Hydrogeochemical characteristics, controlling factors, and recharge sources of the seismic observation wells in Shandong Province were investigated by analyzing cation and anion concentrations, hydrogen and oxygen isotopes in well water. A total of 17 water samples in seismic observation wells were collected on April 25-29, 2018. The results show that temperatures of seismic observation wells were in the range of 14.8 to 52.1°C, and the values of δD and δ 18 O ranged from -72.4‰ to -37.9‰ and from -9.4‰ to -4.3‰, respectively. Using C.A. ЩукаЛев’s classification method, the water samples of 17 seismic observation wells were classified into 7 types: Cl·SO 4 -Na·Ca, SO 4 -Na, Cl-Na, HCO 3 -Na·Ca, HCO 3 -Mg·Na·Ca, HCO 3 -Na and HCO 3 -Mg·Ca·Na. The results indicate that the hydrogeochemical characteristics of 17 seismic observation wells, with a certain spatial distribution pattern, are affected by several factors, such as the tectonic, topography, stratigraphy, hydrology and meteorology. The analyses of ratio coefficients, Schooller diagram, hydrogen and oxygen isotopes compositions, Giggenbach and Gibbs diagram suggest that the atmospheric precipitation is the main recharge source of 17 observation wells. The recharge sources of deep lateral runoff and sedimentation water, moreover, play a significant role in some seismic observation wells. Combined with the amount of precipitation, the distance from recharge areas, the closure degree of observation wells and the stage of water-rock reactions, the development directions of faulting and topography control the directions of groundwater recharge, runoff and discharge, which make hydrogeochemical characteristics represent complex spatial distribution rules.