Hydration of oligosaccharides: Anomalous hydration ability of trehalose

Kawai, Hidetoshi; Sakurai, Minoru; Inoue, Yoshio; Chûjô, Riichirǒ; Kobayashi, Syouichi

doi:10.1016/0011-2240(92)90064-9

Cited by 122 publications

(70 citation statements)

References 27 publications

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“…Although trehalose lacks internal hydrogen bonds, at very high concentrations, the two glucose rings fold over, forming intermolecular hydrogen bonds. 71,72,73 Ultrasonic studies support this hypothesis since the compressibility of trehalose solutions strongly depends on concentration. 72 As water is added, unfolding occurs, making hydrogen bonding sites available for water molecules.…”

Section: Solution Propertiesmentioning

confidence: 86%

“…Indirect evidence for the manipulation of surrounding water layer by trehalose has also come from NMR and ultrasonic studies which reveal that trehalose has a very high affinity for water. 71,72 NMR relaxation time measurements show that trehalose has the highest hydration ability among all the saccharides studied. Although trehalose lacks internal hydrogen bonds, at very high concentrations, the two glucose rings fold over, forming intermolecular hydrogen bonds.…”

Section: Solution Propertiesmentioning

confidence: 95%

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Effect of trehalose on protein structure

Jain¹,

Roy²

2008

Protein Science

709

576

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Trehalose is a ubiquitous molecule that occurs in lower and higher life forms but not in mammals. Till about 40 years ago, trehalose was visualized as a storage molecule, aiding the release of glucose for carrying out cellular functions. This perception has now changed dramatically. The role of trehalose has expanded, and this molecule has now been implicated in a variety of situations. Trehalose is synthesized as a stress-responsive factor when cells are exposed to environmental stresses like heat, cold, oxidation, desiccation, and so forth. When unicellular organisms are exposed to stress, they adapt by synthesizing huge amounts of trehalose, which helps them in retaining cellular integrity. This is thought to occur by prevention of denaturation of proteins by trehalose, which would otherwise degrade under stress. This explanation may be rational, since recently, trehalose has been shown to slow down the rate of polyglutamine-mediated protein aggregation and the resultant pathogenesis by stabilizing an aggregation-prone model protein. In recent years, trehalose has also proved useful in the cryopreservation of sperm and stem cells and in the development of a highly reliable organ preservation solution. This review aims to highlight the changing perception of the role of trehalose over the last 10 years and to propose common mechanisms that may be involved in all the myriad ways in which trehalose stabilizes protein structures. These will take into account the structure of trehalose molecule and its interactions with its environment, and the explanations will focus on the role of trehalose in preventing protein denaturation.

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Section: Solution Propertiesmentioning

confidence: 86%

Section: Solution Propertiesmentioning

confidence: 95%

Effect of trehalose on protein structure

Jain¹,

Roy²

2008

Protein Science

709

576

View full text Add to dashboard Cite

show abstract

“…Among the oligosaccharides, trehalose has the highest ability for hydration, which suggests that trehalose may be stabilizing the bilayer structure of liposomes by ordering the water molecules around the membrane. 252 There are also numerous reports examining the effects of trehalose on the stability of liposomes in solution. [253][254][255] For a more comprehensive description of membrane stabilization, the reader is referred to the work of Crowe and coworkers.…”

Section: Proteins and Enzymesmentioning

confidence: 99%

Trehalose: Current Use and Future Applications

Ohtake

Wang²

2011

Journal of Pharmaceutical Sciences

393

261

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“…It appears that during dehydration or freezing trehalose molecules replace bound water normally associated with biological structures (Donnamaria et al, 1994). Because of its high hydration potential, trehalose may stabilize dry biological membranes and proteins by hydrogen bonding of its hydroxyl groups to the polar groups of proteins and phosphate groups of membranes (Kawai et al, 1992). Another mechanism by which trehalose protects against desiccation stress is vitrification.…”

Section: Desiccation Stressmentioning

confidence: 99%