Hybridization leads to interspecific gene flow in Sarracenia (Sarraceniaceae)

Furches, M. Steven; Small, Randall L.; Furches, Anna

doi:10.3732/ajb.1300038

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…All recognized species of pitcher plant hybridize with each other, and hybrid pitcher plants can be identified in the field by their characteristics that are intermediate between two non‐hybrid parent species growing sympatrically (Bell, 1952). This long‐practised identification method has more recently been validated by genetic microsatellite analysis (Furches et al ., 2013).…”

Section: Methodsmentioning

confidence: 99%

Sarracenia pitcher plant‐associated microbial communities differ primarily by host species across a longitudinal gradient

Heil

Wolock

Pierce

et al. 2022

Environmental Microbiology

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Plant-associated microbial communities can profoundly affect plant health and success, and research is still uncovering factors driving the assembly of these communities. Here, we examine how geography versus host species affects microbial community structure and differential abundances of individual taxa. We use metabarcoding to characterize the bacteria and eukaryotes associated with five, often cooccurring species of Sarracenia pitcher plants (Sarraceniaceae) and three natural hybrids along the longitudinal gradient of the U.S. Gulf Coast, as well as samples from S. purpurea in Massachusetts. To tease apart the effects of geography versus host species, we focus first on sites with co-occurring species and then on species located across different sites. Our analyses show that bacterial and eukaryotic community structures are clearly and consistently influenced by host species identity, with geographic factors also playing a role. Naturally occurring hybrids appear to also host unique communities, which are in some ways intermediate between their parent species. We see significant effects of geography (site and longitude), but these generally explain less of the variation among pitcher communities. Overall, in Sarracenia pitchers, host plant phenotype significantly affects the pitcher microbiomes and other associated organisms.

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Section: Methodsmentioning

confidence: 99%

Sarracenia pitcher plant‐associated microbial communities differ primarily by host species across a longitudinal gradient

Heil

Wolock

Pierce

et al. 2022

Environmental Microbiology

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“…Most studies on plant RI have focused on only one or a few particular barriers to limit interspecific gene flow, although there are exceptions (e.g., Scopece et al ; Baek et al ; Ma et al ). To determine how species boundaries are maintained between hybridizing species, it is important to understand both the causes and results of hybridization (Furches et al ) and the reproductive barriers that determine the relationship between species boundaries and hybridization of taxa (Widmer et al ; De hert et al ).…”

Section: The Strength Of Each Reproductive Barrier Component and Thementioning

confidence: 99%

Natural hybridization and reproductive isolation between twoPrimulaspecies

Xie

Zhu

et al. 2017

JIPB

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Summary Natural hybridization frequently occurs in plants and can facilitate gene flow between species, possibly resulting in species refusion. However, various reproductive barriers block the formation of hybrids and maintain species integrity. Here, we conducted a field survey to examine natural hybridization and reproductive isolation (RI) between sympatric populations of Primula secundiflora and P. poissonii using ten nuclear simple sequence repeat (SSR) loci. Although introgressive hybridization occurred, species boundaries between P. secundiflora and P. poissonii were maintained through nearly complete reproductive isolation. These interfertile species provide an excellent model for studying the RI mechanisms and evolutionary forces that maintain species boundaries.

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“…These genetically determined characteristics may have allowed pitcher plants to impose control over the microbiome assembly in their pitchers. Thirdly, the various species of Sarracenia can hybridize readily, offering a unique opportunity to incorporate a genetic approach to examine plant genome influences on microbiome assembly (Furches et al 2013). Although pitcher plants have long attracted biologists, we know little about the assembly mechanisms involved in the microbiome assembly and structure in their pitchers (Grothjan and Young 2019).…”

Section: Introductionmentioning

confidence: 99%

The Microbiome and Metatranscriptome of a Panel from theSarraceniaMapping Population Reveal Complex Assembly and Function Involving Host Influence

Cai,

Mohsin,

Rogers

et al. 2024

Preprint

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Sarraceniaprovide an optimal system for deciphering the host-microbiome interactions at various levels. We analyzed the pitcher microbiomes and metatranscriptomes of the parental species, and F1 and F2 generations from the mapping population (Sarracenia purpureaXSarracenia psittacina) utilizing high-throughput sequencing methods. This study aimed to examine the host influences on the microbiome structure and function and to identify the key microbiome traits. Our quality datasets included 8,892,553 full-length bacterial 16s rRNA gene sequences and 65,578 assembled metatranscripts with microbial protein annotations. The correlation network of the bacterial microbiome revealed the presence of 3-7 distinct community clusters, with 8 hub and 19 connector genera. The entire microbiome consisted of viruses, bacterial, archaea, and fungi. The richness and diversity of the microbiome varied among the parental species and offspring genotypes despite being under the same greenhouse environmental conditions. We have discovered certain microbial taxa that are genotype-enriched, including the community hub and connector genera. Nevertheless, there were no significant differences observed in the functional enrichment analysis of the metatranscriptomes across the different genotypes, suggesting a functional convergence of the microbiome. Our results revealed that the pitcher microcosm harbors both rhizosphere and phyllosphere microbiomes within its boundaries, resulting in a structurally diverse and functionally complex microbiome community. A total of 50,424 microbial metatranscripts were linked to plant growth-promoting microbial proteins. We show that this complex pitcher microbiome possesses various functions that contribute to plant growth promotion, such as biofertilization, bioremediation, phytohormone signaling, stress regulation, and immune response stimulation. Additionally, the pitcher microbiome exhibits traits related to microbe-microbe interactions, such as colonization of plant systems, biofilm formation, and microbial competitive exclusion. In summary, the demonstrated taxonomical divergence and functionally convergence of the pitcher microbiome are impacted by the host genetics, making it an excellent system for discovering novel beneficial microbiome traits.

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Hybridization leads to interspecific gene flow in Sarracenia (Sarraceniaceae)

Abstract: Our study provides further evidence that hybridization is contributing to interspecific gene flow in the genus and that all species do not contribute equally to hybridization. Implications for conservation are discussed.

Cited by 9 publications

References 26 publications

Sarracenia pitcher plant‐associated microbial communities differ primarily by host species across a longitudinal gradient

Sarracenia pitcher plant‐associated microbial communities differ primarily by host species across a longitudinal gradient

Natural hybridization and reproductive isolation between twoPrimulaspecies

The Microbiome and Metatranscriptome of a Panel from theSarraceniaMapping Population Reveal Complex Assembly and Function Involving Host Influence

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