Hydration Study of PEG/Water Mixtures by Quasi Elastic Light Scattering, Acoustic and Rheological Measurements

Branca, C.; Magazù, Salvatore; Maisano, G.; Migliardo, F.; Migliardo, and P.; Romeo, G.

doi:10.1021/jp014345v

Cited by 85 publications

(87 citation statements)

References 21 publications

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“…One can imagine that this relationship covers even a larger range of hydroxyl sites. A similar plot has been reported for PEG/water system where n H evolves "linearly" with the polymerisation degree; however, this study concerned only high-molecular weight polymers [10].…”

Section: Ultrasounds: Determination Of the Hydration Numbersupporting

confidence: 70%

Ultrasonic wave spectroscopy study of sugar oligomers and polysaccharides in aqueous solutions: The hydration length concept

Aeberhardt¹,

Laumer²,

Bouquerand³

et al. 2005

International Journal of Biological Macromolecules

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Section: Ultrasounds: Determination Of the Hydration Numbersupporting

confidence: 70%

Ultrasonic wave spectroscopy study of sugar oligomers and polysaccharides in aqueous solutions: The hydration length concept

Aeberhardt¹,

Laumer²,

Bouquerand³

et al. 2005

International Journal of Biological Macromolecules

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“…Figure 10a shows the variation of the average residence time s Cy3 of the particles in the central stream with f Cy3 for three different PEG loadings (percentage by mass) in the host fluid, e.g., 0, 5, and 10%. The corresponding viscosities of the background liquid, as obtained from the literature (Branca et al 2002), are g = 0.00089, 0.00118, 0.00163 PaÁs, respectively. The vertical error bars in Fig.…”

Section: Binding Ratio In Terms Of Numerically Computed Effective Parmentioning

confidence: 96%

Magnetophoretic mixing for in situ immunochemical binding on magnetic beads in a microfluidic channel

Ganguly

Hahn

Hardt

2009

Microfluid Nanofluid

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Functionalized magnetic beads offer promising solutions to a host of micro-total analysis systems ranging from immunomagnetic biosensors to cell separators. Immunochemical binding of functional biochemical agents or target biomolecules serves as a key step in such applications. Here we show how magnetophoretic motion of magnetic microspheres in a microchannel is harnessed to promote in situ immunochemical binding of short DNA strands (probe oligonucleotide) on the bead surface via streptavidin-biotin bonds. Using a transverse magnetic field gradient, the particles are transported across a co-flowing analyte stream containing biotinylated probe oligonucleotides that are labeled with a Cy3-fluorophore. Quantification of the resulting biotin-streptavidin promoted binding has been achieved through fluorescence imaging of the magnetophoretically separated magnetic particles in a third stream of phosphate buffered saline. Both the experimental and numerical data indicate that for a given flow rate, the analyte binding per bead depends on the flow fraction of the co-flowing analyte stream through the microchannel, but not on the fluid viscosity. Parametric studies of the effects of fluid viscosity, analyte flow fraction, and total flow rate on the extent of binding and the overall analyte separation rate are also conducted numerically to identify favorable operating regimes of a flowthrough immunomagnetic separator for biosensing, cell separation, or high-throughput applications.

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“…We here developed an alternative strategy by substituting the glycans with PEG polymer chains ( Figure 3 A). PEG was chosen for its known biocompatibility and the well-characterized hydration [ 34,35 ] and lubrication [36][37][38][39] capacity. Deglycosylation can decrease the molecular weight of mucins by up to 80%, [ 40,41 ] thus relatively large 40 kDa PEG chains were chosen to compensate for that loss.…”

Section: Peg Can Be Sequestered To Mucins By Conjugation To a Lectinmentioning

confidence: 99%

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

Crouzier

Boettcher

Geonnotti

et al. 2015

Adv Materials Inter

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glycoproteins, a family of high molecular weight and densely glycosylated biopolymers. Those mucins can act as an infection shield by trapping various viruses in the biopolymer matrix [ 1 ] and they reduce bacterial adhesion to surfaces. [ 2,3 ] Mucins also form highly hydrated and lubricative layers that protect the underlying epithelium from dehydration and shear stress that emerge during eye blinking or when swallowing food. Water typically accounts for up to 95% of the total mass in the mucus gel. [ 4,5 ] Shifts in the mucus water content correlate with substantial changes of the mucus barrier function [6][7][8] and result in important pathological disorders such as dry mouth [ 9 ] and dry eye. [ 10 ] Mucin-associated glycans contribute up to 80% of the molecular weight of mucins [ 11 ] and contain highly hydrated hydroxyl groups. Differences in the amount and composition of glycans from mucins extracted from pig stomachs and mucins from bovine submaxillary glands correlate with their capacity to adsorb water. [ 12 ] However, other properties such as protein composition also differ between these two mucin species, precluding the establishment of a defi nitive link between the presence of mucin glycans and hydration.Mucins, and other well-hydrated molecules such as zwitterionic polymers, polysaccharides, and polyelectrolytes are generally good boundary lubricants when adsorbed [ 13,14 ] or assembled in brush structures [15][16][17][18] on surfaces. Hence, mucin-associated glycans could also be essential for the lubricative function of mucus. In a related system, the mucin-like macromolecule lubricin showed a signifi cant decrease in lubricity when its associated glycans were removed. [ 19 ] Moreover, the lubricity of salivary fi lms, which contain mucins, has also been correlated with the total amount of glycosylation present in the fi lm. However, also here, the complexity of saliva composition precluded the identifi cation of the specifi c role of mucin glycosylation. [ 20 ] Hence, there is a need for a more defi ned experimental system to determine if mucin glycosylation is essential to the hydration and lubrication properties of mucins.This study explores the role of glycosylation in mucin hydration and lubricity, using mucin coatings as a simplifi ed model system. Such mucin coatings can reconstitute levels of surface hydration [ 21,22 ] and lubrication [23][24][25] similar to those found in native mucus. Our data show that the removal of mucin-bound glycans results in a signifi cant reduction in hydration and lubricity of the mucin-coated surface. We show that the hydration and lubricity of deglycosylated mucins can be partially A key property of mucin glycoproteins is their exceptional capacity to hydrate and lubricate surfaces. In vivo, mucins assemble into mucus hydrogels that cover the epithelium and protect it from dehydration and shear stress. A better understanding of the origin of these properties could lead to new treatment strategies for patients with poor mucus coverage, defective mucus produ...

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Hydration Study of PEG/Water Mixtures by Quasi Elastic Light Scattering, Acoustic and Rheological Measurements

Cited by 85 publications

References 21 publications

Ultrasonic wave spectroscopy study of sugar oligomers and polysaccharides in aqueous solutions: The hydration length concept

Ultrasonic wave spectroscopy study of sugar oligomers and polysaccharides in aqueous solutions: The hydration length concept

Magnetophoretic mixing for in situ immunochemical binding on magnetic beads in a microfluidic channel

Modulating Mucin Hydration and Lubrication by Deglycosylation and Polyethylene Glycol Binding

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