Hydrocortisone binding receptor in the rat pituitary GH3 cell line

Makover, Shraga; Wright, Rosemary; Telep, Elizabeth

doi:10.1016/0024-3205(76)90235-6

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…The cytosol as well as extracts prepared from crude nuclear fractions revealed specific binding activities for estradiol; the respective cytosol and nuclear dissociation constants were in agreement with earlier reports (14). The presence of a whole set of receptor proteins for different steroids, including progesterone, estradiol, hydrocortisone (15) and very likely androgens (not yet established), raises the question, whether these steroid receptor proteins are present in a single cell or if we are dealing with a mixture of clones, since GH3 cells are known to give rise to subclones of different characteristics (16). One argument for the simultaneous existence of different receptors in one cell is the fact that in GH3 cells progesterone receptor levels are under regulatory control of estradiol.…”

Section: Discussionmentioning

confidence: 99%

Progesterone and Estrogen Receptors in GH3 Cells

Roos¹,

Strittmatter²,

Fabbro³

et al. 1980

Horm Res

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GH3 cells are shown to contain cytosolic progesterone receptor in a mean concentration of 270 fmol/mg protein with a dissociation constant (K_D) for promegestone of 3 × 10^-9M (4°C). Estrogen receptor (K_D = 1.8 × 10^-10M) is demonstrated in cytosol (121 fmol/mg protein) as well as in 0.4 M KCl extracts (89 fmol/mg protein) of crude nuclear fractions. No progesterone receptor was detectable in the nuclear fraction. Both receptors are characterized by isoelectric focussing and by competition experiments. Addition of 10^-8M estradiol to the growth medium increases progesterone receptor levels up to five, suggesting that the progesterone receptor is under estrogenic control.

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Section: Discussionmentioning

confidence: 99%

Progesterone and Estrogen Receptors in GH3 Cells

Roos¹,

Strittmatter²,

Fabbro³

et al. 1980

Horm Res

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Glucocorticoid Receptors

Rousseau¹,

Baxter²

1979

Glucocorticoid Hormone Action

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Isolated ACTH deficiency with transitory GH deficiency

Hochberg

Hardoff

Atias

et al. 1985

J Endocrinol Invest

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A nine-year-old girl, who presented with a severe hypoglycemic coma, proved to have isolated ACTH deficiency, a finding previously reported in only two children. On the initial evaluation, before any treatment, GH did not respond to provocative stimuli. On replacement therapy with hydrocortisone, normal linear growth was observed. Repeated testing while on glucocorticoids replacement four years after the initial attack revealed normal GH response to stimulation test. It is suggested that cortisol deficiency was responsible for the severe hypoglycemic coma and subnormal GH response. A similar mechanism is speculated for the normal growth observed in some patients with apparent deficiency of anterior pituitary hormones, including GH. The possibility of permanent ACTH deficiency and transitory GH deficiency following hypophysitis is discussed.

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Hydrocortisone binding receptor in the rat pituitary GH3 cell line

Cited by 3 publications

References 8 publications

Progesterone and Estrogen Receptors in GH3 Cells

Progesterone and Estrogen Receptors in GH3 Cells

Glucocorticoid Receptors

Isolated ACTH deficiency with transitory GH deficiency

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