Hydro-Ethanolic Extract of Mentha pulegium Exhibit Anthelmintic and Antioxidant Proprieties In Vitro and In Vivo

Sebai, Essia; Serairi, Raja; Saratsi, Katerina; Abidi, Anouar; Sendi, Nadia; Darghouth, Mohamed Aziz; Wilson, Mark S.; Sotiraki, Smaragda; Akkari, Hafidh

doi:10.2478/s11686-020-00169-3

Cited by 18 publications

(6 citation statements)

References 57 publications

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“…Similar components were also found in the leaves and flowers of small wild plants growing in Saudi Arabia named Lantana camara , Anvillea garcinii , and Strychnos nux-vomica [ 8 , 9 , 10 ]. In vitro studies showed that mint extracts from the Tunisian genus exhibited important antioxidant, antiviral, antiallergenic, and antimicrobial activities [ 11 , 12 , 13 ]. In addition, previous studies have demonstrated the potent antitumor activities of the Mentha species [ 14 , 15 ] as an effective chemopreventive agent [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

et al. 2022

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Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

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Section: Introductionmentioning

confidence: 99%

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

et al. 2022

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“…1 F–H). The stability is due to the presence of metabolites in pennyroyal derivations, such as syringic acid, ferulic acid, isorhamnetin-3-O-glucoside, and kaempferol-3-O-rutinoside 20 . The results of the FT-IR spectrum of ZnO/Mlt-NC showed three classes of bands.…”

Section: Resultsmentioning

confidence: 99%

“…It contains some phenolic compounds, such as syringic acid, ferulic acid, and flavonoid compounds, including isorhamnetin-3-O-glucoside and kaempferol-3-O-rutinoside that can contribute to capping and stabilizing. Therefore, in the present study, for the first time, M. pulegium extract was used to synthesize ZnO/Mlt-NC 20 .…”

Section: Introductionmentioning

confidence: 99%

Biofabrication of ZnO/Malachite nanocomposite and its coating with chitosan to heal infectious wounds

Rajabloo

Farahpour

Saffarian

et al. 2022

Sci Rep

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Recently, nanocomposites produced from clays and metals coated with chitosan have shown wound healing activity. This study aimed to synthesize the zinc oxide/malachite nanocomposite (ZnO/Mlt-NC) and its coating form with chitosan (ZnO/Mlt/Chsn-NC). Physicochemical characterization of the produced nanocomposites was investigated. Biomedical effects of nanocomposites, such as in vivo and in vitro antibacterial activity, antioxidant properties, cytotoxicity, and modulation in the gene expressions of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β) and histopathological parameters, were also investigated. Expression intensities of basic fibroblast growth factor (bFGF) and tumor necrosis factor alpha (TNF-α) were also investigated by immunofluorescence staining. To investigate biomedical effects under in vivo conditions, infected wounds were induced and inoculated with Staphylococcus aureus (ATCC 25923), and Pseudomonas aeruginosa (ATCC 27853). The results indicated spherical ZnO nanoparticles on the surface of malachite and strong antibacterial activity and antioxidant properties. The ointments produced from the nanocomposites also exhibited wound healing activity. The administration of the ointments prepared from ZnO/Mlt, and ZnO/Mlt/Chsn NCs decreased the expressions of IL-1β, IL-6, and TNF-α, while it increased the expressions of IL-10, TGF-β and bFGF. In sum, the nanocomposites produced from ZnO, malachite, and chitosan had better biological activity than ZnO/Malachite nanocomposites. We suggest applying ZnO/Mlt/Chsn nanocomposites in the structure of ointments to treat infected wounds after future clinical studies.

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“…Therefore, it can be stated that both SB juices were endowed with high anthelmintic activity against larvae (L3), and only SBT also against eggs. Moreover, the SBT tested in this study (EC 50 = 519 µg/mL) was 24.7 and 3.8 times more potent in inhibiting GIN egg hatching, respectively, compared to ethanolic extracts from Mentha pulegium (EC 50 = 12,800 µg/mL) [ 50 ] and Artemisia campestris (EC 50 = 2000 µg/mL) [ 51 ], which were evaluated in previous studies.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anthelmintic Activity of Sea Buckthorn (Hippophae rhamnoides) Berry Juice against Gastrointestinal Nematodes of Small Ruminants

Maestrini

Forzato

Mancini

et al. 2022

Biology

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Gastrointestinal nematodes are one of the major threats in small ruminant breeding. Their control is difficult due to the development of anthelmintic resistance, and the search for new molecules endowed with anthelmintic activity (AH) is considered a priority. In this context, we evaluated the in vitro AH activity of two commercial sea buckthorn (Hippophae rhamnoides) berry juices, namely SBT and SBF. The in vitro evaluation was based on the egg-hatch test and larval exsheathment assay at different concentrations. Data were statistically analysed, and the EC50 was calculated. Chemical analyses were performed to evaluate the total polyphenol content of the juices and chemical profile of the most represented compounds. The role of the polyphenolic fraction in the anthelmintic activity of the juices was also assessed. At the highest concentrations, the activity of SBT was high in both tests and comparable to that observed in the thiabendazole-treated positive controls, while SBF showed a lower efficacy. Glycosylated isorhamnetin and quercetin were the most represented polyphenolic compounds in both juices. In conclusion, both H. rhamnoides berry juices tested in this study showed interesting anthelmintic properties in vitro.

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Hydro-Ethanolic Extract of Mentha pulegium Exhibit Anthelmintic and Antioxidant Proprieties In Vitro and In Vivo

Cited by 18 publications

References 57 publications

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Biofabrication of ZnO/Malachite nanocomposite and its coating with chitosan to heal infectious wounds

In Vitro Anthelmintic Activity of Sea Buckthorn (Hippophae rhamnoides) Berry Juice against Gastrointestinal Nematodes of Small Ruminants

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