Hybridization conditions of oligonucleotide-capped gold nanoparticles for SPR sensing of microRNA

Hong, Long; Lu, Mengdi; Dinel, Marie‐Pier; Blain, Philippe; Peng, Wei; Gu, Hongya; Masson, Jean-François

doi:10.1016/j.bios.2018.03.032

Cited by 47 publications

(26 citation statements)

References 45 publications

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“…Preparation of SPR sensor surfaces can require elaborate procedures (largely depending on the chemistry used for binding the oligo to the gold surface) in order to ensure optimal coverage, probe orientation, and to minimize the surface area of bare-gold sites available for non-specific adsorption [ 30 ]. This is however not always necessary, instead rather simple coating procedures can be sufficient to improve the orientation of the DNA probes and to attenuate non-specific binding, such as a short post-treatment with 6-mercapto-1-hexanol [ 12 ] or using 11-mercaptoundecanoic acid as a linker for immobilizing ssDNA probes [ 31 ]. Surface passivation of thiol-modified morpholino coated sensors can be performed similarly by applying short alkanethiols, but this process requires careful optimization of alkanethiol concentration and incubation time [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…Magnetic beads (usually 1 µm diameter) are routinely used for nucleic acid separation procedures. In SPR systems, usually smaller diameter gold-nanoparticles are used for signal amplification [ 31 , 37 , 38 ]. However, they are more expensive and due to small size are amenable to colloidal aggregation.…”

Section: Resultsmentioning

confidence: 99%

“…Another gold-nanoparticle (20 nm) based signal amplification SPR assay reached 0.01 ng/mL detection limit for 16S rRNA of pathogenic bacteria [ 46 ]. A competition assay, where oligo-conjugated gold nanoparticles competed with the target analyte for the binding sites on the SPR surface reached a similar LOD (0.5 nM) for a 22-nucleotide long target, miRNA-200b as our approach [ 31 ]. The reaction time in their study was 1 h in a stationary mode of a four-channel SPR instrument [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Implementing Morpholino-Based Nucleic Acid Sensing on a Portable Surface Plasmon Resonance Instrument for Future Application in Environmental Monitoring

Bagi

Soelberg

Furlong

et al. 2018

Sensors

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A portable surface plasmon resonance (SPR) instrument was tested for the first time for the detection of oligonucleotide sequences derived from the 16S rRNA gene of Oleispira antarctica RB-8, a bioindicator species of marine oil contamination, using morpholino-functionalized sensor surfaces. We evaluated the stability and specificity of morpholino coated sensor surfaces and tested two signal amplification regimes: (1) sequential injection of sample followed by magnetic bead amplifier and (2) a single injection of magnetic bead captured oligo. We found that the sensor surfaces could be regenerated for at least 85 consecutive sample injections without significant loss of signal intensity. Regarding specificity, the assay clearly differentiated analytes with only one or two mismatches. Signal intensities of mismatch oligos were lower than the exact match target at identical concentrations down to 200 nM, in standard phosphate buffered saline with 0.1 % Tween-20 added. Signal amplification was achieved with both strategies; however, significantly higher response was observed with the sequential approach (up to 16-fold), where first the binding of biotin-probe-labeled target oligo took place on the sensor surface, followed by the binding of the streptavidin magnetic beads onto the immobilized targets. Our experiments so far indicate that a simple coating procedure in combination with a relatively cost-efficient magnetic-bead-based signal amplification will provide robust SPR based nucleic acid sensing down to 0.5 nM of a 45-nucleotide long oligo target (7.2 ng/mL).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Implementing Morpholino-Based Nucleic Acid Sensing on a Portable Surface Plasmon Resonance Instrument for Future Application in Environmental Monitoring

Bagi

Soelberg

Furlong

et al. 2018

Sensors

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“…The hybridization of miRNA target prevented the free oscillation of the DNA probe close to the gold's surface of the sensor causing a change in the signal [56]. SPR techniques were also exploited in a simple combination with AuNPs and hybridization of unamplified miRNA to the surface of the SPR sensor or an optical fiber, offering an LOD of 500 pM and 0.27 pM, respectively [57,58]. An effort was also made by and Zeng et al (2017) to enhance the SPR signal.…”

Section: +mentioning

confidence: 99%

Nanotechnology in emerging liquid biopsy applications

Kalogianni

2021

Nano Convergence

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Liquid biopsy is considered as the most attractive alternative to traditional tissue biopsies. The major advantages of this approach lie in the non-invasive procedure, the rapidness of sample collection and the potential for early cancer diagnosis and real-time monitoring of the disease and the treatment response. Nanotechnology has dynamically emerged in a wide range of applications in the field of liquid biopsy. The benefits of using nanomaterials for biosensing include high sensitivity and detectability, simplicity in many cases, rapid analysis, the low cost of the analysis and the potential for portability and personalized medicine. The present paper reports on the nanomaterial-based methods and biosensors that have been developed for liquid biopsy applications. Most of the nanomaterials used exhibit great analytical performance; moreover, extremely low limits of detection have been achieved for all studied targets. This review will provide scientists with a comprehensive overview of all the nanomaterials and techniques that have been developed for liquid biopsy applications. A comparison of the developed methods in terms of detectability, dynamic range, time-length of the analysis and multiplicity, is also provided.

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“…SPR sensor, which monitors the change in refractive index near the surface during complex formation or dissociation, is one of the most widely used optical sensors [91,113]. Due to its excellent specificity, reproducibility and precision, SPR sensor has been widely applied in miRNA detection [114]. Such method, however, suffers from a major drawback of relative low sensitivity.…”

Section: Signal Transduction and Readout Elementsmentioning

confidence: 99%

Avenues Toward microRNA Detection In Vitro: A Review of Technical Advances and Challenges

Zhu

Tan

et al. 2019

Computational and Structural Biotechnology Journal

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Over the decades, the biological role of microRNAs (miRNAs) in the post-transcriptional regulation of gene expression has been discovered in many cancer types, thus initiating the tremendous expectation of their application as biomarkers in the diagnosis, prognosis, and treatment of cancer. Hence, the development of efficient miRNA detection methods in vitro is in high demand. Extensive efforts have been made based on the intrinsic properties of miRNAs, such as low expression levels, high sequence homology, and short length, to develop novel in vitro miRNA detection methods with high accuracy, low cost, practicality, and multiplexity at point-of-care settings. In this review, we mainly summarized the newly developed in vitro miRNA detection methods classified by three key elements, including biological recognition elements, additional micro-/nano-materials and signal transduction/readout elements, their current challenges and further applications are also discussed.

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Hybridization conditions of oligonucleotide-capped gold nanoparticles for SPR sensing of microRNA

Cited by 47 publications

References 45 publications

Implementing Morpholino-Based Nucleic Acid Sensing on a Portable Surface Plasmon Resonance Instrument for Future Application in Environmental Monitoring

Implementing Morpholino-Based Nucleic Acid Sensing on a Portable Surface Plasmon Resonance Instrument for Future Application in Environmental Monitoring

Nanotechnology in emerging liquid biopsy applications

Avenues Toward microRNA Detection In Vitro: A Review of Technical Advances and Challenges

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