Hybridization Study of PNA-DNA in the Solution and Surface-Solution Interface for Biosensor Application

Rastogi, Shiva K.; Østergaard, Michael E.; Cameron, Eric; Finaloski, B; Hrdlicka, Patrick J.; Maki, Wusi C.

doi:10.1080/00032710903137384

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…These results demonstrated that the hybridization between PNA and the DNA was not dependent on FAM functionalization. Several hybridization studies in solution have demonstrated that PNA/complementary ON recognition takes place by Watson–Crick base pairing, that is, A-T and G-C base pairing [ 3 , 46 , 47 , 48 , 49 ]. Therefore, no specific recognition could occur between the synthesized PNA and the non-complementary DNA polyT sequence in solution.…”

Section: Resultsmentioning

confidence: 99%

PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome

et al. 2020

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Peptide nucleic acid (PNA) is a synthetic DNA mimic that outperforms the properties of traditional oligonucleotides (ONs). On account of its outstanding features, such as remarkable binding affinity towards complementary DNA or RNA as well as high thermal and chemical stability, PNA has been proposed as a valuable alternative to the ON probe in gene-sensor design. In this study, a hybrid transducer made-up of graphene oxide (GO) nano-sheets covalently grafted onto a porous silicon (PSi) matrix has been investigated for the early detection of a genetic cardiac disorder, the Brugada syndrome (BS). A functionalization strategy towards the realization of a potential PNA-based device is described. A PNA, able to detect the SCN5A gene associated with the BS, has been properly synthesized and used as a bioprobe for the realization of a proof-of-concept label-free optical PNA-biosensor. PSi reflectance and GO photoluminescence signals were simultaneously exploited for the monitoring of the device functionalization and response.

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Section: Resultsmentioning

confidence: 99%

PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome

et al. 2020

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“…These results demonstrated that the hybridization between PNA and the DNA was not dependent on FAM functionalization. Several hybridization studies in solution have demonstrated that PNA/complementary ON recognition takes place by Watson-Crick base pairing, that is, A-T and G-C base pairing [3,[45][46][47][48]. Therefore, no specific recognition could occur between the synthesized PNA and the non-complementary DNA polyT sequence in solution.…”

Section: Resultsmentioning

confidence: 99%

PNA-based Graphene Oxide/porous Silicon Hybrid Biosensor: Towards a Label-free Optical Assay for Brugada Syndrome

Moretta

Terracciano

Borbone

et al. 2020

Preprint

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Peptide nucleic acid (PNA) is a synthetic DNA mimic that outperforms the properties of traditional oligonucleotides (ONs). On account of its outstanding features, such as remarkable binding affinity towards complementary DNA or RNA as well as high thermal and chemical stability, PNA has been proposed as a valuable alternative to the ON probe in gene-sensor design. In this study, a hybrid transducer made-up of graphene oxide (GO) nano-sheets covalently grafted onto a porous silicon (PSi) matrix has been investigated for the early detection of a genetic cardiac disorder, the Brugada syndrome (BS). A functionalization strategy towards the realization of a potential PNA-based device is described. A peptide nucleic acid (PNA), able to detect the SCN5A associated with the BS has been properly synthesized and used as a bioprobe for the realization of a proof-of-concept label-free optical PNA-biosensor. PSi reflectance and GO photoluminescence (PL) signals were simultaneously exploited for the monitoring of the device functionalization and response.

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“…PNAs have shown to retain their efficient hybridization properties in solution after attachment to the surfaces of substrates. − PNA probes can hybridize with DNA targets in buffers with low ionic strength, which can lower the possibility of forming DNA secondary or tertiary structures. Under physiological conditions, the PNA–DNA duplexes have higher melting temperatures than corresponding DNA–DNA duplexes by approximately 1.5 °C per base pair . In addition, unlike DNA probes that can only detect single-stranded nucleic acid targets, PNA-based probes can bind to double-stranded DNA, − which makes it possible to achieve direct detection of clinical specimens.…”

Section: Introductionmentioning

confidence: 99%

“…Under physiological conditions, the PNA− DNA duplexes have higher melting temperatures than corresponding DNA−DNA duplexes by approximately 1.5 °C per base pair. 17 In addition, unlike DNA probes that can only detect single-stranded nucleic acid targets, PNA-based probes can bind to double-stranded DNA, 18−22 which makes it possible to achieve direct detection of clinical specimens. In fact, analysis of relatively small arrays of PNA oligomers have demonstrate their potential as excellent alternatives to DNA microarrays.…”

Section: ■ Introductionmentioning

confidence: 99%

Light-Directed Synthesis of High-Density Peptide Nucleic Acid Microarrays

et al. 2015

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Peptide nucleic acids (PNAs) are a class of nucleic acid mimics that can bind to the complementary DNA or RNA with high specificity and sensitivity. PNA-based microarrays have distinct characteristics and have improved performance in many aspects compared to DNA microarrays. A new set of PNA monomers has been synthesized and used as the building blocks for the preparation of high density PNA microarrays. These monomers have their backbones protected by the photolabile group 2-(2-nitrophenyl)propyloxy carbonyl (NPPOC), and their exocyclic amino groups protected by amide carbonyl groups. A light-directed synthesis system was designed and applied to the in situ synthesis of a PNA microarray with a density of over 10,000 probes per square centimeter. This PNA microarray was able to detect single and multiple base-mismatches correctly with a high discrimination ratio.

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Hybridization Study of PNA-DNA in the Solution and Surface-Solution Interface for Biosensor Application

Cited by 3 publications

References 15 publications

PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome

PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome

PNA-based Graphene Oxide/porous Silicon Hybrid Biosensor: Towards a Label-free Optical Assay for Brugada Syndrome

Light-Directed Synthesis of High-Density Peptide Nucleic Acid Microarrays

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