Hydration characteristics of the gum exudate from Acacia senegal

Phillips, Glyn O.; Takigami, Shoji; Takigami, Machiko

doi:10.1016/s0268-005x(96)80048-8

Cited by 40 publications

(18 citation statements)

References 13 publications

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“…We remember that the "hydration number (n h )" refers to water molecules involved in the hydration shell, and then with significant perturbation of its physicochemical properties. These values are in the range of the 0.6-1.2 g H 2 O•g −1 values of interacting "bound" water experimentally measured on Acacia senegal gum by water adsorption experiments or DSC [4,123,126], validating a posteriori the approximations we used for calculations. More specifically, values around 0.6-0.7 g H 2 O•g −1 were found for raw A. senegal gum and values around 0.9-1.2 g H 2 O•g −1 were found for calcium or sodium salts of the gum, showing the role of minerals on molecule hydration.…”

Section: Additional Comments On the Hydration Properties Of Agpssupporting

confidence: 64%

“…Interestingly, like phytoglycogen, hyperbranched AGPs from Acacia gums may adsorb a maximum amount of 3-4 g H 2 O•g −1 AGP, as determined from DSC experiments. This also indicated the formation of a three-dimensional network of AGP and water builds up as a structured entity [123]. We are then faced to an apparent contradiction, since sugars in AGPs displayed the same behavior than single sugars in solution, but a network of interacting water molecules seems to be created.…”

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

“…The question of the hydration of AGPs has not been considered in details in the past, probably because it seemed obvious that Acacia gums are by nature highly hydrophilic [4]. Indeed, the affinity of AGPs for water provides an extremely favorable environment for binding water, which is mainly due to the carbohydrate component of AGPs and in part to their highly branched characteristic [123]. For instance, intramolecular (and intermolecular) voids could be occupied by water in a variety of metastable states, thus preventing the formation of the ideal ice structure [123].…”

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

“…Indeed, the affinity of AGPs for water provides an extremely favorable environment for binding water, which is mainly due to the carbohydrate component of AGPs and in part to their highly branched characteristic [123]. For instance, intramolecular (and intermolecular) voids could be occupied by water in a variety of metastable states, thus preventing the formation of the ideal ice structure [123]. In addition, hyperbranched macromolecules may induce the formation of a well-ordered network of interacting water molecules through the crowded environment between closely packed chains, as shown for phytoglycogen [110,124,125].…”

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

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Flexibility and Hydration of Amphiphilic Hyperbranched Arabinogalactan-Protein from Plant Exudate: A Volumetric Perspective

Tamayo

Nigen

Apolinar-Valiente

et al. 2018

Colloids and Interfaces

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Plant Acacia gum exudates are composed by glycosylated hydroxyproline-rich proteins, which have a high proportion of heavily branched neutral and charged sugars in the polysaccharide moiety. These hyperbranched arabinogalactan-proteins (AGP) display a complexity arising from its composition, architecture, and conformation, but also from its polydispersity and capacity to form supramolecular assemblies. Flexibility and hydration partly determined colloidal and interfacial properties of AGPs. In the present article, these parameters were estimated based on measurements of density and sound velocity and the determination of volumetric parameters, e.g., partial specific volume (v s •) and coefficient of partial specific adiabatic compressibility coefficient (β s •). Measurements were done with Acacia senegal, Acacia seyal, and fractions from the former separated according to their hydrophobicity by Hydrophobic Interaction Chromatography, i.e., HIC-F1, HIC-F2, and HIC-F3. Both gums presented close values of v s • and β s •. However, data on fractions suggested a less hydrated and more flexible structure of HIC-F3, in contrast to a less flexible and more hydrated structure of HIC-F2, and especially HIC-F1. The differences between the macromolecular fractions of A. senegal are significantly related to the fraction composition, protein/polysaccharide ratio, and type of amino acids and sugars, with a polysaccharide moiety mainly contributing to the global hydrophilicity and a protein part mainly contributing to the global hydrophobicity. These properties form the basis of hydration ability and flexibility of hyperbranched AGP from Acacia gums.

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Section: Additional Comments On the Hydration Properties Of Agpssupporting

confidence: 64%

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

Section: Additional Comments On the Hydration Properties Of Agpsmentioning

confidence: 99%

See 2 more Smart Citations

Flexibility and Hydration of Amphiphilic Hyperbranched Arabinogalactan-Protein from Plant Exudate: A Volumetric Perspective

Tamayo

Nigen

Apolinar-Valiente

et al. 2018

Colloids and Interfaces

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“…The model also shows two shallow pockets in AHP-1, the first lined by Gal, GlcUA, and Rha and the second by side chain Gal residue G a and main chain Gal residues G 1-3 . The main chain Gal residues G 0 , G 1 , and G 2 each have a 1,6-linked side chain substituent in close proximity to one another; this results in steric hindrance to their rotation around the 1,6-linkage, further enhanced by the H-bonding, and thus may also stabilize these shallow pockets as putative water-binding sites that may account for the nonfreezing water content identified by differential scanning calorimetry of gum arabic arabinogalactan polysaccharides (43).…”

Section: A Molecular Model Of Ahp-1mentioning

confidence: 99%

Structure of a Hydroxyproline (Hyp)-Arabinogalactan Polysaccharide from Repetitive Ala-Hyp Expressed in Transgenic Nicotiana tabacum

Qiu

Lamport

et al. 2004

Journal of Biological Chemistry

147

149

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A synthetic gene encoding the fusion protein (AlaHyp) 51 -enhanced green fluorescent protein expressed in Nicotiana tabacum cells produced a fusion glycoprotein with all proline residues hydroxylated and substituted with an arabinogalactan polysaccharide. Alkaline hydrolysis of the fusion glycoprotein yielded a population of hydroxyproline (Hyp)-arabinogalactan polysaccharides ranging in size from 13 to 26 saccharide residues/Hyp, with a median size of 15-17 residues. We isolated a 15-residue Hyp-arabinogalactan for structure determination by sugar analyses and one-and two-dimensional nuclear magnetic resonance techniques that provided the assignment of proton and carbon signals of a small polysaccharide O-linked to the hydroxyl group of Hyp. The polysaccharide consisted of a 1,3-linked ␤-D-Galp backbone with a single 1,6-linked ␤-D-Galp ''kink.'' The backbone had two side chains of Galp substituted at position 3 with an arabinose di-or trisaccharide and at position 6 with glucuronic acid or rhamnosyl glucuronic acid. Energy-minimized space-filling molecular models showed hydrogen bonding within polysaccharides attached to repetitive Ala-Hyp and also between polysaccharides and the peptide backbone. Polysaccharides distorted the peptide Ramachandran angles consistent with the circular dichroic spectra of isolated (Ala-Hyp) 51 and its reversion to a polyproline II-like helix after deglycosylation. This first complete structure of a Hyp-arabinogalactan polysaccharide shows that computer-based molecular modeling of Hyp-rich glycoproteins is now feasible and supports the suggestion that small repetitive subunits comprise larger arabinogalactan polysaccharides.

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A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic

et al. 2016

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(2016) A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105 (9). pp. 618-625. ISSN 1097-0282 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/41700/1/Gillisetal%20GA%202016.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk differential press imbalance viscometry) to provide a global analysis of its structure in varying ionic strength conditions. This analysis indicates that gum Arabic may have a compact, elliptical structure in solution, the significance of which for biotechnological use is indicated. This modelling method can be applied to other biopolymers and synthetic polymers.

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Hydration characteristics of the gum exudate from Acacia senegal

Cited by 40 publications

References 13 publications

Flexibility and Hydration of Amphiphilic Hyperbranched Arabinogalactan-Protein from Plant Exudate: A Volumetric Perspective

Flexibility and Hydration of Amphiphilic Hyperbranched Arabinogalactan-Protein from Plant Exudate: A Volumetric Perspective

Structure of a Hydroxyproline (Hyp)-Arabinogalactan Polysaccharide from Repetitive Ala-Hyp Expressed in Transgenic Nicotiana tabacum

A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic

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