Hydration Characterization of Two Generations of MTA-Based Root Canal Sealers

Zeid, Sawsan Abu Abu T.; Edrees, Hadeel Y.

doi:10.3390/app12073517

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…Our results for ADSEAL were in agreement with Mostajeran et al (25) , However, they were in contrary with Kim et al, (26) who stated that ADSEAL leads to an increase in cell viability whether it was set or not due to its fast setting time (27) . Previous researches have proved the connection between cytotoxicity and sealer setting time (28)(29)(30) where the unset sealers showed an undefined boundary of decolorized zones and severe cytotoxicity.…”

Section: Discussionsupporting

confidence: 88%

A Laboratory Comparative Study of the Cytotoxic Effect of Bioceramic and Resin Based Root Canal Sealers

Mahfouz,

Abdelbaky,

Omaia

2024

Egyptian Dental Journal

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Objective:The aim of the current study is to evaluate the cytotoxic effect of MTA Bioseal root canal sealer (Itena-clinical, Villepinte, France) with AH + epoxy resin-based sealer (Dentsply Sirona, Germany) and ADSEAL resin sealer (Meta Biomed, Korea). Methods:In sterile test tubes, the tested sealers were consecutively diluted twice. To evaluate the proper concentration where fibroblast cells would survive, extraction media were tempered many times using cell Dulbecco's Modified Eagle's Medium (DMEM) following the ISO standards. The cytotoxic effect of concentrations used in the present study (12.5%, 25%, 50%, and 100%) were evaluated by Methyl Thiazol Tetrazolium (MTT) essay after 24, 72 hours, and one week using a human fibroblast cell line. Statistical analysis was performed by using One Way ANOVA test, followed by Tukey`s Post Hoc test for multiple comparisons.Results: AH Plus and MTA Bioseal showed generally similar behavior. The highest cytotoxicity was recorded for both sealers at conc.100mg/ml in the first 24 hours. Afterwards the mean percentage of cell viability increased progressively with the decrease in concentration. While ADSEAL showed a nearly constant cytotoxicity that is insignificantly affected by the time factor at the four experimental concentrations used in the study. Conclusion:AH Plus had the lowest cytotoxic effect followed by MTA Bioseal. However, both sealers showed parallel behavior of decreased cytotoxicity with increasing test time period. While ADSEAL had the highest cytotoxicity with statistically insignificant effect of time intervals or concentrations.

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Section: Discussionsupporting

confidence: 88%

A Laboratory Comparative Study of the Cytotoxic Effect of Bioceramic and Resin Based Root Canal Sealers

Mahfouz,

Abdelbaky,

Omaia

2024

Egyptian Dental Journal

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“…The in vitro setting times for the sealers varied from 50 min to 8 h, as shown in Table 1. Adseal™ has a short setting time [18]; therefore, it resulted in high cell viability, regardless of whether it was set or unset. This is consistent with the test results of previous studies [19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures

Kim

Hayashi

et al. 2022

Life

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Epoxy resin-based sealers are commonly used for successful endodontic treatment. This study aimed to evaluate the cytotoxicity and genotoxicity of epoxy resin-based sealers under unset and set conditions. Three epoxy resin-based sealers were used: Adseal, AH Plus, and Dia-Proseal. To test cytotoxicity, an agar overlay test and a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay were performed using unset and set sealers on L929 mouse fibroblasts. The genotoxicity test of the comet assay was performed using the same cell line. Extract dilutions in the culture media were used as test materials for the MTT and comet assays. The comet tail produced by the damaged DNA was calculated by image analyses. Statistical analyses were performed using one-way analysis of variance and Tukey’s post hoc test. Unset sealers did not show defined decolorized areas. Hardened specimens of resin-based sealers showed circular discolored zones in the agar overlay test. Dia-Proseal was the least cytotoxic after hardening. These results were confirmed in the MTT assay. Cell viability was significantly higher in cells treated with hardened sealers in both groups than that in cells treated with freshly mixed sealers in the MTT assay. Unset AH Plus® and Dia-Proseal™ significantly increased cell viability with decreasing dilution. Adseal™ was the least cytotoxic. Freshly mixed Adseal™ was more genotoxic when freshly mixed than when set. Unset epoxy resin-based sealers were generally more cytotoxic and genotoxic than set materials. Cytotoxicity does not always match the genotoxicity results; therefore, various test tools are required to test toxicity. It is necessary to properly evaluate the toxic effects to establish a biocompatibility test that mimics clinical conditions.

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“…However, unlike existing MTA, which has a fixed powder-to-liquid ratio, the amount of moisture required for hardening is not separately specified for calcium silicate-based MTA that comes premixed from the manufacturer. Moreover, the manufacturer only indicates that hardening was achieved by the moisture within the root canal [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Comparison of Differences in Setting Time of Premixed Calcium Silicate-Based Mineral Trioxide Aggregate According to Moisture Content of Gypsum

Kim,

Lee,

Gwak

et al. 2023

Materials

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Recently, a paste-type premixed calcium silicate-based mineral trioxide aggregate (MTA) product that quickly solidifies through a pozzolanic reaction was introduced to replace existing MTA, which has the disadvantage of a long setting time. In this study, we evaluated the effect of moisture content in the root canal on the setting time of premixed calcium silicate-based MTA in a simulated root canal environment using Endoseal MTA and Well-Root ST, among commercially available products. The setting time was measured according to ISO 6876/2012. A mold made using grades 2, 3, and 4 dental gypsum according to the classification of ISO 6873/2013 was used to reproduce the difference in moisture environment. Differences in moisture content were measured using micro-computed X-ray tomography (micro-CT). The micro-CT results showed that the moisture content was the highest and lowest in the grade 2 and 4 gypsum molds, respectively. Moreover, the setting time indicated by the manufacturer was the shortest for the grade 2 gypsum mold. Hence, the differences in moisture content significantly affect the setting time of MTA. This result can help set future experimental conditions and develop premixed calcium silicate-based MTA products.

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Hydration Characterization of Two Generations of MTA-Based Root Canal Sealers

Cited by 5 publications

References 45 publications

A Laboratory Comparative Study of the Cytotoxic Effect of Bioceramic and Resin Based Root Canal Sealers

A Laboratory Comparative Study of the Cytotoxic Effect of Bioceramic and Resin Based Root Canal Sealers

Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures

In Vitro Comparison of Differences in Setting Time of Premixed Calcium Silicate-Based Mineral Trioxide Aggregate According to Moisture Content of Gypsum

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