 publications

Ionic liquids: Applications and future trends in bioreactor technology

Ionic liquids: Applications and future trends in bioreactor technology

Advanced methods for in situ chemical and bio processes analysis based on the Laser back-reflectance measurement technology

Development of an unsteady‐state model for a biological system in miniaturized bioreactors

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