Hydrocarbons and fatty acid methyl esters in bacterial biomass before and after physicochemical treatment

Botvinko, I. V.; Попова, О. В.; Stroeva, A. R.; Shuvalov, S. A.; Винокуров, В. А.

doi:10.1134/s0026261714020039

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and P. aeruginosa (Botvinko et al, 2014). Extracellular methyl palmitate, methyl stearate and methyl oleate were also found in methylotrophic bacteria as growth factors and adaptogens (Botvinko et al, 2014). The fact that the saturated normal fatty acids in this study are dominated by C 16 and C 18 homologues and that they occurred exclusively in the form of methyl esters seem to again imply a significant input from bacteria biomass during coalification.…”

Section: Accepted Manuscriptmentioning

confidence: 48%

“…Methyl esters of palmitic acid (C 16 ) and stearic acid (C 18 ) were also identified in extracts of bacterial biomass, for example, Arthrobacter sp. and P. aeruginosa (Botvinko et al, 2014). Extracellular methyl palmitate, methyl stearate and methyl oleate were also found in methylotrophic bacteria as growth factors and adaptogens (Botvinko et al, 2014).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

See 1 more Smart Citation

Characterisation of bioavailability of Surat Basin Walloon coals for biogenic methane production using environmental microbial consortia

Chen

Zheng

Hamilton

et al. 2017

International Journal of Coal Geology

View full text Add to dashboard Cite

The study sets out to characterize the bioavailability of six Surat Basin Walloon coals from different stratigraphic layers in a single borehole to environmental methanogenic consortia. Factors that control bioavailability have also been investigated on grounds of coal petrographic composition and the organic composition of solvent-extractable matter. Finely crushed coal core samples were inoculated with digested sludge culture from domestic wastewater treatment in serum bottles kept anoxically before incubation at mesophilic temperature over 30 days for biomethane production. Degradation of coal compounds was demonstrated via GC-MS characterization of methanol and dichloromethane (DCM) extracts, in combination with aqueous volatile fatty acids and alcohols and TOC (total organic carbon) analysis on fresh and microbially-digested coal samples. The resulting methane yields ranged from 14 to 33 μmol/g, with an average of 21 μmol/g (0.515 m 3 /tonne), comparable to those previously reported for subbituminous coals. Organic solvent-extractable materials that accounted for 3.8 to 12% of coal weight were generally dominated by aliphatic compounds, composed of mainly medium-long-chain n-alkanes, n-alcohols and esters. Aromatics were detected up to three fused rings, and are rich in dibenzofuran, alkyl benzene, diphenyls and alkyl PAH (polymeric aromatic hydrocarbon). The abundance of solvent-extractable matter was found to be positively associated with liptinite content, particularly suberinite, sporinite and liptodetrinite. Preservation of these compounds was thought to rely on vitrinite, such as telinite and collotelinite that are rich in micropores, serving as storage for the hydrocarbons. Environmental factors, such as microbe-carrying groundwater might compromise coal extractability by converting coal hydrocarbons to biogas. Bioavailability of coal was shown to be controlled by three factors: 1) Water solubility-Bioassay eliminated an average 98% of aqueous compounds (based on TOC), which were dominated by volatile fatty acids and alcohols, and to a lesser degree, medium-chain (primarily C 10 to C 20) n-alcohols, esters and aliphatic amine; 2) Solvent extractability-34.5% of solvent-extractable compounds were shown to be biodegraded (based on peak intensity in GC-MS), with methanol extracts being more bioavailable than DCM's; 3) Heterogeneous moieties, particularly aliphatic hydroxyl group, ester bond, ether bond and C-N bond in aliphatic amine-These functional groups present heteroatoms that can lower the activation energy of nearby bonds, making them vulnerable for microbial cleavage. Compound degradation in bioassays was shown to be clearly associated with methane yield, but only a small proportion degraded was converted to methane. Further improvement may be achieved via proper adaptation of the current microbial community.

show abstract

Section: Accepted Manuscriptmentioning

confidence: 48%

Section: Accepted Manuscriptmentioning

confidence: 99%

Characterisation of bioavailability of Surat Basin Walloon coals for biogenic methane production using environmental microbial consortia

Chen

Zheng

Hamilton

et al. 2017

International Journal of Coal Geology

View full text Add to dashboard Cite

show abstract

“…However, higher alkanes are dominated by n alkanes with an even number of carbon atoms С 22 , С 24 , С 30 , С 32 , or С 34 . It was found that both strains synthesize the unsaturated irregular isoprenane squalene (2,6,10,15,19,23 hexamethyltet racosa 2,6,10,14,18,22 hexaene) [4][5][6].…”

mentioning

confidence: 99%

Modeling of formation of petroleum biomarker hydrocarbons by thermolysis and thermocatalysis of bacterium biomass

et al. 2014

Self Cite

View full text Add to dashboard Cite

Thermolysis and thermocatalysis of the insoluble part of chemoorganoheterotrophic aerobic bac teria Arthrobacter sp. RV and Pseudomonas aeruginosa RM have been performed. The thermolyzates and thermocatalyzates of these bacteria contain the hydrocarbon biomarkers n alkanes, isoprenanes, steranes, and terpanes. Of n alkanes, the C 9 -C 35 hydrocarbons with unimodal distribution formed in the products of or C 9 -C 39 n alkanes with bimodal distribution are produced by thermolysis or thermocatalysis, respectively. n Alkanes with odd number of carbon atoms in the molecule prevail over the even counterparts (n C 9 , n C 11 , n C 15 , and n C 17 ) in the thermolysis products of both strains, whereas n alkanes with even number of carbon atoms (n C 16 , n C 18 , and n C 20 ) dominate in the thermocatalyzates. Isoprenanes of the C 13 -C 20 composi tion are generated. It is noteworthy that regular C 17 isoprenane has been found for the first time among iso prenanes. The cyclic biomarker hydrocarbon steranes and terpanes are simultaneously generated, with the distribution of C 27 -C 29 regular steranes resembles that in marine oils generated in argillaceous strata. At the same time, the adiantane to hopane ratio (H 29 /H 30 ) is characteristic of the organic matter generated in car bonate strata.Keywords: chemoorganoheterotrophic aerobic bacteria, formation of petroleum hydrocarbons by thermolysis and thermocatalysis, biomarker hydrocarbons

show abstract

Bioavailability of coal for microbially enhanced methane production

Chen¹

View full text Add to dashboard Cite

Hydrocarbons and fatty acid methyl esters in bacterial biomass before and after physicochemical treatment

Cited by 3 publications

References 14 publications

Characterisation of bioavailability of Surat Basin Walloon coals for biogenic methane production using environmental microbial consortia

Characterisation of bioavailability of Surat Basin Walloon coals for biogenic methane production using environmental microbial consortia

Modeling of formation of petroleum biomarker hydrocarbons by thermolysis and thermocatalysis of bacterium biomass

Bioavailability of coal for microbially enhanced methane production

Contact Info

Product

Resources

About