Hydraulic flux–responsive hormone redistribution determines root branching

Mehra, Poonam; Pandey, Bharati; Melebari, Dalia; Banda, Jason; Leftley, Nicola; Couvreur, Valentin; Rowe, James; Anfang, Moran; Gernier, Hugues De; Morris, Emily; Sturrock, Craig J.; Mooney, Sacha J.; Swarup, Ranjan; Faulkner, Christine; Beeckman, Tom; Bhalerao, Rishikesh P.; Shani, Eilon; Jones, Alexander M.; Dodd, Ian C.; Sharp, Robert E.; Sadanandom, Ari; Bennett, Malcolm J.

doi:10.1126/science.add3771

Cited by 46 publications

(34 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…29,30 Recently, water and auxin fluxes in the root basal meristem through plasmodesmata were reported, establishing a symplastic pathway to deliver auxin from the epidermis to the pericycle. 31 The effect of auxin on plant growth is determined by auxin levels and distribution; therefore, a particular pattern of auxin distribution is required for root development. The distribution of endogenous auxin in LRFCs is altered by the inhibition of basipetal auxin transport, which blocks LR initiation.…”

Section: Introductionmentioning

confidence: 99%

ERF1 inhibits lateral root emergence by promoting local auxin accumulation with altered distribution and repressingARF7expression

Zhao

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

SummaryLateral roots (LRs) are crucial for plants to sense environmental signals in addition to water and nutrient absorption. Auxin is key for LR formation, but the underlying mechanisms are not fully understood. Here we report thatArabidopsisERF1 inhibits LR emergence by promoting local auxin accumulation with altered distribution and regulating auxin signaling. Loss ofERF1increases LR density compared with the wild type, whereasERF1overexpression causes the opposite phenotype. ERF1 enhances auxin transport by upregulatingPIN1andAUX1, resulting in excessive auxin accumulation in the endodermal, cortical, and epidermal cells surrounding LR primordia. Furthermore, ERF1 repressesARF7transcription, consequently affecting the expression of cell wall remodeling genes that facilitate LR emergence. Together, our study reveals that ERF1 integrates environmental signals to promote local auxin accumulation with altered distribution and repressARF7, consequently inhibiting LR emergence in adaptation to fluctuating environments.HighlightsERF1 functions as a negative regulator of lateral root emergenceERF1 enhances rootward and shootward auxin transport by directly upregulating the expression ofPIN1andAUX1, resulting in high local auxin accumulation and abnormal auxin distribution in the endodermal, cortical, and epidermal cells overlying lateral root primordiaERF1 represses the transcription ofARF7and cell wall remodeling genes in lateral root emergence

show abstract

Section: Introductionmentioning

confidence: 99%

ERF1 inhibits lateral root emergence by promoting local auxin accumulation with altered distribution and repressingARF7expression

Zhao

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…An auxin maximum precedes lateral root formation which depends on auxin transport. Furthermore, the repression of lateral roots in response to loss of contact with water is regulated by movement of ABA from the phloem 87 . Here, we show that genes encoding auxin transporters already show spatially distinct patterns at the time the radicle emerges from the seed coat.…”

Section: Discussionmentioning

confidence: 99%

Spatially Resolved Transcriptomic Analysis of the Germinating Barley Grain

Whelan

Lewsey

Peirats‐Llobet

et al. 2023

Preprint

View full text Add to dashboard Cite

Seeds, which provide a major source of calories for humans, are a unique stage of a flowering plant's lifecycle. During seed germination the embryo reactivates rapidly and goes through major developmental transitions to become a seedling. This requires extensive and complex spatiotemporal coordination of cell and tissue activity. Existing gene expression profiling methods, such as laser capture microdissection followed by RNA-seq and single-cell RNA-seq, suffer from either low throughput or the loss of spatial information about the cells analysed. Spatial transcriptomics methods couple high throughput analysis of gene expression simultaneously with the ability to record the spatial location of each individual region analysed. We developed a spatial transcriptomics workflow for germinating barley grain to better understand the spatiotemporal control of gene expression within individual seed cell types. More than 14,000 genes were differentially regulated across 0, 1, 3, 6 and 24 hours after imbibition. This approach enabled us to observe that many functional categories displayed specific spatial expression patterns that could be resolved at a sub-tissue level. Individual aquaporin gene family members, important for water and ion transport, had specific spatial expression patterns over time, as well as genes related to cell wall modification, membrane transport and transcription factors. Using spatial autocorrelation algorithms, we were able to identify auxin transport genes that had increasingly focused expression within subdomains of the embryo over germination time, suggestive of a role in establishment of the embryo axis. Together, our data provides an unprecedented spatially resolved cellular map for barley grain germination and specific genes to target for functional genomics to define cellular restricted processes in tissues during germination. The data can be viewed at https://spatial.latrobe.edu.au/.

show abstract

“…This auxin gradient is generated by the coordination of PAT and local auxin biosynthesis (Brumos et al., 2018; Petrasek & Friml, 2009; Y. Zhang et al., 2020). The auxin gradient in root tips plays an important role in regulating root growth and development, gravitropic growth, and the response to soil environmental conditions (Giri et al., 2018; Liu et al., 2016; Mehra et al., 2022; Palme et al., 2006; Wang et al., 2019; Y. Zhang et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al, 2020). The auxin gradient in root tips plays an important role in regulating root growth and development, gravitropic growth, and the response to soil environmental conditions (Giri et al, 2018;Liu et al, 2016;Mehra et al, 2022;Palme et al, 2006;Wang et al, 2019;Y. Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis‐ and transport‐mediated dynamic auxin distribution during seed development controls seed size in Arabidopsis

et al. 2023

View full text Add to dashboard Cite

SUMMARY Auxin is indispensable to the fertilization‐induced coordinated development of the embryo, endosperm, and seed coat. However, little attention has been given to the distribution pattern, maintenance mechanism, and function of auxin throughout the process of seed development. In the present study, we found that auxin response signals display a dynamic distribution pattern during Arabidopsis seed development. Shortly after fertilization, strong auxin response signals were observed at the funiculus, chalaza, and micropylar integument where the embryo attaches. Later, additional signals appeared at the middle layer of the inner integument (ii1′) above the chalaza and the whole inner layer of the outer integument (oi1). These signals peaked when the seed was mature, then declined upon desiccation and disappeared in the dried seed. Auxin biosynthesis genes, including ASB1, TAA1, YUC1, YUC4, YUC8, and YUC9, contributed to the accumulation of auxin in the funiculus and seed coat. Auxin efflux carrier PIN3 and influx carrier AUX1 also contributed to the polar auxin distribution in the seed coat. PIN3 was expressed in the ii1 (innermost layer of the inner integument) and oi1 layers of the integument and showed polar localization. AUX1 was expressed in both layers of the outer integument and the endosperm and displayed a uniform localization. Further research demonstrated that the accumulation of auxin in the seed coat regulates seed size. Transgenic plants that specifically express the YUC8 gene in the oi2 or ii1 seed coat produced larger seeds. These results provide useful tools for cultivating high‐yielding crops.

show abstract

Hydraulic flux–responsive hormone redistribution determines root branching

Cited by 46 publications

References 59 publications

ERF1 inhibits lateral root emergence by promoting local auxin accumulation with altered distribution and repressingARF7expression

ERF1 inhibits lateral root emergence by promoting local auxin accumulation with altered distribution and repressingARF7expression

Spatially Resolved Transcriptomic Analysis of the Germinating Barley Grain

Biosynthesis‐ and transport‐mediated dynamic auxin distribution during seed development controls seed size in Arabidopsis

Contact Info

Product

Resources

About