Hydrocarbon bioremediation on Arctic shorelines: Historic perspective and roadway to the future

Góngora, Esteban; Chen, Ya-Jou; Ellis, Madison; Okshevsky, Mira; Whyte, Lyle G.

doi:10.1016/j.envpol.2022.119247

Cited by 8 publications

(1 citation statement)

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“…The ability of microorganisms to degrade pollutants, including crude oil hydrocarbons (primarily n -alkanes and mono- and polyaromatic compounds), remains one of the most important topics of modern biotechnological research 8 , 9 . This ability makes it possible to use microorganisms in technologies for cleanup of the environment from crude-oil pollution 10 , 11 .…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas veronii strain 7–41 degrading medium-chain n-alkanes and polycyclic aromatic hydrocarbons

Mullaeva

Delegan

Streletskii

et al. 2022

Sci Rep

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Pollution of the environment by crude oil and oil products (represented by various types of compounds, mainly aliphatic, mono- and polyaromatic hydrocarbons) poses a global problem. The strain Pseudomonas veronii 7–41 can grow on medium-chain n-alkanes (C8–C12) and polycyclic aromatic hydrocarbons such as naphthalene. We performed a genetic analysis and physiological/biochemical characterization of strain 7–41 cultivated in a mineral medium with decane, naphthalene or a mixture of the hydrocarbons. The genes responsible for the degradation of alkanes and PAHs are on the IncP-7 conjugative plasmid and are organized into the alk and nah operons typical of pseudomonads. A natural plasmid carrying functional operons for the degradation of two different classes of hydrocarbons was first described. In monosubstrate systems, 28.4% and 68.8% of decane and naphthalene, respectively, were biodegraded by the late stationary growth phase. In a bisubstrate system, these parameters were 25.4% and 20.8% by the end of the exponential growth phase. Then the biodegradation stopped, and the bacterial culture started dying due to the accumulation of salicylate (naphthalene-degradation metabolite), which is toxic in high concentrations. The activity of the salicylate oxidation enzymes was below the detection limit. These results indicate that the presence of decane and a high concentration of salicylate lead to impairment of hydrocarbon degradation by the strain.

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