Hydrocortisone Long-term Treatment Effect on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stromal/Stem Cells

Salimiyan, Samira; Mohammadi, Mobin; Aliakbari, Sara; Kazemi, Romina; Amini, Abbas; Rahmani, Mohammadreza

doi:10.1089/jir.2021.0120

Cited by 2 publications

(1 citation statement)

References 46 publications

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“…Corticosterone, unlike cortisol in humans, is the main glucocorticoid hormone in rodents like rats. It is involved in immune and stress responses amongst other functions [22,23].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

2022

JCEI

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Background: Bio-Clean II has been previously shown to fight viral infection, boost immunity, and possesses anti-inflammatory properties by regulating the serum level of inflammatory cytokines, as well as T-Helper 4 and Cytotoxic T-Lymphocytes in rats exposed to purified bacterial lipopolysaccharide (LPS). Aim: The aim of this study is to assess the effects of Bio-Clean II on the C-reactive protein (CRP), corticosterone (CORT) and anti-phospholipid antibodies (aPLs) levels in rats exposed to bacterial lipopolysaccharide (LPS) using animal model. Materials and Methods: A total of 36 male Wistar rats weighing 150g±50g (mean±SD) were purchased and randomly assigned to six (6) groups of 6 rats each. Group 1, 2, 3, 5 and 6 were induced with a single dose of 5mg/Kg of purified LPS® (E.coli 0127:B8, Sigma-Aldrich, St. Louis, USA), administered through intraperitoneal route using 1ml sterile needle and syringe, except for group 4 which served as the zero control (given water and food only throughout the experiment). Group 1 served as the inflammation control. Group 2 which served as the positive control received 50 mg diclofenac/kg [bid] and 500 mg ciprofloxacin/kg [bid] (positive control) in place of the Bio-Clean. Group 3 which served as the negative control received sterile phosphate buffer saline (PBS). While rats in group 5 and group 6 were treated orally with the herbal remedy “Bio-Clean II” for 7 days and 14 days, respectively. After which, the rats were killed and a cardiac blood specimen was taken from each rat and transferred to plain bottles to clot. Serum was obtained from the clotted blood by centrifugation. The serum levels of C-reactive protein, corticosterone and antiphospholipid antibodies were assayed using ELISA kits, supplied by Elabscience Biotechnology Inc, USA. Data generated were subjected to Statistical Package for Social Scientists-Version 20 (SPSS-20). Results: The outcome of this results show that the serum level of C-reactive protein of the 7 days (1.05±0.06ng/ml) and 14 days (0.93±0.05ng/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.002 and p=0.000, respectively) when compared to the inflammation control group (1.70±0.07ng/ml). Similarly, the serum level of anti-phospholipid antibodies of the 7 days (6.40±0.67 Uml) and 14 days (4.27±0.66 U/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.02 and p=0.008, respectively) when compared to the inflammation control group (16.47±1.53 Uml). Meanwhile, the corticosterone level of the 7 days Bio-clean treated rats (9.40±1.30ng/mL) was found to be non-significantly lower (p=0.812) in comparison to the inflammation control (13.50±2.50ng/mL); while that of the 14 days Bio-clean II treated rats (6.80±1.00ng/mL) was significantly lower (p=0.026). Conclusion: The outcom

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“…Corticosterone, unlike cortisol in humans, is the main glucocorticoid hormone in rodents like rats. It is involved in immune and stress responses amongst other functions [22,23].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

2022

JCEI

View full text Add to dashboard Cite

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Repeated intravenous doses of human umbilical cord-derived mesenchymal stromal cells for bronchopulmonary dysplasia: results of a phase 1 clinical trial with 2-year follow-up

Cerro Marín,

Ormazábal,

Gimeno-Navarro

et al. 2024

Cytotherapy

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Hydrocortisone Long-term Treatment Effect on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stromal/Stem Cells

Cited by 2 publications

References 46 publications

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

Repeated intravenous doses of human umbilical cord-derived mesenchymal stromal cells for bronchopulmonary dysplasia: results of a phase 1 clinical trial with 2-year follow-up

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